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Q2TE74

- PCS2_LOTJA

UniProt

Q2TE74 - PCS2_LOTJA

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Protein

Glutathione gamma-glutamylcysteinyltransferase 2

Gene

PCS2

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

Enzyme regulationi

Requires cadmium for activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561PROSITE-ProRule annotation
Active sitei162 – 1621PROSITE-ProRule annotation
Active sitei180 – 1801PROSITE-ProRule annotation

GO - Molecular functioni

  1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phytochelatin biosynthetic process Source: InterPro
  2. response to metal ion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Cadmium, Metal-binding

Protein family/group databases

MEROPSiC83.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 2 (EC:2.3.2.15)
Alternative name(s):
LjPCS2-7R
Phytochelatin synthase 2
Gene namesi
Name:PCS2
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Glutathione gamma-glutamylcysteinyltransferase 2PRO_0000287214Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, nodules and leaves.1 Publication

Inductioni

By cadmium.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ2TE74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 221221Peptidase C83PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi352 – 37120Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Ljap2N (identifier: Q2TE74-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVSLYRRV LPSPPAIDFA SQEGKKIFVE ALGQGTMEGF FKLISFYQTQ
60 70 80 90 100
SEPAYCGLAT LSVVLNALAI DPGRKWKGPW RWFDDSMLDC CEPLEKVKVQ
110 120 130 140 150
GITFGKVACL ARCNGAKVEA FRSNESTVSD FRNRVISCCS SEDRHAIVSY
160 170 180 190 200
HRSGLKQTGE GHFSPLGGYH AERDMVLILD VTRYKYPPHW VPVTLLWNAM
210 220 230 240 250
NTIDRATGLQ RGYMIISKLN RAPSILYTLS CRHEGWSSVA KFLTENVPLL
260 270 280 290 300
LKSEDLKDIQ EVLSVVFKSP PSELREFITW IAEVRRQEDG NLTLSEEEKG
310 320 330 340 350
RLVIKAEILE KIRTAGLFKH VTSWLDSQRS RCSTIANLQD KDMLPELAAR
360 370 380 390 400
VCCQGACLLT GCCLSGGKCC CQIDVKHLNV DSKNLATTFV SGTFTTGSSS
410 420 430 440 450
EQGVDVLVPL CQRGPEGHYI AMHPSTADVL TVLLLALPLH TWSGIKEEKL
460 470 480
RAEVTSLVTT EDLPSLLQEE VILYMEPVLQ FF

Note: Found in nodules.

Length:482
Mass (Da):53,692
Last modified:May 15, 2007 - v2
Checksum:iBE6368B067F9DAE0
GO
Isoform Ljap2R (identifier: Q2TE74-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     73-77: Missing.

Note: Found in roots. Not functional in yeast heterologous system.

Show »
Length:477
Mass (Da):53,036
Checksum:iDF7D691AF7F8B802
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei73 – 775Missing in isoform Ljap2R. 1 PublicationVSP_025376

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY633846 mRNA. Translation: AAT80341.1.
AY928091 Genomic DNA. Translation: AAX99139.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY633846 mRNA. Translation: AAT80341.1 .
AY928091 Genomic DNA. Translation: AAX99139.1 .

3D structure databases

ProteinModelPortali Q2TE74.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C83.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view ]
Pfami PF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view ]
PROSITEi PS51443. PCS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Lotus japonicus phytochelatin synthase mRNA (pcs2)."
    Naya L., Ramos J., Becana M.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LJAP2R).
    Tissue: Root.
  2. "Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
    Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
    Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Miyakojima MG-20.

Entry informationi

Entry nameiPCS2_LOTJA
AccessioniPrimary (citable) accession number: Q2TE74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: October 1, 2014
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3