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Protein

Glutathione gamma-glutamylcysteinyltransferase 1

Gene

PCS1

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

Miscellaneous

In roots, Cd2+ and Zn2+ induce preferentially homophytochelatin (hPC) synthesis whereas Cu2+ has a major effect on phytochelatin (PC) synthesis.
Homoglutathione (hGSH) is a good acceptor, but a poor donor, of gamma-glutamylcysteine units.

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

Enzyme regulationi

Requires cadmium for activity. Also activated in vitro by Zn2+, Cu2+, Fe2+ or Fe3+ ions, but not by Co2+ or Ni2+ ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei56PROSITE-ProRule annotation1
Active sitei162PROSITE-ProRule annotation1
Active sitei180PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
LigandCadmium, Copper, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16320.
BRENDAi2.3.2.15. 3076.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)
Alternative name(s):
LjPCS1-8R
Phytochelatin synthase 1
Gene namesi
Name:PCS1
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002872131 – 501Glutathione gamma-glutamylcysteinyltransferase 1Add BLAST501

Proteomic databases

PRIDEiQ2TSC7.

Expressioni

Tissue specificityi

Expressed in roots, nodules and leaves.1 Publication

Inductioni

By cadmium.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ2TSC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 221Peptidase C83PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
PfamiView protein in Pfam
PF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
PROSITEiView protein in PROSITE
PS51443. PCS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2TSC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMAGLYRRL LPSPPAVDFA SSQGKQLFLE AVQNGTMESF YRLVSYFQTQ
60 70 80 90 100
SEPAFCGLAS LSMVLNALAI DPGRKWKGPW RWFDESMLDC CEPLDKIKAR
110 120 130 140 150
GISFGKLVCL AHCAGAKVEA FHASHSSIDH FRKYVMKCST SDDCHVISSY
160 170 180 190 200
HREALKQTGT GHFSPIGGYH AGKDMALILD VARFKYPPHW IPLTHLWEGM
210 220 230 240 250
NYVDESTGKT RGFMLISRPH REPGMLYTLS CKHESWNSIA KFLIDDIPFL
260 270 280 290 300
LTSEDVKDIC KVLSVIVTSL PSNFEEFIKW VAEIRRGEDG SPSLSVEEKA
310 320 330 340 350
RLSVKEEILK QVQRTGLFKH VASFLSHSCS GHTPTSGDRD TFPVIAASVC
360 370 380 390 400
CQGAEILGGK ISSSAEYCCR ETCMKCWKAE DDKPIRMVCG TVVNGNTEQG
410 420 430 440 450
VDVLIPSSCG KLSCTCSSTT KSIRKHPAST DVLTVLLLSL PTSTWAGIAD
460 470 480 490 500
EKLLSEIHDL VSIENLPALL QEEVLHLRRQ LHILKRCQEG KVDEDLGVPL

S
Length:501
Mass (Da):55,520
Last modified:January 24, 2006 - v1
Checksum:iEC92820F194A0482
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY633847 mRNA. Translation: AAT80342.1.
AY270030 Genomic DNA. Translation: AAQ01752.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPCS1_LOTJA
AccessioniPrimary (citable) accession number: Q2TSC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: January 24, 2006
Last modified: February 15, 2017
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families