Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dynamin-like 120 kDa protein, mitochondrial

Gene

Opa1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. May also play a role in mitochondrial genome maintenance.By similarity1 Publication
Dynamin-like 120 kDa protein, form S1: Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.By similarity

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi295 – 3028GTPSequence analysis
Nucleotide bindingi398 – 4025GTPSequence analysis
Nucleotide bindingi467 – 4704GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • apoptotic process Source: UniProtKB-KW
  • cellular senescence Source: ParkinsonsUK-UCL
  • inner mitochondrial membrane organization Source: ParkinsonsUK-UCL
  • mitochondrial fusion Source: ParkinsonsUK-UCL
  • mitochondrial genome maintenance Source: UniProtKB
  • mitochondrion organization Source: RGD
  • negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  • negative regulation of release of cytochrome c from mitochondria Source: ParkinsonsUK-UCL
  • positive regulation of dendrite development Source: RGD
  • positive regulation of mitochondrial fusion Source: RGD
  • positive regulation of neuron maturation Source: RGD
  • visual perception Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Apoptosis, Sensory transduction

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-like 120 kDa protein, mitochondrial (EC:3.6.5.5)
Alternative name(s):
Optic atrophy protein 1 homolog
Cleaved into the following chain:
Gene namesi
Name:Opa1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi708423. Opa1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini88 – 969Mitochondrial matrix1 Publication
Transmembranei97 – 11317HelicalSequence analysisAdd
BLAST
Topological domaini114 – 960847Mitochondrial intermembrane1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8787Mitochondrion1 PublicationAdd
BLAST
Chaini88 – 960873Dynamin-like 120 kDa protein, mitochondrialPRO_0000257994Add
BLAST
Chaini195 – 960766Dynamin-like 120 kDa protein, form S11 PublicationPRO_0000257995Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei228 – 2281N6-acetyllysineBy similarity

Post-translational modificationi

PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ2TA68.

PTM databases

iPTMnetiQ2TA68.
PhosphoSiteiQ2TA68.
SwissPalmiQ2TA68.

Miscellaneous databases

PMAP-CutDBQ2TA68.

Expressioni

Tissue specificityi

Expressed in brain as well as retinal ganglion, starbust amacrine and horizontal cells of the retina. Absent from nerve fibers and photoreceptor cells of the retina.1 Publication

Gene expression databases

GenevisibleiQ2TA68. RN.

Interactioni

Subunit structurei

Oligomeric complex consisting of membrane-bound and soluble forms of OPA1. Interacts with CHCHD3 and IMMT; these interactions occur preferentially with soluble OPA1 forms. Binds PARL. Interacts with PRELID1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ2TA68. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ2TA68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini285 – 561277Dynamin-type GAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili210 – 25445Sequence analysisAdd
BLAST
Coiled coili895 – 96066Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074851.
HOVERGENiHBG019108.
InParanoidiQ2TA68.
KOiK17079.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001401. Dynamin_GTPase.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR033047. Opa1.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PTHR11566:SF67. PTHR11566:SF67. 1 hit.
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms may exist.Curated

