Q2TA32 (TYSY_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	TYMS
Synonyms:	TS

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Contributes to the de novo mitochondrial thymidylate biosynthesis pathway By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Mitochondrion By similarity. Mitochondrion matrix By similarity. Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion inner membrane Nucleus
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial inner membrane Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial matrix Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 354	353	Thymidylate synthase		PRO_0000317541
Sites
	Active site	198	1	By similarity
Amino acid modifications
	Modified residue	117	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TA32 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 2350409479AF4C40	FASTA	354	39,785
	10 20 30 40 50 60 MPAAGSEPSR PPSPPGVQEQ SAEPRPPPPP HGELQYLGQI EHILRCGFRR DDRTGTGTLS 70 80 90 100 110 120 VFGMQARYNL RDEFPLLTTK RVFWKGVLEE LLWFIKGSTN AKELSSKGVK IWDANGSRDF 130 140 150 160 170 180 LDGLGFSDRA EGDLGPVYGF QWRHFGAEYK DMDSEYSGQG VDQLQKVIDT IKTNPNDRRI 190 200 210 220 230 240 ILCAWNPKDL PLMALPPCHA LCQFYVVNGE LSCQLYQRSG DMGLGVPFNI ASYALLTYMI 250 260 270 280 290 300 AHITDLKPGD FVHTLGDAHI YLNHIEPLKT QALMELRGQS SRSLDGDGQA GTSRWAPVAT 310 320 330 340 350 DTERDRCCEL QREPRPFPKL KILRKVETID DFQAEDFQIE GYNPNPTIKM EMAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[2]	"Estimate of nucleotide diversity in dogs with a pool-and-sequence method." Brouillette J.A., Andrew J.R., Venta P.J. Mamm. Genome 11:1079-1086(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 211-255.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC111139 mRNA. Translation: AAI11140.1. AH009263 Genomic DNA. Translation: AAF66224.1.
IPI	IPI00708073.
RefSeq	NP_001032905.1. NM_001037816.1.
UniGene	Bt.7738.
3D structure databases
HSSP	HSSP built from PDB template 1JUJ based on UniProtKB P04818.
ProteinModelPortal	Q2TA32.
SMR	Q2TA32. Positions 31-354.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000009214.
Proteomic databases
PRIDE	Q2TA32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	507631.
KEGG	bta:507631.
Organism-specific databases
CTD	7298.
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
HOVERGEN	HBG001934.
InParanoid	Q2TA32.
KO	K00560.
OrthoDB	EOG4ZKJMP.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 2 hits.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 2 hits. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 2 hits.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20868149.

Entry information

Entry name

TYSY_BOVIN

Accession

Primary (citable) accession number: Q2TA32
Secondary accession number(s): Q9N1D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program