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Q2TBR8 (CALR3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calreticulin-3
Alternative name(s):
Calsperin
Gene names
Name:CALR3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. CALR3 capacity for calcium-binding may be absent or much lower than that of CALR. Required for sperm fertility By similarity.

Subunit structure

Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity.

Subcellular location

Endoplasmic reticulum lumen By similarity.

Domain

Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity.

The interaction with glycans occurs through a binding site in the globular lectin domain By similarity.

The zinc binding sites are localized to the N-domain By similarity.

Sequence similarities

Belongs to the calreticulin family.

Ontologies

Keywords
   Biological processDifferentiation
Spermatogenesis
   Cellular componentEndoplasmic reticulum
   DomainRepeat
Signal
   LigandLectin
Metal-binding
Zinc
   Molecular functionChaperone
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 384365Calreticulin-3
PRO_0000282867

Regions

Repeat191 – 202121-1
Repeat209 – 220121-2
Repeat222 – 231101-3
Repeat235 – 246121-4
Repeat250 – 260112-1
Repeat264 – 27292-2
Repeat274 – 284112-3
Region20 – 197178N-domain
Region191 – 246564 X approximate repeats
Region198 – 29497P-domain
Region250 – 284353 X approximate repeats
Region295 – 38490C-domain
Motif381 – 3844Prevents secretion from ER Potential
Compositional bias337 – 36226Glu/Lys-rich

Sites

Binding site1091Carbohydrate By similarity
Binding site1111Carbohydrate By similarity
Binding site1281Carbohydrate By similarity
Binding site1351Carbohydrate By similarity
Binding site3031Carbohydrate By similarity

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Potential
Disulfide bond105 ↔ 137 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2TBR8 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: BAE01C8F353718C2

FASTA38444,512
        10         20         30         40         50         60 
MAAARVPLWA ICVRRVALAT VYFQEEFLDG ERWRNRWVHS TNDSQFGHFR LSSGNFYGHK 

        70         80         90        100        110        120 
EKDKGLQTTQ NSRFYAISAR FKPFSNKGKT LIIQYTVKHE QKMDCGGGYI KLFPADVDQK 

       130        140        150        160        170        180 
NLNGKSQYYI MFGPDICGFD IKTVHVILHF KNQYHANKKS IRCKVDSFTH LYTLVLRPDL 

       190        200        210        220        230        240 
TYEVKIDGQS IESGSIEYDW QLTSLKKMEK ASAEAEGWDQ AAKDKSQDWE KHFLDASASK 

       250        260        270        280        290        300 
PSDWKGELDG DWQAAMLQKP PYQDGLKPEG IDKDVWLHQK MKNSYLTEYD LSEFENIGAV 

       310        320        330        340        350        360 
GLELWQVRSG TIFDNFLITD DEEYAENFGK ATWGETKGPE KEMDAIQAKE EVKKAQEEDE 

       370        380 
DDMLMGRFRG RENSFKGFHR RNEF 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC109750 mRNA. Translation: AAI09751.1.
RefSeqNP_001033603.1. NM_001038514.2.
UniGeneBt.54234.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000013175.

Proteomic databases

PRIDEQ2TBR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID508555.
KEGGbta:508555.

Organism-specific databases

CTD125972.

Phylogenomic databases

eggNOGNOG268919.
HOGENOMHOG000192435.
HOVERGENHBG005407.
InParanoidQ2TBR8.
KOK10098.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERPTHR11073. PTHR11073. 1 hit.
PfamPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFPIRSF002356. Calreticulin. 1 hit.
PRINTSPR00626. CALRETICULIN.
SUPFAMSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20868571.

Entry information

Entry nameCALR3_BOVIN
AccessionPrimary (citable) accession number: Q2TBR8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: January 24, 2006
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families