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Protein

Thymidylate synthase

Gene

TYMS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981By similarity

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TYMS
Synonyms:TS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Mitochondrion By similarity. Mitochondrion matrix By similarity. Mitochondrion inner membrane By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. mitochondrial inner membrane Source: UniProtKB
  3. mitochondrial matrix Source: UniProtKB
  4. mitochondrion Source: UniProtKB
  5. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 354353Thymidylate synthasePRO_0000317541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei117 – 1171PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ2TA32.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009214.

Structurei

3D structure databases

ProteinModelPortaliQ2TA32.
SMRiQ2TA32. Positions 31-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
HOVERGENiHBG001934.
InParanoidiQ2TA32.
KOiK00560.

Family and domain databases

Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 2 hits.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 2 hits.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TA32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAAGSEPSR PPSPPGVQEQ SAEPRPPPPP HGELQYLGQI EHILRCGFRR
60 70 80 90 100
DDRTGTGTLS VFGMQARYNL RDEFPLLTTK RVFWKGVLEE LLWFIKGSTN
110 120 130 140 150
AKELSSKGVK IWDANGSRDF LDGLGFSDRA EGDLGPVYGF QWRHFGAEYK
160 170 180 190 200
DMDSEYSGQG VDQLQKVIDT IKTNPNDRRI ILCAWNPKDL PLMALPPCHA
210 220 230 240 250
LCQFYVVNGE LSCQLYQRSG DMGLGVPFNI ASYALLTYMI AHITDLKPGD
260 270 280 290 300
FVHTLGDAHI YLNHIEPLKT QALMELRGQS SRSLDGDGQA GTSRWAPVAT
310 320 330 340 350
DTERDRCCEL QREPRPFPKL KILRKVETID DFQAEDFQIE GYNPNPTIKM

EMAV
Length:354
Mass (Da):39,785
Last modified:January 24, 2006 - v1
Checksum:i2350409479AF4C40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111139 mRNA. Translation: AAI11140.1.
AH009263 Genomic DNA. Translation: AAF66224.1.
RefSeqiNP_001032905.1. NM_001037816.1.
UniGeneiBt.7738.

Genome annotation databases

GeneIDi507631.
KEGGibta:507631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111139 mRNA. Translation: AAI11140.1.
AH009263 Genomic DNA. Translation: AAF66224.1.
RefSeqiNP_001032905.1. NM_001037816.1.
UniGeneiBt.7738.

3D structure databases

ProteinModelPortaliQ2TA32.
SMRiQ2TA32. Positions 31-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009214.

Proteomic databases

PRIDEiQ2TA32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi507631.
KEGGibta:507631.

Organism-specific databases

CTDi7298.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
HOVERGENiHBG001934.
InParanoidiQ2TA32.
KOiK00560.

Enzyme and pathway databases

UniPathwayiUPA00575.

Miscellaneous databases

NextBioi20868149.

Family and domain databases

Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 2 hits.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 2 hits.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  2. "Estimate of nucleotide diversity in dogs with a pool-and-sequence method."
    Brouillette J.A., Andrew J.R., Venta P.J.
    Mamm. Genome 11:1079-1086(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-255.

Entry informationi

Entry nameiTYSY_BOVIN
AccessioniPrimary (citable) accession number: Q2TA32
Secondary accession number(s): Q9N1D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 24, 2006
Last modified: March 4, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.