Q2TBE6 (P4K2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase type 2-alpha
EC=2.7.1.67

Alternative name(s):
Phosphatidylinositol 4-kinase type II-alpha

Gene names

Name:

Pi4k2a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Cytoplasm By similarity. Membrane By similarity. Membrane raft By similarity. Note: Found in subdomains of the plasma membrane termed non-caveolar membrane rafts By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	membrane raft Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Phosphatidylinositol 4-kinase type 2-alpha		PRO_0000285159
Regions
	Domain	133 – 438	306	PI3K/PI4K
	Compositional bias	39 – 42	4	Poly-Ala
Amino acid modifications
	Modified residue	5	1	Phosphoserine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	44	1	Phosphoserine By similarity
	Modified residue	47	1	Phosphoserine Ref.2 Ref.3 Ref.4
	Modified residue	51	1	Phosphoserine Ref.2 Ref.4
	Modified residue	462	1	Phosphoserine Ref.2
	Modified residue	464	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TBE6 [UniParc]. Last modified May 16, 2006. Version 1. Checksum: 1D7429AA7BF2918B	FASTA	479	54,258
	10 20 30 40 50 60 MDETSPLVSP ERAQPPEYTF PSGSGAHFPQ VPGGAVRVAA AAGSGPSPPC SPGHDRERQP 70 80 90 100 110 120 LLDRARGAAA QGQTHTVAVQ AQALAAQAAV AAHAVQTHRE RNDFPEDPEF EVVVRQAEVA 130 140 150 160 170 180 IECSIYPERI YQGSSGSYFV KDSQGRIVAV FKPKNEEPYG HLNPKWTKWL QKLCCPCCFG 190 200 210 220 230 240 RDCLVLNQGY LSEAGASLVD QKLELNIVPR TKVVYLASET FNYSAIDRVK SRGKRLALEK 250 260 270 280 290 300 VPKVGQRFNR IGLPPKVGSF QLFVEGYKDA DYWLRRFEAE PLPENTNRQL LLQFERLVVL 310 320 330 340 350 360 DYIIRNTDRG NDNWLIKYDC PMDNSSCRDT DWVMVREPVI KVAAIDNGLA FPLKHPDSWR 370 380 390 400 410 420 AYPFYWAWLP QAKVPFSQEI KDLILPKISD PNFIKDLEED LYELFKRDPG FDRGQFHKQI 430 440 450 460 470 AVMRGQILNL TQALKDNKSP LHLVQMPPVI VETARSHQRS ASESYTQSFQ SRKPFFSWW

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-51 AND SER-462, MASS SPECTROMETRY. Tissue: Liver.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47, MASS SPECTROMETRY. Tissue: Melanoma.
[4]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47 AND SER-51, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC110363 mRNA. Translation: AAI10364.1.
IPI	IPI00121277.
RefSeq	NP_663476.1. NM_145501.2.
UniGene	Mm.117037.
3D structure databases
ProteinModelPortal	Q2TBE6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2TBE6.
PTM databases
PhosphoSite	Q2TBE6.
Proteomic databases
PRIDE	Q2TBE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000066778; ENSMUSP00000069284; ENSMUSG00000025178.
GeneID	84095.
KEGG	mmu:84095.
UCSC	uc008hnf.1. mouse.
Organism-specific databases
CTD	55361.
MGI	MGI:1934031. Pi4k2a.
Phylogenomic databases
eggNOG	roNOG09606.
GeneTree	ENSGT00390000010434.
HOGENOM	HBG714361.
HOVERGEN	HBG059542.
InParanoid	Q2TBE6.
OMA	QRFHRIG.
OrthoDB	EOG4VT5X4.
PhylomeDB	Q2TBE6.
Gene expression databases
ArrayExpress	Q2TBE6.
Bgee	Q2TBE6.
CleanEx	MM_PI4K2A.
Genevestigator	Q2TBE6.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. [Graphical view]
KO	K13711.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	350886.
SOURCE	Search...

Entry information

Entry name

P4K2A_MOUSE

Accession

Primary (citable) accession number: Q2TBE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 16, 2006
Last modified:	November 16, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program