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Q2TBJ5

- ATG12_RAT

UniProt

Q2TBJ5 - ATG12_RAT

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Protein
Ubiquitin-like protein ATG12
Gene
Atg12, Apg12l
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus By similarity.

GO - Molecular functioni

  1. protein binding Source: RGD

GO - Biological processi

  1. autophagic vacuole assembly Source: Ensembl
  2. autophagy Source: RGD
  3. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Immunity, Innate immunity, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_198997. Negative regulators of RIG-I/MDA5 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein ATG12
Alternative name(s):
Autophagy-related protein 12
Short name:
APG12-like
Gene namesi
Name:Atg12
Synonyms:Apg12l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 18

Organism-specific databases

RGDi1306306. Atg12.

Subcellular locationi

Cytoplasm By similarity. Preautophagosomal structure membrane; Peripheral membrane protein By similarity
Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane By similarity.

GO - Cellular componenti

  1. membrane Source: RGD
  2. pre-autophagosomal structure membrane Source: UniProtKB-SubCell
  3. protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141Ubiquitin-like protein ATG12
PRO_0000233273Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki141 – 141Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein) By similarity

Post-translational modificationi

Acetylated by EP300 By similarity.

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

PaxDbiQ2TBJ5.
PRIDEiQ2TBJ5.

Expressioni

Inductioni

Activated in retinal ganglion cells (RGCs) following optic nerve transection. Also induced under starvation conditions, through the action of the foxo1 and foxo3 transcription factors.2 Publications

Gene expression databases

GenevestigatoriQ2TBJ5.

Interactioni

Subunit structurei

Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1 By similarity.

Protein-protein interaction databases

BioGridi262598. 1 interaction.
STRINGi10116.ENSRNOP00000000170.

Structurei

3D structure databases

ProteinModelPortaliQ2TBJ5.

Family & Domainsi

Domaini

Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage By similarity.

Sequence similaritiesi

Belongs to the ATG12 family.

Phylogenomic databases

eggNOGiNOG236266.
GeneTreeiENSGT00390000016654.
HOGENOMiHOG000234863.
HOVERGENiHBG080875.
InParanoidiQ2TBJ5.
KOiK08336.
OMAiPWRKARP.
OrthoDBiEOG7XPZ7X.
PhylomeDBiQ2TBJ5.
TreeFamiTF325131.

Family and domain databases

InterProiIPR007242. Atg12.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13385. PTHR13385. 1 hit.
PfamiPF04110. APG12. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2TBJ5-1 [UniParc]FASTAAdd to Basket

« Hide

MAEDPEAVLQ LPAAPAAAAG ESLLELSPET AIPEPPSSVA VSPGTEEPPG    50
DTKKKIDILL KAVGDTPIMK TKKWAVERTR TVQALIDFIR KFLRLLASEQ 100
LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G 141
Length:141
Mass (Da):15,284
Last modified:January 24, 2006 - v1
Checksum:i938B3F50F6454CB6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC087139 mRNA. Translation: AAH87139.1.
BC110056 mRNA. Translation: AAI10057.1.
RefSeqiNP_001033584.1. NM_001038495.1.
UniGeneiRn.6224.

Genome annotation databases

EnsembliENSRNOT00000000170; ENSRNOP00000000170; ENSRNOG00000000157.
GeneIDi361321.
KEGGirno:361321.
UCSCiRGD:1306306. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC087139 mRNA. Translation: AAH87139.1 .
BC110056 mRNA. Translation: AAI10057.1 .
RefSeqi NP_001033584.1. NM_001038495.1.
UniGenei Rn.6224.

3D structure databases

ProteinModelPortali Q2TBJ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 262598. 1 interaction.
STRINGi 10116.ENSRNOP00000000170.

Proteomic databases

PaxDbi Q2TBJ5.
PRIDEi Q2TBJ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000000170 ; ENSRNOP00000000170 ; ENSRNOG00000000157 .
GeneIDi 361321.
KEGGi rno:361321.
UCSCi RGD:1306306. rat.

Organism-specific databases

CTDi 9140.
RGDi 1306306. Atg12.

Phylogenomic databases

eggNOGi NOG236266.
GeneTreei ENSGT00390000016654.
HOGENOMi HOG000234863.
HOVERGENi HBG080875.
InParanoidi Q2TBJ5.
KOi K08336.
OMAi PWRKARP.
OrthoDBi EOG7XPZ7X.
PhylomeDBi Q2TBJ5.
TreeFami TF325131.

Enzyme and pathway databases

Reactomei REACT_198997. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

NextBioi 675928.
PROi Q2TBJ5.

Gene expression databases

Genevestigatori Q2TBJ5.

Family and domain databases

InterProi IPR007242. Atg12.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR13385. PTHR13385. 1 hit.
Pfami PF04110. APG12. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Placenta.
  2. Cited for: INDUCTION.
  3. "FoxO transcription factors promote autophagy in cardiomyocytes."
    Sengupta A., Molkentin J.D., Yutzey K.E.
    J. Biol. Chem. 284:28319-28331(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiATG12_RAT
AccessioniPrimary (citable) accession number: Q2TBJ5
Secondary accession number(s): Q5M9F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: January 24, 2006
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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