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Protein

Mitochondrial dimethyladenosine transferase 1

Gene

TFB1M

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei38S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei63S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionineBy similarity1
Binding sitei111S-adenosyl-L-methionineBy similarity1
Binding sitei141S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-BTA-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial dimethyladenosine transferase 1 (EC:2.1.1.-)
Alternative name(s):
Mitochondrial 12S rRNA dimethylase 1
Mitochondrial transcription factor B1
Short name:
mtTFB1
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1
Gene namesi
Name:TFB1M
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 9

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionSequence analysisAdd BLAST27
ChainiPRO_000027317028 – 341Mitochondrial dimethyladenosine transferase 1Add BLAST314

Proteomic databases

PaxDbiQ2TBQ0.
PRIDEiQ2TBQ0.

Expressioni

Gene expression databases

BgeeiENSBTAG00000031895.
ExpressionAtlasiQ2TBQ0. baseline and differential.

Interactioni

Subunit structurei

Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042644.

Structurei

3D structure databases

ProteinModelPortaliQ2TBQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38S-adenosyl-L-methionine bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0821. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227961.
HOVERGENiHBG082484.
InParanoidiQ2TBQ0.
KOiK15266.
OMAiFQFRRKH.
OrthoDBiEOG091G0ALA.
TreeFamiTF300798.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2TBQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSRTLGTF RLPPLPTIRE IIKLFRLQAV KQLSQNFLLD LRLTDKIVRK
60 70 80 90 100
AGNLTNAYVY EVGPGPGGIT RSILNAGVAE LLVVEKDSRF IPGLQMLSDA
110 120 130 140 150
APGKLRIVHG DVLTFKIERA FPESLKRQWE DDPPNVHIIG NLPFSVSTPL
160 170 180 190 200
IIKWLENVSQ RNGPFAYGRT RMMLTFQKEV AERLTATTGS KQRSRLSIMA
210 220 230 240 250
QYLCDVQHIL TIPGQAFVPK PEVDSGVVHF TPLTRPRIKQ PFKLVEKVVQ
260 270 280 290 300
NAFQFRRKYC HRGLGMLFPE ARRLESTGKL LELADVDPTL RPTQLTVSHF
310 320 330 340
KSLCDVYRKM CDEDPHLFAY NFREELRQKS KKEDDKQSCR L
Length:341
Mass (Da):38,883
Last modified:January 23, 2007 - v2
Checksum:i978780B2A36AF2C1
GO
Isoform 2 (identifier: Q2TBQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-226: VDSG → NVIP
     227-341: Missing.

Note: No experimental confirmation available.
Show »
Length:226
Mass (Da):25,271
Checksum:i59970ED0369AFF25
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022489223 – 226VDSG → NVIP in isoform 2. 1 Publication4
Alternative sequenceiVSP_022490227 – 341Missing in isoform 2. 1 PublicationAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109841 mRNA. Translation: AAI09842.1.
DT811342 mRNA. No translation available.
RefSeqiNP_001070364.1. NM_001076896.2. [Q2TBQ0-2]
NP_001108082.1. NM_001114610.1. [Q2TBQ0-1]
UniGeneiBt.56407.

Genome annotation databases

EnsembliENSBTAT00000045233; ENSBTAP00000042642; ENSBTAG00000031895. [Q2TBQ0-2]
ENSBTAT00000045235; ENSBTAP00000042644; ENSBTAG00000031895. [Q2TBQ0-1]
GeneIDi533438.
KEGGibta:533438.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC109841 mRNA. Translation: AAI09842.1.
DT811342 mRNA. No translation available.
RefSeqiNP_001070364.1. NM_001076896.2. [Q2TBQ0-2]
NP_001108082.1. NM_001114610.1. [Q2TBQ0-1]
UniGeneiBt.56407.

3D structure databases

ProteinModelPortaliQ2TBQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000042644.

Proteomic databases

PaxDbiQ2TBQ0.
PRIDEiQ2TBQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000045233; ENSBTAP00000042642; ENSBTAG00000031895. [Q2TBQ0-2]
ENSBTAT00000045235; ENSBTAP00000042644; ENSBTAG00000031895. [Q2TBQ0-1]
GeneIDi533438.
KEGGibta:533438.

Organism-specific databases

CTDi51106.

Phylogenomic databases

eggNOGiKOG0821. Eukaryota.
COG0030. LUCA.
GeneTreeiENSGT00530000063389.
HOGENOMiHOG000227961.
HOVERGENiHBG082484.
InParanoidiQ2TBQ0.
KOiK15266.
OMAiFQFRRKH.
OrthoDBiEOG091G0ALA.
TreeFamiTF300798.

Enzyme and pathway databases

ReactomeiR-BTA-2151201. Transcriptional activation of mitochondrial biogenesis.

Gene expression databases

BgeeiENSBTAG00000031895.
ExpressionAtlasiQ2TBQ0. baseline and differential.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFB1M_BOVIN
AccessioniPrimary (citable) accession number: Q2TBQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.