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Q2TSC7

- PCS1_LOTJA

UniProt

Q2TSC7 - PCS1_LOTJA

Protein

Glutathione gamma-glutamylcysteinyltransferase 1

Gene

PCS1

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

    Catalytic activityi

    Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

    Enzyme regulationi

    Requires cadmium for activity. Also activated in vitro by Zn2+, Cu2+, Fe2+ or Fe3+ ions, but not by Co2+ or Ni2+ ions.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei56 – 561PROSITE-ProRule annotation
    Active sitei162 – 1621PROSITE-ProRule annotation
    Active sitei180 – 1801PROSITE-ProRule annotation

    GO - Molecular functioni

    1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. phytochelatin biosynthetic process Source: InterPro
    2. response to metal ion Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Cadmium, Copper, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16320.

    Protein family/group databases

    MEROPSiC83.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)
    Alternative name(s):
    LjPCS1-8R
    Phytochelatin synthase 1
    Gene namesi
    Name:PCS1
    OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
    Taxonomic identifieri34305 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Glutathione gamma-glutamylcysteinyltransferase 1PRO_0000287213Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots, nodules and leaves.1 Publication

    Inductioni

    By cadmium.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ2TSC7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 221221Peptidase C83PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
    Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view]
    PfamiPF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 1 hit.
    [Graphical view]
    PROSITEiPS51443. PCS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2TSC7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMAGLYRRL LPSPPAVDFA SSQGKQLFLE AVQNGTMESF YRLVSYFQTQ    50
    SEPAFCGLAS LSMVLNALAI DPGRKWKGPW RWFDESMLDC CEPLDKIKAR 100
    GISFGKLVCL AHCAGAKVEA FHASHSSIDH FRKYVMKCST SDDCHVISSY 150
    HREALKQTGT GHFSPIGGYH AGKDMALILD VARFKYPPHW IPLTHLWEGM 200
    NYVDESTGKT RGFMLISRPH REPGMLYTLS CKHESWNSIA KFLIDDIPFL 250
    LTSEDVKDIC KVLSVIVTSL PSNFEEFIKW VAEIRRGEDG SPSLSVEEKA 300
    RLSVKEEILK QVQRTGLFKH VASFLSHSCS GHTPTSGDRD TFPVIAASVC 350
    CQGAEILGGK ISSSAEYCCR ETCMKCWKAE DDKPIRMVCG TVVNGNTEQG 400
    VDVLIPSSCG KLSCTCSSTT KSIRKHPAST DVLTVLLLSL PTSTWAGIAD 450
    EKLLSEIHDL VSIENLPALL QEEVLHLRRQ LHILKRCQEG KVDEDLGVPL 500
    S 501
    Length:501
    Mass (Da):55,520
    Last modified:January 24, 2006 - v1
    Checksum:iEC92820F194A0482
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY633847 mRNA. Translation: AAT80342.1.
    AY270030 Genomic DNA. Translation: AAQ01752.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY633847 mRNA. Translation: AAT80342.1 .
    AY270030 Genomic DNA. Translation: AAQ01752.1 .

    3D structure databases

    ProteinModelPortali Q2TSC7.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C83.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16320.

    Family and domain databases

    InterProi IPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view ]
    Pfami PF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 1 hit.
    [Graphical view ]
    PROSITEi PS51443. PCS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A reassessment of substrate specificity and activation of phytochelatin synthases from model plants by physiologically relevant metals."
      Loscos J., Naya L., Ramos J., Clemente M.R., Matamoros M.A., Becana M.
      Plant Physiol. 140:1213-1221(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION.
      Tissue: Root.
    2. "Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
      Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
      Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Miyakojima MG-20.

    Entry informationi

    Entry nameiPCS1_LOTJA
    AccessioniPrimary (citable) accession number: Q2TSC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In roots, Cd2+ and Zn2+ induce preferentially homophytochelatin (hPC) synthesis whereas Cu2+ has a major effect on phytochelatin (PC) synthesis.
    Homoglutathione (hGSH) is a good acceptor, but a poor donor, of gamma-glutamylcysteine units.

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3