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Protein

Mitochondrial dimethyladenosine transferase 1

Gene

TFB1M

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei38S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei63S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionineBy similarity1
Binding sitei111S-adenosyl-L-methionineBy similarity1
Binding sitei141S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Methyltransferase, RNA-binding, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dimethyladenosine transferase 1 (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 1
Mitochondrial transcription factor B1
Short name:
mtTFB1
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
Gene namesi
Name:TFB1M
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 9

Organism-specific databases

VGNCiVGNC:35782 TFB1M

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionSequence analysisAdd BLAST27
ChainiPRO_000027317028 – 341Mitochondrial dimethyladenosine transferase 1Add BLAST314

Proteomic databases

PaxDbiQ2TBQ0
PRIDEiQ2TBQ0

Expressioni

Gene expression databases

BgeeiENSBTAG00000031895
ExpressionAtlasiQ2TBQ0 baseline and differential

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042644

Structurei

3D structure databases

ProteinModelPortaliQ2TBQ0
SMRiQ2TBQ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38S-adenosyl-L-methionine bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0821 Eukaryota
COG0030 LUCA
GeneTreeiENSGT00530000063389
HOGENOMiHOG000227961
HOVERGENiHBG082484
InParanoidiQ2TBQ0
KOiK15266
OMAiSVMSQIW
OrthoDBiEOG091G0ALA
TreeFamiTF300798

Family and domain databases

Gene3Di1.10.8.100, 1 hit
HAMAPiMF_00607 16SrRNA_methyltr_A, 1 hit
InterProiView protein in InterPro
IPR001737 KsgA/Erm
IPR023165 rRNA_Ade_diMease-like
IPR020596 rRNA_Ade_Mease_Trfase_CS
IPR020598 rRNA_Ade_methylase_Trfase_N
IPR011530 rRNA_adenine_dimethylase
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11727 PTHR11727, 1 hit
PfamiView protein in Pfam
PF00398 RrnaAD, 1 hit
SMARTiView protein in SMART
SM00650 rADc, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00755 ksgA, 1 hit
PROSITEiView protein in PROSITE
PS01131 RRNA_A_DIMETH, 1 hit
PS51689 SAM_RNA_A_N6_MT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2TBQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSRTLGTF RLPPLPTIRE IIKLFRLQAV KQLSQNFLLD LRLTDKIVRK
60 70 80 90 100
AGNLTNAYVY EVGPGPGGIT RSILNAGVAE LLVVEKDSRF IPGLQMLSDA
110 120 130 140 150
APGKLRIVHG DVLTFKIERA FPESLKRQWE DDPPNVHIIG NLPFSVSTPL
160 170 180 190 200
IIKWLENVSQ RNGPFAYGRT RMMLTFQKEV AERLTATTGS KQRSRLSIMA
210 220 230 240 250
QYLCDVQHIL TIPGQAFVPK PEVDSGVVHF TPLTRPRIKQ PFKLVEKVVQ
260 270 280 290 300
NAFQFRRKYC HRGLGMLFPE ARRLESTGKL LELADVDPTL RPTQLTVSHF
310 320 330 340
KSLCDVYRKM CDEDPHLFAY NFREELRQKS KKEDDKQSCR L
Length:341
Mass (Da):38,883
Last modified:January 23, 2007 - v2
Checksum:i978780B2A36AF2C1
GO
Isoform 2 (identifier: Q2TBQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-226: VDSG → NVIP
     227-341: Missing.

Note: No experimental confirmation available.
Show »
Length:226
Mass (Da):25,271
Checksum:i59970ED0369AFF25
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022489223 – 226VDSG → NVIP in isoform 2. 1 Publication4
Alternative sequenceiVSP_022490227 – 341Missing in isoform 2. 1 PublicationAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109841 mRNA Translation: AAI09842.1
DT811342 mRNA No translation available.
RefSeqiNP_001070364.1, NM_001076896.2 [Q2TBQ0-2]
NP_001108082.1, NM_001114610.1 [Q2TBQ0-1]
UniGeneiBt.56407

Genome annotation databases

EnsembliENSBTAT00000045233; ENSBTAP00000042642; ENSBTAG00000031895 [Q2TBQ0-2]
ENSBTAT00000045235; ENSBTAP00000042644; ENSBTAG00000031895 [Q2TBQ0-1]
GeneIDi533438
KEGGibta:533438

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTFB1M_BOVIN
AccessioniPrimary (citable) accession number: Q2TBQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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