Isoform 11 Publication (identifier: Q2TA68-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRAGRAALA CEVCQSLVKH SSGVQRNVPL QKLHLVSRSI YRSHHPALKL
60 70 80 90 100
QRPQLRTSFQ QFSSLTNLSL HKLKLSPTKY GYQPRRNFWP ARLAARLLKL
110 120 130 140 150
RYIILGSAVG GGYTAKKTFD EWKDMIPDLS DYKWIVPDFI WEIDEYIDLE
160 170 180 190 200
KIRKALPSSE DLANFAPDLD KIAESLSLLK DFFTAGTPGE TAFRATDHGS
210 220 230 240 250
ESDKHYRKVS DKEKIDQLQE ELLHTQLKYQ RILERLEKEN KELRKLVLQK
260 270 280 290 300
DDKGIHHRKL KKSLIDMYSE VLDVLSDYDA SYNTQDHLPR VVVVGDQSAG
310 320 330 340 350
KTSVLEMIAQ ARIFPRGSGE MMTRSPVKVT LSEGPHHVAL FKDSSREFDL
360 370 380 390 400
TKEEDLAALR HEIELRMRKN VKEGCTVSPE TISLNVKGPG LQRMVLVDLP
410 420 430 440 450
GVINTVTSGM APDTKETIFS ISKAYMQNPN AIILCIQDGS VDAERSIVTD
460 470 480 490 500
LVSQMDPHGR RTIFVLTKVD LAEKNVASPS RIQQIIEGKL FPMKALGYFA
510 520 530 540 550
VVTGKGNSSE SIEAIREYEE EFFQNSKLLK TSMLKAHQVT TRNLSLAVSD
560 570 580 590 600
CFWKMVRESV EQQADSFKAT RFNLETEWKN NYPRLRELDR NELFEKAKNE
610 620 630 640 650
ILDEVISLSQ VTPKHWEEIL QQSLWERVST HVIENIYLPA AQTMNSGTFN
660 670 680 690 700
TTVDIKLKQW TDKQLPNKAV EVAWETLQDE FSRFMTEPKG KEHDDIFDKL
710 720 730 740 750
KEAVKEESIK RHKWNDFAED SLRVIQHNAL EDRSISDKQQ WDAAIYFMEE
760 770 780 790 800
ALQGRLKDTE NAIENMIGPD WKKRWIYWKN RTQEQCVHNE TKNELEKMLK
810 820 830 840 850
VNDEHPAYLA SDEITTVRKN LESRGVEVDP SLIKDTWHQV YRRHFLKTAL
860 870 880 890 900
NHCNLCRRGF YYYQRHFIDS ELECNDVVLF WRIQRMLAIT ANTLRQQLTN
910 920 930 940 950
TEVRRLEKNV KEVLEDFAED GEKKVKLLTG KRVQLAEDLK KVREIQEKLD
960
AFIEALHQEK
Length:960
Mass (Da):111,307
Last modified:January 24, 2006 - v1
Checksum:i0C4BE54FCD7A53B3
GO
Isoform 21 Publication (identifier: Q2TA68-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-158: S → PKLVSEVIEASEPLLLLS
     209-209: V → GLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRKV

Show »
Length:1,014
Mass (Da):117,393
Checksum:i4EA93B590FDF9C77
GO
Isoform 31 Publication (identifier: Q2TA68-3) [UniParc]FASTAAdd to basket

Also known as: 71 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     209-209: V → GLLGELILLQQQIQEHEEEARRAAGQYSTSYAQQKRKV

Show »
Length:997
Mass (Da):115,561
Checksum:iC56B78234A2E5E80
GO

Sequence cautioni

The sequence AAB51724.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871T → S in AAR04100 (PubMed:15505078).Curated
Sequence conflicti316 – 3161R → P in AAS79791 (PubMed:16249510).Curated
Sequence conflicti687 – 6871E → G in AAT92526 (Ref. 6) Curated
Sequence conflicti692 – 6921E → G in AAT92526 (Ref. 6) Curated
Sequence conflicti730 – 7301L → P in AAT92526 (Ref. 6) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei158 – 1581S → PKLVSEVIEASEPLLLLS in isoform 2. 1 PublicationVSP_052186
Alternative sequencei209 – 2091V → GLLGELILLQQQIQEHEEEA RRAAGQYSTSYAQQKRKV in isoform 2 and isoform 3. 1 PublicationVSP_052187

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY333988 mRNA. Translation: AAR04100.1.
AY660011 mRNA. Translation: AAV97815.1.
AY660012 mRNA. Translation: AAV97816.1.
AY510274 mRNA. Translation: AAS79791.1.
BC111071 mRNA. Translation: AAI11072.1.
U93197 mRNA. Translation: AAB51724.1. Different initiation.
AY683458 mRNA. Translation: AAT92526.1.
RefSeqiNP_598269.3. NM_133585.3. [Q2TA68-1]
XP_006248560.1. XM_006248498.1. [Q2TA68-3]
UniGeneiRn.225901.
Rn.9783.

