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Q2TBJ5

- ATG12_RAT

UniProt

Q2TBJ5 - ATG12_RAT

Protein

Ubiquitin-like protein ATG12

Gene

Atg12

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus By similarity.By similarity

    GO - Molecular functioni

    1. Atg8 ligase activity Source: RefGenome
    2. protein binding Source: RGD

    GO - Biological processi

    1. autophagic vacuole assembly Source: RefGenome
    2. autophagy Source: RGD
    3. cellular response to nitrogen starvation Source: RefGenome
    4. C-terminal protein lipidation Source: RefGenome
    5. innate immune response Source: UniProtKB-KW
    6. mitochondrion degradation Source: RefGenome
    7. nucleophagy Source: RefGenome

    Keywords - Biological processi

    Autophagy, Immunity, Innate immunity, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_198997. Negative regulators of RIG-I/MDA5 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-like protein ATG12
    Alternative name(s):
    Autophagy-related protein 12
    Short name:
    APG12-like
    Gene namesi
    Name:Atg12
    Synonyms:Apg12l
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 18

    Organism-specific databases

    RGDi1306306. Atg12.

    Subcellular locationi

    Cytoplasm By similarity. Preautophagosomal structure membrane By similarity; Peripheral membrane protein By similarity
    Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.By similarity

    GO - Cellular componenti

    1. Atg12-Atg5-Atg16 complex Source: RefGenome
    2. membrane Source: RGD
    3. pre-autophagosomal structure membrane Source: RefGenome
    4. protein complex Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 141141Ubiquitin-like protein ATG12PRO_0000233273Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki141 – 141Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)By similarity

    Post-translational modificationi

    Acetylated by EP300.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond

    Proteomic databases

    PaxDbiQ2TBJ5.
    PRIDEiQ2TBJ5.

    Expressioni

    Inductioni

    Activated in retinal ganglion cells (RGCs) following optic nerve transection. Also induced under starvation conditions, through the action of the foxo1 and foxo3 transcription factors.2 Publications

    Gene expression databases

    GenevestigatoriQ2TBJ5.

    Interactioni

    Subunit structurei

    Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi262598. 1 interaction.
    STRINGi10116.ENSRNOP00000000170.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2TBJ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.By similarity

    Sequence similaritiesi

    Belongs to the ATG12 family.Curated

    Phylogenomic databases

    eggNOGiNOG236266.
    GeneTreeiENSGT00390000016654.
    HOGENOMiHOG000234863.
    HOVERGENiHBG080875.
    InParanoidiQ2TBJ5.
    KOiK08336.
    OMAiPWRKARP.
    OrthoDBiEOG7XPZ7X.
    PhylomeDBiQ2TBJ5.
    TreeFamiTF325131.

    Family and domain databases

    InterProiIPR007242. Atg12.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR13385. PTHR13385. 1 hit.
    PfamiPF04110. APG12. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2TBJ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEDPEAVLQ LPAAPAAAAG ESLLELSPET AIPEPPSSVA VSPGTEEPPG    50
    DTKKKIDILL KAVGDTPIMK TKKWAVERTR TVQALIDFIR KFLRLLASEQ 100
    LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G 141
    Length:141
    Mass (Da):15,284
    Last modified:January 24, 2006 - v1
    Checksum:i938B3F50F6454CB6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC087139 mRNA. Translation: AAH87139.1.
    BC110056 mRNA. Translation: AAI10057.1.
    RefSeqiNP_001033584.1. NM_001038495.1.
    UniGeneiRn.6224.

    Genome annotation databases

    EnsembliENSRNOT00000000170; ENSRNOP00000000170; ENSRNOG00000000157.
    GeneIDi361321.
    KEGGirno:361321.
    UCSCiRGD:1306306. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC087139 mRNA. Translation: AAH87139.1 .
    BC110056 mRNA. Translation: AAI10057.1 .
    RefSeqi NP_001033584.1. NM_001038495.1.
    UniGenei Rn.6224.

    3D structure databases

    ProteinModelPortali Q2TBJ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 262598. 1 interaction.
    STRINGi 10116.ENSRNOP00000000170.

    Proteomic databases

    PaxDbi Q2TBJ5.
    PRIDEi Q2TBJ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000000170 ; ENSRNOP00000000170 ; ENSRNOG00000000157 .
    GeneIDi 361321.
    KEGGi rno:361321.
    UCSCi RGD:1306306. rat.

    Organism-specific databases

    CTDi 9140.
    RGDi 1306306. Atg12.

    Phylogenomic databases

    eggNOGi NOG236266.
    GeneTreei ENSGT00390000016654.
    HOGENOMi HOG000234863.
    HOVERGENi HBG080875.
    InParanoidi Q2TBJ5.
    KOi K08336.
    OMAi PWRKARP.
    OrthoDBi EOG7XPZ7X.
    PhylomeDBi Q2TBJ5.
    TreeFami TF325131.

    Enzyme and pathway databases

    Reactomei REACT_198997. Negative regulators of RIG-I/MDA5 signaling.

    Miscellaneous databases

    NextBioi 675928.
    PROi Q2TBJ5.

    Gene expression databases

    Genevestigatori Q2TBJ5.

    Family and domain databases

    InterProi IPR007242. Atg12.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR13385. PTHR13385. 1 hit.
    Pfami PF04110. APG12. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney and Placenta.
    2. Cited for: INDUCTION.
    3. "FoxO transcription factors promote autophagy in cardiomyocytes."
      Sengupta A., Molkentin J.D., Yutzey K.E.
      J. Biol. Chem. 284:28319-28331(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiATG12_RAT
    AccessioniPrimary (citable) accession number: Q2TBJ5
    Secondary accession number(s): Q5M9F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3