Reviewed, UniProtKB/Swiss-Prot Q2TW34 (KATG_ASPOR)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
ORF Names:	AO090010000722

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	751 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 751	751	Catalase-peroxidase		PRO_0000354102
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	282	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 241		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-267) By similarity
	Cross-link	241 ↔ 267		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TW34-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: FFB1094AA4A555E6	FASTA	751	82,628
	10 20 30 40 50 60 MADKCPFHNQ APKPNVAGSG TQNRDWWPDQ LKLNILRQHT TVSNPLDPDF DYAAAFNSLD 70 80 90 100 110 120 YYALKKDLQD LMTDSQDWWP ADFGHYGGLF IRMAWHSAGT YRTFDGRGGG GQGQQRFAPL 130 140 150 160 170 180 NSWPDNVSLD KARRLLWPIK QKYGNKISWA DLMILTGNVA LESMGFKTFG FAGGRKDTWE 190 200 210 220 230 240 ADESVYWGGE TTWLGNDVRY SHGFAGSSKH GAVIADEASH RDIHSRELEK PLAAAHMGLI 250 260 270 280 290 300 YVNPEGPDGN PDPVAAARDI RTTFARMAMN DEETVALIAG GHTFGKTHGA ASSDHVGSEP 310 320 330 340 350 360 EAAGLEAQGL GWQNSHGSGK GAHTITSGLE VTWTKTPTQW NLNFLEYLFR FEWVLTKSPA 370 380 390 400 410 420 GANQWVAKDA DAFIPDAYDS SKKHRPQMLT TDLSLRFDPA YEKISRRFLE NPDQFAEAFA 430 440 450 460 470 480 RAWFKLTHRD MGPRARYIGP EVPAEELSWQ DPIPAVNHPV ISETDIAALK RDILATGVDP 490 500 510 520 530 540 SKFISTAWAS ASTFRGSDKR GGANGARIRL APQRDWEVNN QPWLAAALKA LEDIQDKFNS 550 560 570 580 590 600 AQNDGKRVSL ADLIVLAGCA AVEKAASDAG HIITVPFTPG RMDASQDQTD VESFNQMEPV 610 620 630 640 650 660 ADGFRNYGTS TARVPAEHYL VDKAQLLTLS APEMTVLVGG LRALNANYDG SAHGVFTTRP 670 680 690 700 710 720 GQLTNDFFVN LLDMNTSWKA SGSGNDIYEG TDRRTGSKKW TATRADLVFG SHAELRAIAE 730 740 750 VYGSSDGKGK FVKDFVAAWA KVMNLDRFDV N

References

[1]

"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP007175 Genomic DNA. Translation: BAE66539.1.
RefSeq	XP_001827672.1.
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	3448. AorCP01.
Genome annotation databases
GeneID	5999806.
GenomeReviews	Gene locus katG in contig AP007175_GR.
KEGG	aor:AO090010000722.
Family and domain databases
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ASPOR

Accession

Primary (citable) accession number: Q2TW34

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 24, 2006
Last modified:	September 22, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)