Genome annotation databases

EnsembliENSRNOT00000002338; ENSRNOP00000002338; ENSRNOG00000001717. [Q2TA68-1]
ENSRNOT00000002343; ENSRNOP00000002343; ENSRNOG00000001717. [Q2TA68-1]
GeneIDi171116.
KEGGirno:171116.
UCSCiRGD:708423. rat. [Q2TA68-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY333988 mRNA. Translation: AAR04100.1.
AY660011 mRNA. Translation: AAV97815.1.
AY660012 mRNA. Translation: AAV97816.1.
AY510274 mRNA. Translation: AAS79791.1.
BC111071 mRNA. Translation: AAI11072.1.
U93197 mRNA. Translation: AAB51724.1. Different initiation.
AY683458 mRNA. Translation: AAT92526.1.
RefSeqiNP_598269.3. NM_133585.3. [Q2TA68-1]
XP_006248560.1. XM_006248498.1. [Q2TA68-3]
UniGeneiRn.225901.
Rn.9783.

3D structure databases

ProteinModelPortaliQ2TA68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ2TA68. 2 interactions.

PTM databases

iPTMnetiQ2TA68.
PhosphoSiteiQ2TA68.
SwissPalmiQ2TA68.

Proteomic databases

PRIDEiQ2TA68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002338; ENSRNOP00000002338; ENSRNOG00000001717. [Q2TA68-1]
ENSRNOT00000002343; ENSRNOP00000002343; ENSRNOG00000001717. [Q2TA68-1]
GeneIDi171116.
KEGGirno:171116.
UCSCiRGD:708423. rat. [Q2TA68-1]

Organism-specific databases

CTDi4976.
RGDi708423. Opa1.

Phylogenomic databases

GeneTreeiENSGT00550000074851.
HOVERGENiHBG019108.
InParanoidiQ2TA68.
KOiK17079.

Miscellaneous databases

NextBioi621844.
PMAP-CutDBQ2TA68.
PROiQ2TA68.

Gene expression databases

GenevisibleiQ2TA68. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001401. Dynamin_GTPase.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR033047. Opa1.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 1 hit.
PTHR11566:SF67. PTHR11566:SF67. 1 hit.
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "OPA1, the disease gene for autosomal dominant optic atrophy, is specifically expressed in ganglion cells and intrinsic neurons of the retina."
    Pesch U.E.A., Fries J.E., Bette S., Kalbacher H., Wissinger B., Alexander C., Kohler K.
    Invest. Ophthalmol. Vis. Sci. 45:4217-4225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY.
    Tissue: Retinal ganglion1 Publication.
  2. "Expression of the Opa1 mitochondrial protein in retinal ganglion cells: its downregulation causes aggregation of the mitochondrial network."
    Kamei S., Chen-Kuo-Chang M., Cazevieille C., Lenaers G., Olichon A., Belenguer P., Roussignol G., Renard N., Eybalin M., Michelin A., Delettre C., Brabet P., Hamel C.P.
    Invest. Ophthalmol. Vis. Sci. 46:4288-4294(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: BrainImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: LiverImported.
  4. Lubec G., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 302-312; 416-423; 446-460; 801-818 AND 825-834, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  5. "A novel gene in the rat genome."
    Yuan X.J., Salgar S.K., Kunz H.W., Gill T.J. III
    Rat Genome 2:150-153(1996)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 556-960.
    Strain: R21Imported.
    Tissue: SpleenImported.
  6. "Differential gene expression in gut during rat fetal period."
    Fabregas P.J., Nebot-Cegarra J., Capella G., Peinado M.A.
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 686-788.
    Strain: OFAImported.
    Tissue: Intestine.
  7. "Regulation of mitochondrial morphology through proteolytic cleavage of OPA1."
    Ishihara N., Fujita Y., Oka T., Mihara K.
    EMBO J. 25:2966-2977(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiOPA1_RAT
AccessioniPrimary (citable) accession number: Q2TA68
Secondary accession number(s): O08681
, Q5MPP1, Q5MPP2, Q5QJE9, Q6B435, Q6R611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.