Q2TBR8 (CALR3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calreticulin-3

Alternative name(s):
Calsperin

Gene names

Name:

CALR3

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. CALR3 capacity for calcium-binding may be absent or much lower than that of CALR. Required for sperm fertility By similarity.
Subunit structure	Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: InterPro spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 384	365	Calreticulin-3		PRO_0000282867
Regions
	Repeat	191 – 202	12	1-1
	Repeat	209 – 220	12	1-2
	Repeat	222 – 231	10	1-3
	Repeat	235 – 246	12	1-4
	Repeat	250 – 260	11	2-1
	Repeat	264 – 272	9	2-2
	Repeat	274 – 284	11	2-3
	Region	20 – 197	178	N-domain
	Region	191 – 246	56	4 X approximate repeats
	Region	198 – 294	97	P-domain
	Region	250 – 284	35	3 X approximate repeats
	Region	295 – 384	90	C-domain
	Motif	381 – 384	4	Prevents secretion from ER Potential
	Compositional bias	337 – 362	26	Glu/Lys-rich
Sites
	Binding site	109	1	Carbohydrate By similarity
	Binding site	111	1	Carbohydrate By similarity
	Binding site	128	1	Carbohydrate By similarity
	Binding site	135	1	Carbohydrate By similarity
	Binding site	303	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Disulfide bond	105 ↔ 137		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TBR8 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: BAE01C8F353718C2	FASTA	384	44,512
	10 20 30 40 50 60 MAAARVPLWA ICVRRVALAT VYFQEEFLDG ERWRNRWVHS TNDSQFGHFR LSSGNFYGHK 70 80 90 100 110 120 EKDKGLQTTQ NSRFYAISAR FKPFSNKGKT LIIQYTVKHE QKMDCGGGYI KLFPADVDQK 130 140 150 160 170 180 NLNGKSQYYI MFGPDICGFD IKTVHVILHF KNQYHANKKS IRCKVDSFTH LYTLVLRPDL 190 200 210 220 230 240 TYEVKIDGQS IESGSIEYDW QLTSLKKMEK ASAEAEGWDQ AAKDKSQDWE KHFLDASASK 250 260 270 280 290 300 PSDWKGELDG DWQAAMLQKP PYQDGLKPEG IDKDVWLHQK MKNSYLTEYD LSEFENIGAV 310 320 330 340 350 360 GLELWQVRSG TIFDNFLITD DEEYAENFGK ATWGETKGPE KEMDAIQAKE EVKKAQEEDE 370 380 DDMLMGRFRG RENSFKGFHR RNEF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC109750 mRNA. Translation: AAI09751.1.
IPI	IPI00731916.
RefSeq	NP_001033603.1. NM_001038514.2.
UniGene	Bt.54234.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000013175.
Proteomic databases
PRIDE	Q2TBR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	508555.
KEGG	bta:508555.
Organism-specific databases
CTD	125972.
Phylogenomic databases
eggNOG	NOG268919.
HOGENOM	HOG000192435.
HOVERGEN	HBG005407.
InParanoid	Q2TBR8.
KO	K10098.
OrthoDB	EOG41JZCD.
Enzyme and pathway databases
BioCyc	CATTLE:508555-MONOMER.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00014. ER_TARGET. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20868571.

Entry information

Entry name

CALR3_BOVIN

Accession

Primary (citable) accession number: Q2TBR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	January 24, 2006
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program