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Protein

Probable rhamnogalacturonase B

Gene

rhgB

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pectinolytic enzymes consist of four classes of enzymes: pectine lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Hydrolyzes alpha-D-galacturonopyranosyl-(1,2)-alpha-L-rhamnopyranosyl linkages in the backbone of the hairy regions of pectins (By similarity).By similarity

Catalytic activityi

Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei219Proton donorBy similarity1
Active sitei294By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable rhamnogalacturonase B (EC:3.2.1.171)
Short name:
RGase B
Short name:
RHG B
Gene namesi
Name:rhgB
ORF Names:AO090010000484
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000039438922 – 532Probable rhamnogalacturonase BAdd BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 68By similarity
Disulfide bondi221 ↔ 238By similarity
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi344 ↔ 350By similarity
Disulfide bondi374 ↔ 383By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ2TWN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217527.
KOiK18580.
OMAiAWALQID.
OrthoDBiEOG092C32CC.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TWN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRINTLSLFS LVSLVPTLAL ASLSGSVGPL TSASTKAATK TCNVLDYGAK
60 70 80 90 100
ADKTTDLGPP LASAFADCKS GGLVYVPSGD YALSTWAKLS GGKAWALQID
110 120 130 140 150
GTIYRTGTDG GNMIFIEHSS DFELFSSTSS GAMQGLGYEY HKDNKWSGPR
160 170 180 190 200
LLRLYDVTDF SVHDFILVDA PAFHFSLDTC TNGEVYNMAI RGGNHGGLDG
210 220 230 240 250
VDVWSTNVWI HDIEVTNKDE CVTVKSPSKN ILVENIYCNW SGGCGMGSLG
260 270 280 290 300
KDTDISDITY RNIYTWNSNN MMFIKSNGGS GSATNLLFEN FIGHGNAYSF
310 320 330 340 350
DIDSYWASMS SQGGNGVELS NITLRNWKGT EENGASRGPI KIVCPDGAPC
360 370 380 390 400
YDITIEDFAM WTEDSSRQRQ WYSCRNAYGT GFCLKSGSNH VAYEATTTTV
410 420 430 440 450
SSAPSGYSAA TMAADLKTDF GITASIPIPT IPTSFYPGAT PYSALMANKG
460 470 480 490 500
STAKVRAVVA PSKPTTVAAA TSTPAEQQTS TSTPAPAVEQ PSQQSPGQSA
510 520 530
GEVGGCPFGN GRSVPTGPPR AGHRHHQRHG HH
Length:532
Mass (Da):56,503
Last modified:January 24, 2006 - v1
Checksum:iC2C4B98299C26FCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007175 Genomic DNA. Translation: BAE66335.1.
RefSeqiXP_001827468.1. XM_001827416.1.

Genome annotation databases

EnsemblFungiiBAE66335; BAE66335; AO090010000484.
GeneIDi5999602.
KEGGiaor:AOR_1_798024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007175 Genomic DNA. Translation: BAE66335.1.
RefSeqiXP_001827468.1. XM_001827416.1.

3D structure databases

ProteinModelPortaliQ2TWN8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAE66335; BAE66335; AO090010000484.
GeneIDi5999602.
KEGGiaor:AOR_1_798024.

Phylogenomic databases

HOGENOMiHOG000217527.
KOiK18580.
OMAiAWALQID.
OrthoDBiEOG092C32CC.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHGB_ASPOR
AccessioniPrimary (citable) accession number: Q2TWN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.