SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2TSC7

- PCS1_LOTJA

UniProt

Q2TSC7 - PCS1_LOTJA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Glutathione gamma-glutamylcysteinyltransferase 1
Gene
PCS1
Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.

Enzyme regulationi

Requires cadmium for activity. Also activated in vitro by Zn2+, Cu2+, Fe2+ or Fe3+ ions, but not by Co2+ or Ni2+ ions.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561 By similarity
Active sitei162 – 1621 By similarity
Active sitei180 – 1801 By similarity

GO - Molecular functioni

  1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phytochelatin biosynthetic process Source: InterPro
  2. response to metal ion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Cadmium, Copper, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16320.

Protein family/group databases

MEROPSiC83.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)
Alternative name(s):
LjPCS1-8R
Phytochelatin synthase 1
Gene namesi
Name:PCS1
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Glutathione gamma-glutamylcysteinyltransferase 1
PRO_0000287213Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, nodules and leaves.1 Publication

Inductioni

By cadmium.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ2TSC7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 221221Peptidase C83
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2TSC7-1 [UniParc]FASTAAdd to Basket

« Hide

MAMAGLYRRL LPSPPAVDFA SSQGKQLFLE AVQNGTMESF YRLVSYFQTQ    50
SEPAFCGLAS LSMVLNALAI DPGRKWKGPW RWFDESMLDC CEPLDKIKAR 100
GISFGKLVCL AHCAGAKVEA FHASHSSIDH FRKYVMKCST SDDCHVISSY 150
HREALKQTGT GHFSPIGGYH AGKDMALILD VARFKYPPHW IPLTHLWEGM 200
NYVDESTGKT RGFMLISRPH REPGMLYTLS CKHESWNSIA KFLIDDIPFL 250
LTSEDVKDIC KVLSVIVTSL PSNFEEFIKW VAEIRRGEDG SPSLSVEEKA 300
RLSVKEEILK QVQRTGLFKH VASFLSHSCS GHTPTSGDRD TFPVIAASVC 350
CQGAEILGGK ISSSAEYCCR ETCMKCWKAE DDKPIRMVCG TVVNGNTEQG 400
VDVLIPSSCG KLSCTCSSTT KSIRKHPAST DVLTVLLLSL PTSTWAGIAD 450
EKLLSEIHDL VSIENLPALL QEEVLHLRRQ LHILKRCQEG KVDEDLGVPL 500
S 501
Length:501
Mass (Da):55,520
Last modified:January 24, 2006 - v1
Checksum:iEC92820F194A0482
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY633847 mRNA. Translation: AAT80342.1.
AY270030 Genomic DNA. Translation: AAQ01752.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY633847 mRNA. Translation: AAT80342.1 .
AY270030 Genomic DNA. Translation: AAQ01752.1 .

3D structure databases

ProteinModelPortali Q2TSC7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C83.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16320.

Family and domain databases

InterProi IPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view ]
Pfami PF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view ]
PROSITEi PS51443. PCS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A reassessment of substrate specificity and activation of phytochelatin synthases from model plants by physiologically relevant metals."
    Loscos J., Naya L., Ramos J., Clemente M.R., Matamoros M.A., Becana M.
    Plant Physiol. 140:1213-1221(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION.
    Tissue: Root.
  2. "Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
    Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
    Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Miyakojima MG-20.

Entry informationi

Entry nameiPCS1_LOTJA
AccessioniPrimary (citable) accession number: Q2TSC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: January 24, 2006
Last modified: February 19, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In roots, Cd2+ and Zn2+ induce preferentially homophytochelatin (hPC) synthesis whereas Cu2+ has a major effect on phytochelatin (PC) synthesis.
Homoglutathione (hGSH) is a good acceptor, but a poor donor, of gamma-glutamylcysteine units.

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi