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Protein

Ubiquitin-like protein ATG12

Gene

Atg12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Immunity, Innate immunity, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_316967. Negative regulators of RIG-I/MDA5 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein ATG12
Alternative name(s):
Autophagy-related protein 12
Short name:
APG12-like
Gene namesi
Name:Atg12
Synonyms:Apg12l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1306306. Atg12.

Subcellular locationi

GO - Cellular componenti

  • Atg12-Atg5-Atg16 complex Source: GO_Central
  • membrane Source: RGD
  • pre-autophagosomal structure membrane Source: GO_Central
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141Ubiquitin-like protein ATG12PRO_0000233273Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki141 – 141Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)By similarity

Post-translational modificationi

Acetylated by EP300.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

PaxDbiQ2TBJ5.
PRIDEiQ2TBJ5.

Expressioni

Inductioni

Activated in retinal ganglion cells (RGCs) following optic nerve transection. Also induced under starvation conditions, through the action of the foxo1 and foxo3 transcription factors.2 Publications

Gene expression databases

GenevisibleiQ2TBJ5. RN.

Interactioni

Subunit structurei

Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi262598. 1 interaction.
STRINGi10116.ENSRNOP00000000170.

Structurei

3D structure databases

ProteinModelPortaliQ2TBJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.By similarity

Sequence similaritiesi

Belongs to the ATG12 family.Curated

Phylogenomic databases

eggNOGiNOG236266.
GeneTreeiENSGT00390000016654.
HOGENOMiHOG000234863.
HOVERGENiHBG080875.
InParanoidiQ2TBJ5.
KOiK08336.
OMAiPWRKARP.
OrthoDBiEOG7XPZ7X.
PhylomeDBiQ2TBJ5.
TreeFamiTF325131.

Family and domain databases

InterProiIPR007242. Atg12.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13385. PTHR13385. 1 hit.
PfamiPF04110. APG12. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2TBJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEDPEAVLQ LPAAPAAAAG ESLLELSPET AIPEPPSSVA VSPGTEEPPG
60 70 80 90 100
DTKKKIDILL KAVGDTPIMK TKKWAVERTR TVQALIDFIR KFLRLLASEQ
110 120 130 140
LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G
Length:141
Mass (Da):15,284
Last modified:January 24, 2006 - v1
Checksum:i938B3F50F6454CB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087139 mRNA. Translation: AAH87139.1.
BC110056 mRNA. Translation: AAI10057.1.
RefSeqiNP_001033584.1. NM_001038495.1.
UniGeneiRn.6224.

Genome annotation databases

EnsembliENSRNOT00000000170; ENSRNOP00000000170; ENSRNOG00000000157.
GeneIDi361321.
KEGGirno:361321.
UCSCiRGD:1306306. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087139 mRNA. Translation: AAH87139.1.
BC110056 mRNA. Translation: AAI10057.1.
RefSeqiNP_001033584.1. NM_001038495.1.
UniGeneiRn.6224.

3D structure databases

ProteinModelPortaliQ2TBJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262598. 1 interaction.
STRINGi10116.ENSRNOP00000000170.

Proteomic databases

PaxDbiQ2TBJ5.
PRIDEiQ2TBJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000170; ENSRNOP00000000170; ENSRNOG00000000157.
GeneIDi361321.
KEGGirno:361321.
UCSCiRGD:1306306. rat.

Organism-specific databases

CTDi9140.
RGDi1306306. Atg12.

Phylogenomic databases

eggNOGiNOG236266.
GeneTreeiENSGT00390000016654.
HOGENOMiHOG000234863.
HOVERGENiHBG080875.
InParanoidiQ2TBJ5.
KOiK08336.
OMAiPWRKARP.
OrthoDBiEOG7XPZ7X.
PhylomeDBiQ2TBJ5.
TreeFamiTF325131.

Enzyme and pathway databases

ReactomeiREACT_316967. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

NextBioi675928.
PROiQ2TBJ5.

Gene expression databases

GenevisibleiQ2TBJ5. RN.

Family and domain databases

InterProiIPR007242. Atg12.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13385. PTHR13385. 1 hit.
PfamiPF04110. APG12. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Placenta.
  2. Cited for: INDUCTION.
  3. "FoxO transcription factors promote autophagy in cardiomyocytes."
    Sengupta A., Molkentin J.D., Yutzey K.E.
    J. Biol. Chem. 284:28319-28331(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiATG12_RAT
AccessioniPrimary (citable) accession number: Q2TBJ5
Secondary accession number(s): Q5M9F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: January 24, 2006
Last modified: July 22, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.