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Protein

Guanidinoacetate N-methyltransferase

Gene

GAMT

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Converts guanidinoacetate to creatine, using S-adenosylmethionine as the methyl donor. Important in nervous system development.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.PROSITE-ProRule annotation

Pathwayi: creatine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes creatine from L-arginine and glycine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (GATM)
  2. Guanidinoacetate N-methyltransferase (GAMT)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei42SubstratePROSITE-ProRule annotation1
Binding sitei46SubstratePROSITE-ProRule annotation1
Binding sitei50S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei135S-adenosyl-L-methionine and substratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-BTA-71288. Creatine metabolism.
UniPathwayiUPA00104; UER00580.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanidinoacetate N-methyltransferase (EC:2.1.1.2)
Gene namesi
Name:GAMT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002289642 – 236Guanidinoacetate N-methyltransferaseAdd BLAST235

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2TBQ3.
PeptideAtlasiQ2TBQ3.
PRIDEiQ2TBQ3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004112.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005375.

Structurei

3D structure databases

ProteinModelPortaliQ2TBQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 236RMT2PROSITE-ProRule annotationAdd BLAST224

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 74S-adenosyl-L-methionine bindingPROSITE-ProRule annotation6
Regioni90 – 92S-adenosyl-L-methioninePROSITE-ProRule annotation3
Regioni117 – 118S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2
Regioni171 – 172Substrate bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.PROSITE-ProRule annotation
Contains 1 RMT2 (arginine N-methyltransferase 2-like) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1709. Eukaryota.
ENOG4110T2S. LUCA.
GeneTreeiENSGT00390000018061.
HOGENOMiHOG000010290.
HOVERGENiHBG005801.
InParanoidiQ2TBQ3.
KOiK00542.
OMAiRYYAFPQ.
OrthoDBiEOG091G0EPW.
TreeFamiTF328555.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016550. GuanidinoAc_N-MeTrfase.
IPR026480. RMT2_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF009285. GAMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51559. SAM_RMT2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TBQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPAATPIF APGENCSPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM
60 70 80 90 100
HALAAAAASR GGRVLEVGFG MAIAATKVQE APIEEHWIIE CNEGVFQRLQ
110 120 130 140 150
DWALQQPHKV VPLKGLWEEV APTLPDSHFD GILYDTYPLS EETWHTHQFN
160 170 180 190 200
FIRDHAFRLL KPGGVLTYCN LTSWGELMKT KYSDITTMFE ETQVPALLEA
210 220 230
GFRRDNIRTQ VMELVPPANC RYYAFPRMIT PLVTKH
Length:236
Mass (Da):26,610
Last modified:January 24, 2006 - v1
Checksum:i18B61B459C934179
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109825 mRNA. Translation: AAI09826.1.
RefSeqiNP_001033633.1. NM_001038544.2.
UniGeneiBt.13865.

Genome annotation databases

EnsembliENSBTAT00000005375; ENSBTAP00000005375; ENSBTAG00000004112.
GeneIDi515270.
KEGGibta:515270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109825 mRNA. Translation: AAI09826.1.
RefSeqiNP_001033633.1. NM_001038544.2.
UniGeneiBt.13865.

3D structure databases

ProteinModelPortaliQ2TBQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005375.

Proteomic databases

PaxDbiQ2TBQ3.
PeptideAtlasiQ2TBQ3.
PRIDEiQ2TBQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000005375; ENSBTAP00000005375; ENSBTAG00000004112.
GeneIDi515270.
KEGGibta:515270.

Organism-specific databases

CTDi2593.

Phylogenomic databases

eggNOGiKOG1709. Eukaryota.
ENOG4110T2S. LUCA.
GeneTreeiENSGT00390000018061.
HOGENOMiHOG000010290.
HOVERGENiHBG005801.
InParanoidiQ2TBQ3.
KOiK00542.
OMAiRYYAFPQ.
OrthoDBiEOG091G0EPW.
TreeFamiTF328555.

Enzyme and pathway databases

UniPathwayiUPA00104; UER00580.
ReactomeiR-BTA-71288. Creatine metabolism.

Gene expression databases

BgeeiENSBTAG00000004112.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016550. GuanidinoAc_N-MeTrfase.
IPR026480. RMT2_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF009285. GAMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51559. SAM_RMT2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAMT_BOVIN
AccessioniPrimary (citable) accession number: Q2TBQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.