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Protein

ADP-ribosylation factor-like protein 3

Gene

ARL3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31MagnesiumBy similarity1
Metal bindingi48MagnesiumBy similarity1
Binding sitei48GTPBy similarity1
Binding sitei70GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTPBy similarity8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4
Nucleotide bindingi159 – 161GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Protein transport, Transport
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 3
Gene namesi
Name:ARL3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002453542 – 182ADP-ribosylation factor-like protein 3Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei5PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ2TBW6.
PRIDEiQ2TBW6.

Interactioni

Subunit structurei

Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. Interacts with ARL2BP; the GTP-bound form interacts with ARL2BP. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC. Interacts with RP2 and PDE6D. Interacts with PDE6D; the interaction occurs specifically with the GTP-bound form of ARL3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005655.

Structurei

3D structure databases

ProteinModelPortaliQ2TBW6.
SMRiQ2TBW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
HOVERGENiHBG002073.
InParanoidiQ2TBW6.
KOiK07944.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
PfamiView protein in Pfam
PF00025. Arf. 1 hit.
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51417. ARF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TBW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG
60 70 80 90 100
FNIKSVQSQG FKLNVWDIGG QRKIRPYWRN YFENTDILIY VIDSADRKRF
110 120 130 140 150
EETGQELAEL LEEEKLSCVP VLIFANKQDL LTAAPASEIA EGLNLHTIRD
160 170 180
RFWQIQSCSA LTGEGVQDGM NWVCKNVSAK KK
Length:182
Mass (Da):20,505
Last modified:January 24, 2006 - v1
Checksum:i8F326391E9F4A920
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109565 mRNA. Translation: AAI09566.1.
RefSeqiNP_001033656.1. NM_001038567.2.
UniGeneiBt.3641.

Genome annotation databases

GeneIDi540040.
KEGGibta:540040.

Similar proteinsi

Entry informationi

Entry nameiARL3_BOVIN
AccessioniPrimary (citable) accession number: Q2TBW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 24, 2006
Last modified: August 30, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families