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Protein

Sorting nexin-2

Gene

SNX2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Required for retrograde endosome-to-TGN transport of TGN38. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei183Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei185Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei211Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei235Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-2
Gene namesi
Name:SNX2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Early endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity
  • Cell projectionlamellipodium

  • Note: Colocalized with SORT1 to tubular endosomal membrane structures called endosome-to-TGN transport carriers (ETCs) which are budding from early endosome vacuoles just before maturing into late endosome vacuoles. Colocalized with F-actin at the leading edge of lamellipodia in cells in a KALRN-dependent manner (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002454881 – 519Sorting nexin-2Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97PhosphoserineBy similarity1
Modified residuei101PhosphothreonineBy similarity1
Modified residuei104PhosphothreonineBy similarity1
Modified residuei117PhosphoserineBy similarity1
Modified residuei119PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei277PhosphoserineBy similarity1
Modified residuei469N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2TBW7.
PeptideAtlasiQ2TBW7.
PRIDEiQ2TBW7.

Interactioni

Subunit structurei

Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32; can self-associate to form homodimers. The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also decribed as vacuolar protein sorting subcomplex (VPS) and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, FNBP1, KALRN, RHOG (GDP-bound form) (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035453.

Structurei

3D structure databases

ProteinModelPortaliQ2TBW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 269PXPROSITE-ProRule annotationAdd BLAST130
Domaini299 – 519BARBy similarityAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 519Interaction with RhoGBy similarityAdd BLAST260
Regioni278 – 295Membrane-binding amphipathic helixBy similarityAdd BLAST18

Domaini

The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of a N-terminal amphipatric helix (AH) in the membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 BAR domain.By similarity
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2273. Eukaryota.
COG5391. LUCA.
HOGENOMiHOG000293327.
HOVERGENiHBG000618.
InParanoidiQ2TBW7.
KOiK17917.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR028653. SNX2.
IPR005329. Sorting_nexin_N.
IPR015404. Vps5_C.
[Graphical view]
PANTHERiPTHR10555:SF31. PTHR10555:SF31. 1 hit.
PfamiPF00787. PX. 1 hit.
PF03700. Sorting_nexin. 1 hit.
PF09325. Vps5. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2TBW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEREPPPL GDGKPTDFEE LEDGEDLFTS TVSTLESSPS SPDPASFLAE
60 70 80 90 100
DISTNSNGPK PAEVALDDDR EDLFAEATEE VSLDSPEREP ILSSETSPAV
110 120 130 140 150
TPVTPTTLIA PRIESKSMSA PVIFDRSRDE IEEEANGDVF DIEIGVSDPE
160 170 180 190 200
KVGDGMNAYM AYRVTTKTSL SMFSKSEFSV KRRFSDFLGL HSKLASKYLH
210 220 230 240 250
VGYIVPPAPE KSIVGMTKVK VGKEDSSSTE FVEKRRAALE RYLQRTVKHP
260 270 280 290 300
TLLQDPDLRQ FLESSELPRA VNTQALSGAG ILRMVNKAAD AVNKMTIKMN
310 320 330 340 350
ESDAWFEEKQ QQFENQDQQL RKLHASVEAL VCHRKELSAN TAAFAKSAAM
360 370 380 390 400
LGNSEDHTAL SRALSQLAEV EEKIDQLHQE QAFADFYMFS ELLSDYIRLI
410 420 430 440 450
AAVKGVFDHR VKCWQKWEDA QITLLKKRET EAKMMVANKP DKIQQAKNEI
460 470 480 490 500
REWEAKVQQG ERDFEQISKT IRKEVGRFEK ERVKDFKTVI IKYLESLVQT
510
QQQLIKYWEA FLPEAKAIA
Length:519
Mass (Da):58,454
Last modified:January 24, 2006 - v1
Checksum:i05A9E48C455167D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109562 mRNA. Translation: AAI09563.1.
RefSeqiNP_001033608.1. NM_001038519.1.
UniGeneiBt.26946.

Genome annotation databases

GeneIDi509769.
KEGGibta:509769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109562 mRNA. Translation: AAI09563.1.
RefSeqiNP_001033608.1. NM_001038519.1.
UniGeneiBt.26946.

3D structure databases

ProteinModelPortaliQ2TBW7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035453.

Proteomic databases

PaxDbiQ2TBW7.
PeptideAtlasiQ2TBW7.
PRIDEiQ2TBW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi509769.
KEGGibta:509769.

Organism-specific databases

CTDi6643.

Phylogenomic databases

eggNOGiKOG2273. Eukaryota.
COG5391. LUCA.
HOGENOMiHOG000293327.
HOVERGENiHBG000618.
InParanoidiQ2TBW7.
KOiK17917.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR028653. SNX2.
IPR005329. Sorting_nexin_N.
IPR015404. Vps5_C.
[Graphical view]
PANTHERiPTHR10555:SF31. PTHR10555:SF31. 1 hit.
PfamiPF00787. PX. 1 hit.
PF03700. Sorting_nexin. 1 hit.
PF09325. Vps5. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNX2_BOVIN
AccessioniPrimary (citable) accession number: Q2TBW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 24, 2006
Last modified: October 5, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.