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Protein

Ubiquitin-conjugating enzyme E2 D1

Gene

UBE2D1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4 (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_274605. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
REACT_280577. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_291730. Separation of Sister Chromatids.
REACT_294563. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_294798. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_304977. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_307894. Regulation of APC/C activators between G1/S and early anaphase.
REACT_309977. Phosphorylation of the APC/C.
REACT_310911. Signaling by BMP.
REACT_326365. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_333795. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_335347. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_338497. APC/C:Cdc20 mediated degradation of Securin.
REACT_345107. IKK complex recruitment mediated by RIP1.
REACT_345506. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
REACT_351362. Senescence-Associated Secretory Phenotype (SASP).
REACT_353435. APC-Cdc20 mediated degradation of Nek2A.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 D1 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein D1
Ubiquitin-protein ligase D1
Gene namesi
Name:UBE2D1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Ubiquitin-conjugating enzyme E2 D1PRO_0000245033Add
BLAST

Interactioni

Subunit structurei

Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with RNF11.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051358.

Structurei

3D structure databases

ProteinModelPortaliQ2TA10.
SMRiQ2TA10. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ2TA10.
KOiK06689.
OMAiSAGPCED.
OrthoDBiEOG7PCJGX.
TreeFamiTF101108.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2TA10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRIQKEL SDLQRDPPAH CSAGPVGDDL FHWQATIMGP PDSAYQGGVF
60 70 80 90 100
FLTVHFPTDY PFKPPKIAFT TKIYHPNINS NGSICLDILR SQWSPALTVS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PDIAQIYKSD KEKYNRHARE WTQKYAM
Length:147
Mass (Da):16,602
Last modified:January 24, 2006 - v1
Checksum:i2E96FD0179EE119D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111175 mRNA. Translation: AAI11176.1.
RefSeqiNP_001033256.1. NM_001038167.1.
UniGeneiBt.52062.

Genome annotation databases

EnsembliENSBTAT00000055368; ENSBTAP00000051358; ENSBTAG00000020796.
GeneIDi535287.
KEGGibta:535287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111175 mRNA. Translation: AAI11176.1.
RefSeqiNP_001033256.1. NM_001038167.1.
UniGeneiBt.52062.

3D structure databases

ProteinModelPortaliQ2TA10.
SMRiQ2TA10. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000055368; ENSBTAP00000051358; ENSBTAG00000020796.
GeneIDi535287.
KEGGibta:535287.

Organism-specific databases

CTDi7321.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ2TA10.
KOiK06689.
OMAiSAGPCED.
OrthoDBiEOG7PCJGX.
TreeFamiTF101108.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiREACT_274605. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
REACT_280577. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_291730. Separation of Sister Chromatids.
REACT_294563. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_294798. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_304977. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_307894. Regulation of APC/C activators between G1/S and early anaphase.
REACT_309977. Phosphorylation of the APC/C.
REACT_310911. Signaling by BMP.
REACT_326365. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_333795. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_335347. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_338497. APC/C:Cdc20 mediated degradation of Securin.
REACT_345107. IKK complex recruitment mediated by RIP1.
REACT_345506. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
REACT_351362. Senescence-Associated Secretory Phenotype (SASP).
REACT_353435. APC-Cdc20 mediated degradation of Nek2A.

Miscellaneous databases

NextBioi20876683.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiUB2D1_BOVIN
AccessioniPrimary (citable) accession number: Q2TA10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 24, 2006
Last modified: July 22, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.