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Q2TE74

- PCS2_LOTJA

UniProt

Q2TE74 - PCS2_LOTJA

Protein

Glutathione gamma-glutamylcysteinyltransferase 2

Gene

PCS2

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 2 (15 May 2007)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

    Catalytic activityi

    Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

    Enzyme regulationi

    Requires cadmium for activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei56 – 561PROSITE-ProRule annotation
    Active sitei162 – 1621PROSITE-ProRule annotation
    Active sitei180 – 1801PROSITE-ProRule annotation

    GO - Molecular functioni

    1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. phytochelatin biosynthetic process Source: InterPro
    2. response to metal ion Source: InterPro

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Cadmium, Metal-binding

    Protein family/group databases

    MEROPSiC83.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione gamma-glutamylcysteinyltransferase 2 (EC:2.3.2.15)
    Alternative name(s):
    LjPCS2-7R
    Phytochelatin synthase 2
    Gene namesi
    Name:PCS2
    OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
    Taxonomic identifieri34305 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482Glutathione gamma-glutamylcysteinyltransferase 2PRO_0000287214Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots, nodules and leaves.1 Publication

    Inductioni

    By cadmium.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ2TE74.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 221221Peptidase C83PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi352 – 37120Cys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
    Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view]
    PfamiPF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 1 hit.
    [Graphical view]
    PROSITEiPS51443. PCS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Ljap2N (identifier: Q2TE74-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASVSLYRRV LPSPPAIDFA SQEGKKIFVE ALGQGTMEGF FKLISFYQTQ    50
    SEPAYCGLAT LSVVLNALAI DPGRKWKGPW RWFDDSMLDC CEPLEKVKVQ 100
    GITFGKVACL ARCNGAKVEA FRSNESTVSD FRNRVISCCS SEDRHAIVSY 150
    HRSGLKQTGE GHFSPLGGYH AERDMVLILD VTRYKYPPHW VPVTLLWNAM 200
    NTIDRATGLQ RGYMIISKLN RAPSILYTLS CRHEGWSSVA KFLTENVPLL 250
    LKSEDLKDIQ EVLSVVFKSP PSELREFITW IAEVRRQEDG NLTLSEEEKG 300
    RLVIKAEILE KIRTAGLFKH VTSWLDSQRS RCSTIANLQD KDMLPELAAR 350
    VCCQGACLLT GCCLSGGKCC CQIDVKHLNV DSKNLATTFV SGTFTTGSSS 400
    EQGVDVLVPL CQRGPEGHYI AMHPSTADVL TVLLLALPLH TWSGIKEEKL 450
    RAEVTSLVTT EDLPSLLQEE VILYMEPVLQ FF 482

    Note: Found in nodules.

    Length:482
    Mass (Da):53,692
    Last modified:May 15, 2007 - v2
    Checksum:iBE6368B067F9DAE0
    GO
    Isoform Ljap2R (identifier: Q2TE74-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         73-77: Missing.

    Note: Found in roots. Not functional in yeast heterologous system.

    Show »
    Length:477
    Mass (Da):53,036
    Checksum:iDF7D691AF7F8B802
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei73 – 775Missing in isoform Ljap2R. 1 PublicationVSP_025376

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY633846 mRNA. Translation: AAT80341.1.
    AY928091 Genomic DNA. Translation: AAX99139.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY633846 mRNA. Translation: AAT80341.1 .
    AY928091 Genomic DNA. Translation: AAX99139.1 .

    3D structure databases

    ProteinModelPortali Q2TE74.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C83.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007719. Phytochelatin_synthase.
    IPR015407. Phytochelatin_synthase_C.
    [Graphical view ]
    Pfami PF05023. Phytochelatin. 1 hit.
    PF09328. Phytochelatin_C. 1 hit.
    [Graphical view ]
    PROSITEi PS51443. PCS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Lotus japonicus phytochelatin synthase mRNA (pcs2)."
      Naya L., Ramos J., Becana M.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LJAP2R).
      Tissue: Root.
    2. "Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
      Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
      Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Miyakojima MG-20.

    Entry informationi

    Entry nameiPCS2_LOTJA
    AccessioniPrimary (citable) accession number: Q2TE74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 32 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3