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Protein

Ribonuclease H2 subunit A

Gene

RNASEH2A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Divalent metal cationBy similarity1
Metal bindingi35Divalent metal cationBy similarity1
Metal bindingi142Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit A (EC:3.1.26.4)
Short name:
RNase H2 subunit A
Alternative name(s):
Ribonuclease HI large subunit
Short name:
RNase HI large subunit
Ribonuclease HI subunit A
Gene namesi
Name:RNASEH2A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002473211 – 299Ribonuclease H2 subunit AAdd BLAST299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei205PhosphothreonineBy similarity1
Modified residuei217PhosphothreonineBy similarity1
Modified residuei258PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2TBT5.
PRIDEiQ2TBT5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000009661.

Interactioni

Subunit structurei

The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012731.

Structurei

3D structure databases

ProteinModelPortaliQ2TBT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. Eukaryotic subfamily.Curated

Phylogenomic databases

eggNOGiKOG2299. Eukaryota.
COG0164. LUCA.
GeneTreeiENSGT00390000010768.
HOGENOMiHOG000100290.
HOVERGENiHBG023585.
InParanoidiQ2TBT5.
KOiK10743.
OMAiREECRFF.
OrthoDBiEOG091G0E06.
TreeFamiTF314302.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2TBT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLSELERDN TGRCRLSSPV PPVCLKEPCV LGVDEAGRGP VLGPMVYAIC
60 70 80 90 100
YCPLSRLEDL EALKVADSKT LSESERDRLF AKMEEDGDFV GWALDVLSPN
110 120 130 140 150
LISTSMLGRV KYNLNALSHD TATGLVQFAL DQGVNVAQVF VDTVGLPETY
160 170 180 190 200
QERLQQRFPG IEVTVKAKAD ALYPVVSAAS ICAKVARDQA VKNWKFVEKL
210 220 230 240 250
QDLDTDYGSG YPNDPKTKAW LRKHVDPVFG FPQFVRFSWR TAQSILESEA
260 270 280 290
EDVKWEDSET GDPKGPGKIK SYFSESPQTC LRLPHRYFQE RGLESATVL
Length:299
Mass (Da):33,300
Last modified:January 24, 2006 - v1
Checksum:i902422B95D793608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109682 mRNA. Translation: AAI09683.1.
RefSeqiNP_001033623.1. NM_001038534.2.
UniGeneiBt.44367.

Genome annotation databases

EnsembliENSBTAT00000012731; ENSBTAP00000012731; ENSBTAG00000009661.
GeneIDi512830.
KEGGibta:512830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109682 mRNA. Translation: AAI09683.1.
RefSeqiNP_001033623.1. NM_001038534.2.
UniGeneiBt.44367.

3D structure databases

ProteinModelPortaliQ2TBT5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012731.

Proteomic databases

PaxDbiQ2TBT5.
PRIDEiQ2TBT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012731; ENSBTAP00000012731; ENSBTAG00000009661.
GeneIDi512830.
KEGGibta:512830.

Organism-specific databases

CTDi10535.

Phylogenomic databases

eggNOGiKOG2299. Eukaryota.
COG0164. LUCA.
GeneTreeiENSGT00390000010768.
HOGENOMiHOG000100290.
HOVERGENiHBG023585.
InParanoidiQ2TBT5.
KOiK10743.
OMAiREECRFF.
OrthoDBiEOG091G0E06.
TreeFamiTF314302.

Gene expression databases

BgeeiENSBTAG00000009661.

Family and domain databases

Gene3Di1.10.10.460. 1 hit.
3.30.420.10. 1 hit.
InterProiIPR004649. RNase_H2_suA.
IPR001352. RNase_HII/HIII.
IPR024567. RNase_HII/HIII_dom.
IPR023160. RNase_HII_hlx-loop-hlx_cap_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10954. PTHR10954. 1 hit.
PfamiPF01351. RNase_HII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00729. TIGR00729. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNH2A_BOVIN
AccessioniPrimary (citable) accession number: Q2TBT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 24, 2006
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.