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Q2TE74 (PCS2_LOTJA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione gamma-glutamylcysteinyltransferase 2

EC=2.3.2.15
Alternative name(s):
LjPCS2-7R
Phytochelatin synthase 2
Gene names
Name:PCS2
OrganismLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifier34305 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Ref.2

Catalytic activity

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.

Enzyme regulation

Requires cadmium for activity By similarity.

Tissue specificity

Expressed in roots, nodules and leaves. Ref.2

Induction

By cadmium. Ref.2

Sequence similarities

Belongs to the phytochelatin synthase family.

Contains 1 peptidase C83 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Ljap2N (identifier: Q2TE74-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Found in nodules.
Isoform Ljap2R (identifier: Q2TE74-2)

The sequence of this isoform differs from the canonical sequence as follows:
     73-77: Missing.
Note: Found in roots. Not functional in yeast heterologous system.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482Glutathione gamma-glutamylcysteinyltransferase 2
PRO_0000287214

Regions

Domain1 – 221221Peptidase C83
Compositional bias352 – 37120Cys-rich

Sites

Active site561 By similarity
Active site1621 By similarity
Active site1801 By similarity

Natural variations

Alternative sequence73 – 775Missing in isoform Ljap2R.
VSP_025376

Sequences

Sequence LengthMass (Da)Tools
Isoform Ljap2N [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: BE6368B067F9DAE0

FASTA48253,692
        10         20         30         40         50         60 
MASVSLYRRV LPSPPAIDFA SQEGKKIFVE ALGQGTMEGF FKLISFYQTQ SEPAYCGLAT 

        70         80         90        100        110        120 
LSVVLNALAI DPGRKWKGPW RWFDDSMLDC CEPLEKVKVQ GITFGKVACL ARCNGAKVEA 

       130        140        150        160        170        180 
FRSNESTVSD FRNRVISCCS SEDRHAIVSY HRSGLKQTGE GHFSPLGGYH AERDMVLILD 

       190        200        210        220        230        240 
VTRYKYPPHW VPVTLLWNAM NTIDRATGLQ RGYMIISKLN RAPSILYTLS CRHEGWSSVA 

       250        260        270        280        290        300 
KFLTENVPLL LKSEDLKDIQ EVLSVVFKSP PSELREFITW IAEVRRQEDG NLTLSEEEKG 

       310        320        330        340        350        360 
RLVIKAEILE KIRTAGLFKH VTSWLDSQRS RCSTIANLQD KDMLPELAAR VCCQGACLLT 

       370        380        390        400        410        420 
GCCLSGGKCC CQIDVKHLNV DSKNLATTFV SGTFTTGSSS EQGVDVLVPL CQRGPEGHYI 

       430        440        450        460        470        480 
AMHPSTADVL TVLLLALPLH TWSGIKEEKL RAEVTSLVTT EDLPSLLQEE VILYMEPVLQ 


FF 

« Hide

Isoform Ljap2R [UniParc].

Checksum: DF7D691AF7F8B802
Show »

FASTA47753,036

References

[1]"Lotus japonicus phytochelatin synthase mRNA (pcs2)."
Naya L., Ramos J., Becana M.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LJAP2R).
Tissue: Root.
[2]"Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Miyakojima MG-20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY633846 mRNA. Translation: AAT80341.1.
AY928091 Genomic DNA. Translation: AAX99139.1.

3D structure databases

ProteinModelPortalQ2TE74.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC83.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCS2_LOTJA
AccessionPrimary (citable) accession number: Q2TE74
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: February 19, 2014
This is version 31 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries