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Protein

Hydroxysteroid dehydrogenase-like protein 2

Gene

Hsdl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has apparently no steroid dehydrogenase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421NADPBy similarity
Binding sitei74 – 741NADPBy similarity
Active sitei168 – 1681Proton acceptorSequence analysis
Binding sitei172 – 1721NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 237NADPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxysteroid dehydrogenase-like protein 2 (EC:1.-.-.-)
Gene namesi
Name:Hsdl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1919729. Hsdl2.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: Ensembl
  • mitochondrion Source: MGI
  • peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Hydroxysteroid dehydrogenase-like protein 2PRO_0000319889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161N6-acetyllysineCombined sources
Modified residuei390 – 3901N6-succinyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ2TPA8.
MaxQBiQ2TPA8.
PaxDbiQ2TPA8.
PRIDEiQ2TPA8.

2D gel databases

REPRODUCTION-2DPAGEQ2TPA8.

PTM databases

iPTMnetiQ2TPA8.
PhosphoSiteiQ2TPA8.
SwissPalmiQ2TPA8.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

Up-regulated by cholesterol-rich food.1 Publication

Gene expression databases

BgeeiQ2TPA8.
CleanExiMM_HSDL2.
ExpressionAtlasiQ2TPA8. baseline and differential.
GenevisibleiQ2TPA8. MM.

Interactioni

Protein-protein interaction databases

IntActiQ2TPA8. 2 interactions.
MINTiMINT-1860458.
STRINGi10090.ENSMUSP00000030078.

Structurei

3D structure databases

ProteinModelPortaliQ2TPA8.
SMRiQ2TPA8. Positions 1-276, 383-483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini380 – 487108SCP2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi300 – 37576Gln/Glu/Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 SCP2 domain.Curated

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
KOG4170. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00730000111071.
HOVERGENiHBG107982.
InParanoidiQ2TPA8.
OMAiWFKNHTA.
OrthoDBiEOG77T155.
PhylomeDBiQ2TPA8.
TreeFamiTF101523.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR003033. SCP2_sterol-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF55718. SSF55718. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2TPA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPNTGKLAG CTVFITGASR GIGKAIALKA AKDGANIVIA AKTTQKHPKL
60 70 80 90 100
LGTIYTAAEE IEAAGGTALP CVVDVRDEQQ INSAVEKAVE KFGGIDILVN
110 120 130 140 150
NASAISLTNT LDTPTKRVDL MMNVNTRGTY LTSKACIPFL KKSKVGHILN
160 170 180 190 200
LSPPLNLNPL WFKQHCAYTI AKYGMSMCVL GMAEEFRGEI AVNALWPRTA
210 220 230 240 250
IHTAAMDMLG GSGVENQCRK VDIIADAAYS IFKRPKSFTG NFIIDENILK
260 270 280 290 300
EEGIKNFDVY AIAPGHPLLP DFFLDEHPDA VMEEKESNDS VPEVKEEKLQ
310 320 330 340 350
LQEESQLQKQ PQLQEQPQLQ EKPQLQEKPQ LQEQPQLQEK PQLQEQPQQR
360 370 380 390 400
EQPQLQQQPR PRQQPQPFVQ SMLPQKPHFG AVEETFRIVK DSLSDEVVRA
410 420 430 440 450
TQAVYQFELS GEDGGTWFLD LKSKGGKVGH GEPSDRADVV MSMATDDFVK
460 470 480 490
MFSGKLKPTM AFMSGKLKIK GNIALAIKLE KLMTQMNSRL
Length:490
Mass (Da):54,208
Last modified:January 24, 2006 - v1
Checksum:i700958CDA46905DB
GO

