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Q2TSC7 (PCS1_LOTJA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione gamma-glutamylcysteinyltransferase 1

EC=2.3.2.15
Alternative name(s):
LjPCS1-8R
Phytochelatin synthase 1
Gene names
Name:PCS1
OrganismLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifier34305 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Ref.2

Catalytic activity

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.

Enzyme regulation

Requires cadmium for activity. Also activated in vitro by Zn2+, Cu2+, Fe2+ or Fe3+ ions, but not by Co2+ or Ni2+ ions. Ref.1

Tissue specificity

Expressed in roots, nodules and leaves. Ref.2

Induction

By cadmium. Ref.1 Ref.2

Miscellaneous

In roots, Cd2+ and Zn2+ induce preferentially homophytochelatin (hPC) synthesis whereas Cu2+ has a major effect on phytochelatin (PC) synthesis.

Homoglutathione (hGSH) is a good acceptor, but a poor donor, of gamma-glutamylcysteine units.

Sequence similarities

Belongs to the phytochelatin synthase family.

Contains 1 peptidase C83 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Glutathione gamma-glutamylcysteinyltransferase 1
PRO_0000287213

Regions

Domain1 – 221221Peptidase C83

Sites

Active site561 By similarity
Active site1621 By similarity
Active site1801 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2TSC7 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: EC92820F194A0482

FASTA50155,520
        10         20         30         40         50         60 
MAMAGLYRRL LPSPPAVDFA SSQGKQLFLE AVQNGTMESF YRLVSYFQTQ SEPAFCGLAS 

        70         80         90        100        110        120 
LSMVLNALAI DPGRKWKGPW RWFDESMLDC CEPLDKIKAR GISFGKLVCL AHCAGAKVEA 

       130        140        150        160        170        180 
FHASHSSIDH FRKYVMKCST SDDCHVISSY HREALKQTGT GHFSPIGGYH AGKDMALILD 

       190        200        210        220        230        240 
VARFKYPPHW IPLTHLWEGM NYVDESTGKT RGFMLISRPH REPGMLYTLS CKHESWNSIA 

       250        260        270        280        290        300 
KFLIDDIPFL LTSEDVKDIC KVLSVIVTSL PSNFEEFIKW VAEIRRGEDG SPSLSVEEKA 

       310        320        330        340        350        360 
RLSVKEEILK QVQRTGLFKH VASFLSHSCS GHTPTSGDRD TFPVIAASVC CQGAEILGGK 

       370        380        390        400        410        420 
ISSSAEYCCR ETCMKCWKAE DDKPIRMVCG TVVNGNTEQG VDVLIPSSCG KLSCTCSSTT 

       430        440        450        460        470        480 
KSIRKHPAST DVLTVLLLSL PTSTWAGIAD EKLLSEIHDL VSIENLPALL QEEVLHLRRQ 

       490        500 
LHILKRCQEG KVDEDLGVPL S 

« Hide

References

[1]"A reassessment of substrate specificity and activation of phytochelatin synthases from model plants by physiologically relevant metals."
Loscos J., Naya L., Ramos J., Clemente M.R., Matamoros M.A., Becana M.
Plant Physiol. 140:1213-1221(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION.
Tissue: Root.
[2]"Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Miyakojima MG-20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY633847 mRNA. Translation: AAT80342.1.
AY270030 Genomic DNA. Translation: AAQ01752.1.

3D structure databases

ProteinModelPortalQ2TSC7.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC83.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16320.

Family and domain databases

InterProIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCS1_LOTJA
AccessionPrimary (citable) accession number: Q2TSC7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: January 24, 2006
Last modified: February 19, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries