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Protein

Dynamin-like 120 kDa protein, mitochondrial

Gene

Opa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:16778770). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Plays a role in mitochondrial genome maintenance (By similarity).By similarity1 Publication
Dynamin-like 120 kDa protein, form S1: Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.By similarity
Isoforms that contain the alternative exon 4b (present in isoform 2 and isoform 3, but not in isoform 1) are required for mitochondrial genome maintenance, possibly by anchoring the mitochondrial nucleoids to the inner mitochondrial membrane.By similarity

Catalytic activityi

GTP + H2O = GDP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi295 – 302GTPSequence analysis8
Nucleotide bindingi398 – 402GTPSequence analysis5
Nucleotide bindingi467 – 470GTPSequence analysis4

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • apoptotic process Source: UniProtKB-KW
  • calcium import into the mitochondrion Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to L-glutamate Source: RGD
  • cellular senescence Source: ParkinsonsUK-UCL
  • cochlea development Source: RGD
  • dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
  • GTP metabolic process Source: UniProtKB
  • inner mitochondrial membrane organization Source: ParkinsonsUK-UCL
  • intracellular distribution of mitochondria Source: RGD
  • membrane tubulation Source: UniProtKB
  • mitochondrial fission Source: GO_Central
  • mitochondrial fusion Source: ParkinsonsUK-UCL
  • mitochondrial genome maintenance Source: UniProtKB
  • mitochondrion morphogenesis Source: RGD
  • mitochondrion organization Source: RGD
  • negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  • negative regulation of release of cytochrome c from mitochondria Source: ParkinsonsUK-UCL
  • positive regulation of cellular response to insulin stimulus Source: RGD
  • positive regulation of dendrite development Source: RGD
  • positive regulation of dendritic spine morphogenesis Source: RGD
  • positive regulation of glucose import in response to insulin stimulus Source: RGD
  • positive regulation of mitochondrial fusion Source: RGD
  • positive regulation of neuron maturation Source: RGD
  • protein complex oligomerization Source: RGD
  • response to curcumin Source: RGD
  • response to electrical stimulus Source: RGD
  • response to muscle activity Source: RGD
  • response to nutrient levels Source: RGD
  • retina development in camera-type eye Source: RGD
  • visual perception Source: ParkinsonsUK-UCL

Keywordsi

Molecular functionHydrolase
Biological processApoptosis, Sensory transduction
LigandGTP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-169911 Regulation of Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-like 120 kDa protein, mitochondrial (EC:3.6.5.5)
Alternative name(s):
Optic atrophy protein 1 homolog
Cleaved into the following chain:
Gene namesi
Name:Opa1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi708423 Opa1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini88 – 96Mitochondrial matrix1 Publication9
Transmembranei97 – 113HelicalSequence analysisAdd BLAST17
Topological domaini114 – 960Mitochondrial intermembrane1 PublicationAdd BLAST847

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 87Mitochondrion1 PublicationAdd BLAST87
ChainiPRO_000025799488 – 960Dynamin-like 120 kDa protein, mitochondrialAdd BLAST873
ChainiPRO_0000257995195 – 960Dynamin-like 120 kDa protein, form S11 Publication1 PublicationAdd BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei228N6-acetyllysineBy similarity1

Post-translational modificationi

PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions.By similarity
Cleavage at position S2 is mediated by YME1L. Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ). This motif is present in isoform 2 and isoform 3, but is absent in the displayed isoform 1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei194 – 195Cleavage at site S11 Publication2

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ2TA68

PTM databases

iPTMnetiQ2TA68
PhosphoSitePlusiQ2TA68
SwissPalmiQ2TA68

Miscellaneous databases

PMAP-CutDBQ2TA68

Expressioni

Tissue specificityi

Expressed in brain as well as retinal ganglion, starbust amacrine and horizontal cells of the retina. Absent from nerve fibers and photoreceptor cells of the retina.1 Publication

Gene expression databases

BgeeiENSRNOG00000001717
GenevisibleiQ2TA68 RN

Interactioni

Subunit structurei

Oligomeric complex consisting of membrane-bound and soluble forms of OPA1. Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms. Binds PARL (By similarity). Interacts with PRELID1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ2TA68, 2 interactors
MINTiQ2TA68

Structurei

3D structure databases

ProteinModelPortaliQ2TA68
SMRiQ2TA68
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini285 – 561Dynamin-type GPROSITE-ProRule annotationAdd BLAST277

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni295 – 302G1 motifPROSITE-ProRule annotation8
Regioni321 – 324G2 motifPROSITE-ProRule annotation4
Regioni398 – 401G3 motifPROSITE-ProRule annotation4
Regioni467 – 470G4 motifPROSITE-ProRule annotation4
Regioni501 – 504G5 motifPROSITE-ProRule annotation4

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili210 – 254Sequence analysisAdd BLAST45
Coiled coili895 – 960Sequence analysisAdd BLAST66

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074851
HOVERGENiHBG019108
InParanoidiQ2TA68
KOiK17079
OMAiYEDWKDG
OrthoDBiEOG091G01NV

Family and domain databases

CDDicd08771 DLP_1, 1 hit
InterProiView protein in InterPro
IPR001401 Dynamin_GTPase
IPR022812 Dynamin_SF
IPR030381 G_DYNAMIN_dom
IPR033047 Opa1
IPR027417 P-loop_NTPase
PANTHERiPTHR11566 PTHR11566, 1 hit
PTHR11566:SF67 PTHR11566:SF67, 1 hit
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PRINTSiPR00195 DYNAMIN
SMARTiView protein in SMART
SM00053 DYNc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51718 G_DYNAMIN_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms may exist.Curated
Isoform 11 Publication (identifier: Q2TA68-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRAGRAALA CEVCQSLVKH SSGVQRNVPL QKLHLVSRSI YRSHHPALKL
60 70 80 90 100
QRPQLRTSFQ QFSSLTNLSL HKLKLSPTKY GYQPRRNFWP ARLAARLLKL
110 120 130 140 150
RYIILGSAVG GGYTAKKTFD EWKDMIPDLS DYKWIVPDFI WEIDEYIDLE
160 170 180 190 200
KIRKALPSSE DLANFAPDLD KIAESLSLLK DFFTAGTPGE TAFRATDHGS
210 220 230 240 250
ESDKHYRKVS DKEKIDQLQE ELLHTQLKYQ RILERLEKEN KELRKLVLQK
260 270 280 290 300
DDKGIHHRKL KKSLIDMYSE VLDVLSDYDA SYNTQDHLPR VVVVGDQSAG
310 320 330 340 350
KTSVLEMIAQ ARIFPRGSGE MMTRSPVKVT LSEGPHHVAL FKDSSREFDL
360 370 380 390 400
TKEEDLAALR HEIELRMRKN VKEGCTVSPE TISLNVKGPG LQRMVLVDLP
410 420 430 440 450
GVINTVTSGM APDTKETIFS ISKAYMQNPN AIILCIQDGS VDAERSIVTD
460 470 480 490 500
LVSQMDPHGR RTIFVLTKVD LAEKNVASPS RIQQIIEGKL FPMKALGYFA
510 520 530 540 550
VVTGKGNSSE SIEAIREYEE EFFQNSKLLK TSMLKAHQVT TRNLSLAVSD
560 570 580 590 600
CFWKMVRESV EQQADSFKAT RFNLETEWKN NYPRLRELDR NELFEKAKNE
610 620 630 640 650
ILDEVISLSQ VTPKHWEEIL QQSLWERVST HVIENIYLPA AQTMNSGTFN
660 670 680 690 700
TTVDIKLKQW TDKQLPNKAV EVAWETLQDE FSRFMTEPKG KEHDDIFDKL
710 720 730 740 750
KEAVKEESIK RHKWNDFAED SLRVIQHNAL EDRSISDKQQ WDAAIYFMEE
760 770 780 790 800
ALQGRLKDTE NAIENMIGPD WKKRWIYWKN RTQEQCVHNE TKNELEKMLK
810 820 830 840 850
VNDEHPAYLA SDEITTVRKN LESRGVEVDP SLIKDTWHQV YRRHFLKTAL
860 870 880 890 900
NHCNLCRRGF YYYQRHFIDS ELECNDVVLF WRIQRMLAIT ANTLRQQLTN
910 920 930 940 950
TEVRRLEKNV KEVLEDFAED GEKKVKLLTG KRVQLAEDLK KVREIQEKLD
960
AFIEALHQEK
Length:960
Mass (Da):111,307
Last modified:January 24, 2006 - v1
Checksum:i0C4BE54FCD7A53B3
GO
Isoform 21 Publication (identifier: Q2TA68-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-158: S → PKLVSEVIEASEPLLLLS
     209-209: V → GLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRKV

Show »
Length:1,014
Mass (Da):117,393
Checksum:i4EA93B590FDF9C77
GO
Isoform 31 Publication (identifier: Q2TA68-3) [UniParc]FASTAAdd to basket
Also known as: 71 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     209-209: V → GLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRKV

Show »
Length:997
Mass (Da):115,561
Checksum:iC56B78234A2E5E80
GO

Sequence cautioni

The sequence AAB51724 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187T → S in AAR04100 (PubMed:15505078).Curated1
Sequence conflicti316R → P in AAS79791 (PubMed:16249510).Curated1
Sequence conflicti687E → G in AAT92526 (Ref. 6) Curated1
Sequence conflicti692E → G in AAT92526 (Ref. 6) Curated1
Sequence conflicti730L → P in AAT92526 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052186158S → PKLVSEVIEASEPLLLLS in isoform 2. 1 Publication1
Alternative sequenceiVSP_052187209V → GLLGELILLQQQIQEHEEEA RRAAGQYSTSYAQQKRKV in isoform 2 and isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY333988 mRNA Translation: AAR04100.1
AY660011 mRNA Translation: AAV97815.1
AY660012 mRNA Translation: AAV97816.1
AY510274 mRNA Translation: AAS79791.1
BC111071 mRNA Translation: AAI11072.1
U93197 mRNA Translation: AAB51724.1 Different initiation.
AY683458 mRNA Translation: AAT92526.1
RefSeqiNP_598269.3, NM_133585.3 [Q2TA68-1]
XP_006248560.1, XM_006248498.1 [Q2TA68-3]
UniGeneiRn.225901
Rn.9783

Genome annotation databases

EnsembliENSRNOT00000002338; ENSRNOP00000002338; ENSRNOG00000001717 [Q2TA68-1]
ENSRNOT00000002343; ENSRNOP00000002343; ENSRNOG00000001717 [Q2TA68-1]
GeneIDi171116
KEGGirno:171116
UCSCiRGD:708423 rat [Q2TA68-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOPA1_RAT
AccessioniPrimary (citable) accession number: Q2TA68
Secondary accession number(s): O08681
, Q5MPP1, Q5MPP2, Q5QJE9, Q6B435, Q6R611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health