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Protein

Thymidylate synthase

Gene

TYMS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53dUMPBy similarity1
Active sitei198NucleophileBy similarity1
Binding sitei2215,10-methylenetetrahydrofolateBy similarity1
Binding sitei229dUMPBy similarity1
Binding sitei3535,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi218 – 221dUMPBy similarity4
Nucleotide bindingi259 – 261dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TYMS
Synonyms:TS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243905.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003175412 – 354Thymidylate synthaseAdd BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2TA32.
PRIDEiQ2TA32.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009214.

Chemistry databases

BindingDBiQ2TA32.

Structurei

3D structure databases

ProteinModelPortaliQ2TA32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
HOGENOMiHOG000257899.
HOVERGENiHBG001934.
InParanoidiQ2TA32.
KOiK00560.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 2 hits.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 2 hits.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2TA32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAAGSEPSR PPSPPGVQEQ SAEPRPPPPP HGELQYLGQI EHILRCGFRR
60 70 80 90 100
DDRTGTGTLS VFGMQARYNL RDEFPLLTTK RVFWKGVLEE LLWFIKGSTN
110 120 130 140 150
AKELSSKGVK IWDANGSRDF LDGLGFSDRA EGDLGPVYGF QWRHFGAEYK
160 170 180 190 200
DMDSEYSGQG VDQLQKVIDT IKTNPNDRRI ILCAWNPKDL PLMALPPCHA
210 220 230 240 250
LCQFYVVNGE LSCQLYQRSG DMGLGVPFNI ASYALLTYMI AHITDLKPGD
260 270 280 290 300
FVHTLGDAHI YLNHIEPLKT QALMELRGQS SRSLDGDGQA GTSRWAPVAT
310 320 330 340 350
DTERDRCCEL QREPRPFPKL KILRKVETID DFQAEDFQIE GYNPNPTIKM

EMAV
Length:354
Mass (Da):39,785
Last modified:January 24, 2006 - v1
Checksum:i2350409479AF4C40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111139 mRNA. Translation: AAI11140.1.
AH009263 Genomic DNA. Translation: AAF66224.1.
RefSeqiNP_001032905.1. NM_001037816.1.
UniGeneiBt.7738.

Genome annotation databases

GeneIDi507631.
KEGGibta:507631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111139 mRNA. Translation: AAI11140.1.
AH009263 Genomic DNA. Translation: AAF66224.1.
RefSeqiNP_001032905.1. NM_001037816.1.
UniGeneiBt.7738.

3D structure databases

ProteinModelPortaliQ2TA32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009214.

Chemistry databases

BindingDBiQ2TA32.
ChEMBLiCHEMBL3243905.

Proteomic databases

PaxDbiQ2TA32.
PRIDEiQ2TA32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi507631.
KEGGibta:507631.

Organism-specific databases

CTDi7298.

Phylogenomic databases

eggNOGiKOG0673. Eukaryota.
COG0207. LUCA.
HOGENOMiHOG000257899.
HOVERGENiHBG001934.
InParanoidiQ2TA32.
KOiK00560.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 2 hits.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 2 hits.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_BOVIN
AccessioniPrimary (citable) accession number: Q2TA32
Secondary accession number(s): Q9N1D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.