Q2TBG8 (UCHL3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L3
Short name=UCH-L3
EC=3.4.19.12

Alternative name(s):
Ubiquitin thioesterase L3

Gene names

Name:

UCHL3

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Enzyme regulation	Inhibited by monoubiquitin and diubiquitin By similarity.
Subunit structure	Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrion Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Ubiquitin carboxyl-terminal hydrolase isozyme L3		PRO_0000239741
Regions
	Region	8 – 13	6	Interaction with ubiquitin By similarity
	Region	152 – 159	8	Interaction with ubiquitin By similarity
	Region	219 – 224	6	Interaction with ubiquitin By similarity
Sites
	Active site	95	1	Nucleophile By similarity
	Active site	169	1	Proton donor By similarity
	Site	184	1	Important for enzyme activity By similarity
Amino acid modifications
	Modified residue	130	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TBG8 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 01D145E4352627BB	FASTA	230	26,182
	10 20 30 40 50 60 MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE 70 80 90 100 110 120 KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK 130 140 150 160 170 180 KFLEESASMS PEERARYLEN YDAIRVTHET SAHEGQTEAP NIDEKVDLHF IALVHVDGHL 190 200 210 220 230 YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC110247 mRNA. Translation: AAI10248.1.
IPI	IPI00705749.
RefSeq	NP_001035631.1. NM_001040541.2.
UniGene	Bt.27926.
3D structure databases
ProteinModelPortal	Q2TBG8.
SMR	Q2TBG8. Positions 2-230.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000035448.
Proteomic databases
PRIDE	Q2TBG8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000035577; ENSBTAP00000035448; ENSBTAG00000008024.
GeneID	520170.
KEGG	bta:520170.
Organism-specific databases
CTD	7347.
Phylogenomic databases
eggNOG	NOG327708.
GeneTree	ENSGT00510000046640.
HOGENOM	HOG000182400.
HOVERGEN	HBG075483.
InParanoid	Q2TBG8.
KO	K05609.
OMA	QTEAPNI.
OrthoDB	EOG4HX51V.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20873040.

Entry information

Entry name

UCHL3_BOVIN

Accession

Primary (citable) accession number: Q2TBG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program