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Protein

Guanidinoacetate N-methyltransferase

Gene

GAMT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.PROSITE-ProRule annotation

Pathwayi: creatine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes creatine from L-arginine and glycine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (GATM)
  2. Guanidinoacetate N-methyltransferase (GAMT)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei42SubstratePROSITE-ProRule annotation1
Binding sitei46SubstratePROSITE-ProRule annotation1
Binding sitei50S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei135S-adenosyl-L-methionine and substratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-BTA-71288 Creatine metabolism
UniPathwayiUPA00104; UER00580

Names & Taxonomyi

Protein namesi
Recommended name:
Guanidinoacetate N-methyltransferase (EC:2.1.1.2)
Gene namesi
Name:GAMT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:29243 GAMT

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002289642 – 236Guanidinoacetate N-methyltransferaseAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2TBQ3
PeptideAtlasiQ2TBQ3
PRIDEiQ2TBQ3

Expressioni

Gene expression databases

BgeeiENSBTAG00000004112

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005375

Structurei

3D structure databases

ProteinModelPortaliQ2TBQ3
SMRiQ2TBQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 236RMT2PROSITE-ProRule annotationAdd BLAST224

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 74S-adenosyl-L-methionine bindingPROSITE-ProRule annotation6
Regioni90 – 92S-adenosyl-L-methioninePROSITE-ProRule annotation3
Regioni117 – 118S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2
Regioni171 – 172Substrate bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1709 Eukaryota
ENOG4110T2S LUCA
GeneTreeiENSGT00390000018061
HOGENOMiHOG000010290
HOVERGENiHBG005801
InParanoidiQ2TBQ3
KOiK00542
OMAiRYYAFPQ
OrthoDBiEOG091G0EPW
TreeFamiTF328555

Family and domain databases

InterProiView protein in InterPro
IPR016550 GuanidinoAc_N-MeTrfase
IPR026480 RMT2_dom
IPR029063 SAM-dependent_MTases
PIRSFiPIRSF009285 GAMT, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51559 SAM_RMT2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TBQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAATPIF APGENCSPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM
60 70 80 90 100
HALAAAAASR GGRVLEVGFG MAIAATKVQE APIEEHWIIE CNEGVFQRLQ
110 120 130 140 150
DWALQQPHKV VPLKGLWEEV APTLPDSHFD GILYDTYPLS EETWHTHQFN
160 170 180 190 200
FIRDHAFRLL KPGGVLTYCN LTSWGELMKT KYSDITTMFE ETQVPALLEA
210 220 230
GFRRDNIRTQ VMELVPPANC RYYAFPRMIT PLVTKH
Length:236
Mass (Da):26,610
Last modified:January 24, 2006 - v1
Checksum:i18B61B459C934179
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109825 mRNA Translation: AAI09826.1
RefSeqiNP_001033633.1, NM_001038544.2
UniGeneiBt.13865

Genome annotation databases

EnsembliENSBTAT00000005375; ENSBTAP00000005375; ENSBTAG00000004112
GeneIDi515270
KEGGibta:515270

Similar proteinsi

Entry informationi

Entry nameiGAMT_BOVIN
AccessioniPrimary (citable) accession number: Q2TBQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: January 24, 2006
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health