Q2TBJ5 (ATG12_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like protein ATG12

Alternative name(s):
Autophagy-related protein 12
Short name=APG12-like

Gene names

Name:	Atg12
Synonyms:	Apg12l

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5 By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage By similarity.
Sequence similarities	Belongs to the ATG12 family.

Ontologies

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm
PTM	Isopeptide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from electronic annotation. Source: Compara autophagy Inferred from mutant phenotype PubMed 18059433. Source: RGD
Cellular_component	membrane Inferred from direct assay PubMed 18059433. Source: RGD pre-autophagosomal structure membrane Inferred from electronic annotation. Source: Compara protein complex Inferred from direct assay PubMed 18059433. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	Ubiquitin-like protein ATG12		PRO_0000233273
Amino acid modifications
	Cross-link	141		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TBJ5 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 938B3F50F6454CB6	FASTA	141	15,284
	10 20 30 40 50 60 MAEDPEAVLQ LPAAPAAAAG ESLLELSPET AIPEPPSSVA VSPGTEEPPG DTKKKIDILL 70 80 90 100 110 120 KAVGDTPIMK TKKWAVERTR TVQALIDFIR KFLRLLASEQ LFIYVNQSFA PSPDQEVGTL 130 140 YECFGSDGKL VLHYCKSQAW G

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC087139 mRNA. Translation: AAH87139.1. BC110056 mRNA. Translation: AAI10057.1.
IPI	IPI00211923.
RefSeq	NP_001033584.1. NM_001038495.1.
UniGene	Rn.6224.
3D structure databases
ProteinModelPortal	Q2TBJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000000170.
Proteomic databases
PaxDb	Q2TBJ5.
PRIDE	Q2TBJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000000170; ENSRNOP00000000170; ENSRNOG00000000157.
GeneID	361321.
KEGG	rno:361321.
UCSC	RGD:1306306. rat.
Organism-specific databases
CTD	9140.
RGD	1306306. Atg12.
Phylogenomic databases
eggNOG	NOG236266.
GeneTree	ENSGT00390000016654.
HOGENOM	HOG000234863.
HOVERGEN	HBG080875.
InParanoid	Q2TBJ5.
KO	K08336.
OMA	PWRKARP.
OrthoDB	EOG4MGS8V.
Gene expression databases
Genevestigator	Q2TBJ5.
GermOnline	ENSRNOG00000000157. Rattus norvegicus.
Family and domain databases
InterPro	IPR007242. Atg12. [Graphical view]
PANTHER	PTHR13385. PTHR13385. 1 hit.
Pfam	PF04110. APG12. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	675928.

Entry information

Entry name

ATG12_RAT

Accession

Primary (citable) accession number: Q2TBJ5
Secondary accession number(s): Q5M9F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program