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Q2TBJ5 (ATG12_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-like protein ATG12
Alternative name(s):
Autophagy-related protein 12
Short name=APG12-like
Gene names
Name:Atg12
Synonyms:Apg12l
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length141 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus By similarity.

Subunit structure

Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1 By similarity.

Subcellular location

Cytoplasm By similarity. Preautophagosomal structure membrane; Peripheral membrane protein By similarity. Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane By similarity.

Induction

Activated in retinal ganglion cells (RGCs) following optic nerve transection. Also induced under starvation conditions, through the action of the foxo1 and foxo3 transcription factors. Ref.2 Ref.3

Domain

Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage By similarity.

Post-translational modification

Acetylated by EP300 By similarity.

Sequence similarities

Belongs to the ATG12 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 141141Ubiquitin-like protein ATG12
PRO_0000233273

Amino acid modifications

Cross-link141Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2TBJ5 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 938B3F50F6454CB6

FASTA14115,284
        10         20         30         40         50         60 
MAEDPEAVLQ LPAAPAAAAG ESLLELSPET AIPEPPSSVA VSPGTEEPPG DTKKKIDILL 

        70         80         90        100        110        120 
KAVGDTPIMK TKKWAVERTR TVQALIDFIR KFLRLLASEQ LFIYVNQSFA PSPDQEVGTL 

       130        140 
YECFGSDGKL VLHYCKSQAW G 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney and Placenta.
[2]"Activation of autophagy in retinal ganglion cells."
Kim S.H., Munemasa Y., Kwong J.M., Ahn J.H., Mareninov S., Gordon L.K., Caprioli J., Piri N.
J. Neurosci. Res. 86:2943-2951(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[3]"FoxO transcription factors promote autophagy in cardiomyocytes."
Sengupta A., Molkentin J.D., Yutzey K.E.
J. Biol. Chem. 284:28319-28331(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC087139 mRNA. Translation: AAH87139.1.
BC110056 mRNA. Translation: AAI10057.1.
RefSeqNP_001033584.1. NM_001038495.1.
UniGeneRn.6224.

3D structure databases

ProteinModelPortalQ2TBJ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid262598. 1 interaction.
STRING10116.ENSRNOP00000000170.

Proteomic databases

PaxDbQ2TBJ5.
PRIDEQ2TBJ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000170; ENSRNOP00000000170; ENSRNOG00000000157.
GeneID361321.
KEGGrno:361321.
UCSCRGD:1306306. rat.

Organism-specific databases

CTD9140.
RGD1306306. Atg12.

Phylogenomic databases

eggNOGNOG236266.
GeneTreeENSGT00390000016654.
HOGENOMHOG000234863.
HOVERGENHBG080875.
InParanoidQ2TBJ5.
KOK08336.
OMAPWRKARP.
OrthoDBEOG7XPZ7X.
PhylomeDBQ2TBJ5.
TreeFamTF325131.

Gene expression databases

GenevestigatorQ2TBJ5.

Family and domain databases

InterProIPR007242. Atg12.
[Graphical view]
PANTHERPTHR13385. PTHR13385. 1 hit.
PfamPF04110. APG12. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio675928.
PROQ2TBJ5.

Entry information

Entry nameATG12_RAT
AccessionPrimary (citable) accession number: Q2TBJ5
Secondary accession number(s): Q5M9F9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: January 24, 2006
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families