Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calreticulin-3

Gene

CALR3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. CALR3 capacity for calcium-binding may be absent or much lower than that of CALR. Required for sperm fertility (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109CarbohydrateBy similarity1
Binding sitei111CarbohydrateBy similarity1
Binding sitei128CarbohydrateBy similarity1
Binding sitei135CarbohydrateBy similarity1
Binding sitei303CarbohydrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

Lectin, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin-3
Alternative name(s):
Calsperin
Gene namesi
Name:CALR3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000028286720 – 384Calreticulin-3Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi105 ↔ 137By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ2TBR8.
PRIDEiQ2TBR8.

Interactioni

Subunit structurei

Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013175.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati191 – 2021-1Add BLAST12
Repeati209 – 2201-2Add BLAST12
Repeati222 – 2311-310
Repeati235 – 2461-4Add BLAST12
Repeati250 – 2602-1Add BLAST11
Repeati264 – 2722-29
Repeati274 – 2842-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 197N-domainAdd BLAST178
Regioni191 – 2464 X approximate repeatsAdd BLAST56
Regioni198 – 294P-domainAdd BLAST97
Regioni250 – 2843 X approximate repeatsAdd BLAST35
Regioni295 – 384C-domainAdd BLAST90

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi381 – 384Prevents secretion from ERSequence analysis4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi337 – 362Glu/Lys-richAdd BLAST26

Domaini

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity (By similarity).By similarity
The interaction with glycans occurs through a binding site in the globular lectin domain.By similarity
The zinc binding sites are localized to the N-domain.By similarity

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
HOGENOMiHOG000192435.
HOVERGENiHBG005407.
InParanoidiQ2TBR8.
KOiK10098.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TBR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAARVPLWA ICVRRVALAT VYFQEEFLDG ERWRNRWVHS TNDSQFGHFR
60 70 80 90 100
LSSGNFYGHK EKDKGLQTTQ NSRFYAISAR FKPFSNKGKT LIIQYTVKHE
110 120 130 140 150
QKMDCGGGYI KLFPADVDQK NLNGKSQYYI MFGPDICGFD IKTVHVILHF
160 170 180 190 200
KNQYHANKKS IRCKVDSFTH LYTLVLRPDL TYEVKIDGQS IESGSIEYDW
210 220 230 240 250
QLTSLKKMEK ASAEAEGWDQ AAKDKSQDWE KHFLDASASK PSDWKGELDG
260 270 280 290 300
DWQAAMLQKP PYQDGLKPEG IDKDVWLHQK MKNSYLTEYD LSEFENIGAV
310 320 330 340 350
GLELWQVRSG TIFDNFLITD DEEYAENFGK ATWGETKGPE KEMDAIQAKE
360 370 380
EVKKAQEEDE DDMLMGRFRG RENSFKGFHR RNEF
Length:384
Mass (Da):44,512
Last modified:January 24, 2006 - v1
Checksum:iBAE01C8F353718C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109750 mRNA. Translation: AAI09751.1.
RefSeqiNP_001033603.1. NM_001038514.2.
UniGeneiBt.54234.

Genome annotation databases

GeneIDi508555.
KEGGibta:508555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109750 mRNA. Translation: AAI09751.1.
RefSeqiNP_001033603.1. NM_001038514.2.
UniGeneiBt.54234.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013175.

Proteomic databases

PaxDbiQ2TBR8.
PRIDEiQ2TBR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi508555.
KEGGibta:508555.

Organism-specific databases

CTDi125972.

Phylogenomic databases

eggNOGiKOG0674. Eukaryota.
ENOG410XRR7. LUCA.
HOGENOMiHOG000192435.
HOVERGENiHBG005407.
InParanoidiQ2TBR8.
KOiK10098.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR013320. ConA-like_dom.
[Graphical view]
PANTHERiPTHR11073. PTHR11073. 1 hit.
PfamiPF00262. Calreticulin. 2 hits.
[Graphical view]
PIRSFiPIRSF002356. Calreticulin. 1 hit.
PRINTSiPR00626. CALRETICULIN.
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEiPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALR3_BOVIN
AccessioniPrimary (citable) accession number: Q2TBR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: January 24, 2006
Last modified: June 8, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.