Sequence cautioni

The sequence BAC39563.1 differs from that shown. Reason: Frameshift at position 336. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti307 – 3071L → V in BAE23126 (PubMed:16141072).Curated
Sequence conflicti328 – 3281K → KPQLQEKPQLQEQ in BAE26313 (PubMed:16141072).Curated
Sequence conflicti333 – 3331E → EQPQLQQ in AAH02211 (PubMed:15489334).Curated
Sequence conflicti334 – 3341Q → K in BAE26313 (PubMed:16141072).Curated
Sequence conflicti353 – 3531P → Q in BAE26313 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY725196 mRNA. Translation: AAU11505.1.
AK085899 mRNA. Translation: BAC39563.1. Frameshift.
AK136773 mRNA. Translation: BAE23126.1.
AK145230 mRNA. Translation: BAE26313.1.
AL806512, BX005031 Genomic DNA. Translation: CAM21909.1.
BX005031, AL806512 Genomic DNA. Translation: CAM26657.1.
BC002211 mRNA. Translation: AAH02211.1.
CCDSiCCDS38772.1.
RefSeqiNP_077217.2. NM_024255.3.
UniGeneiMm.272905.

Genome annotation databases

EnsembliENSMUST00000030078; ENSMUSP00000030078; ENSMUSG00000028383.
GeneIDi72479.
KEGGimmu:72479.
UCSCiuc008szy.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY725196 mRNA. Translation: AAU11505.1.
AK085899 mRNA. Translation: BAC39563.1. Frameshift.
AK136773 mRNA. Translation: BAE23126.1.
AK145230 mRNA. Translation: BAE26313.1.
AL806512, BX005031 Genomic DNA. Translation: CAM21909.1.
BX005031, AL806512 Genomic DNA. Translation: CAM26657.1.
BC002211 mRNA. Translation: AAH02211.1.
CCDSiCCDS38772.1.
RefSeqiNP_077217.2. NM_024255.3.
UniGeneiMm.272905.

3D structure databases

ProteinModelPortaliQ2TPA8.
SMRiQ2TPA8. Positions 1-276, 383-483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ2TPA8. 2 interactions.
MINTiMINT-1860458.
STRINGi10090.ENSMUSP00000030078.

PTM databases

iPTMnetiQ2TPA8.
PhosphoSiteiQ2TPA8.
SwissPalmiQ2TPA8.

2D gel databases

REPRODUCTION-2DPAGEQ2TPA8.

Proteomic databases

EPDiQ2TPA8.
MaxQBiQ2TPA8.
PaxDbiQ2TPA8.
PRIDEiQ2TPA8.

Protocols and materials databases

DNASUi72479.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030078; ENSMUSP00000030078; ENSMUSG00000028383.
GeneIDi72479.
KEGGimmu:72479.
UCSCiuc008szy.3. mouse.

Organism-specific databases

CTDi84263.
MGIiMGI:1919729. Hsdl2.

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
KOG4170. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00730000111071.
HOVERGENiHBG107982.
InParanoidiQ2TPA8.
OMAiWFKNHTA.
OrthoDBiEOG77T155.
PhylomeDBiQ2TPA8.
TreeFamiTF101523.

Miscellaneous databases

PROiQ2TPA8.
SOURCEiSearch...

Gene expression databases

BgeeiQ2TPA8.
CleanExiMM_HSDL2.
ExpressionAtlasiQ2TPA8. baseline and differential.
GenevisibleiQ2TPA8. MM.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR003033. SCP2_sterol-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
PF02036. SCP2. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF55718. SSF55718. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel mouse short-chain dehydrogenase/reductases cDNA mHsdl2, encoding a protein with a SDR domain and a SCP2 domain."
    Dai J., Li P., Ji C., Feng C., Gui M., Sun Y., Zhang J., Zhu J., Dou C., Gu S.
    Mol. Biol. (Mosk.) 39:799-805(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Diencephalon, Heart and Mammary gland.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-390, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-116, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiHSDL2_MOUSE
AccessioniPrimary (citable) accession number: Q2TPA8
Secondary accession number(s): Q3ULY5
, Q3UVZ3, Q8C3H3, Q99LV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 24, 2006
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.