Reviewed, UniProtKB/Swiss-Prot Q2TA03 (UB2J2_BOVIN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 J2
    EC=6.3.2.19

Gene names

Name:

UBE2J2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Ubiquitin-conjugating enzyme E2 J2		PRO_0000245041
Regions
	Topological domain	1 – 226	226	Cytoplasmic Potential
	Transmembrane	227 – 247	21	Anchor for type IV membrane protein Potential
	Topological domain	248 – 259	12	Lumenal Potential
Sites
	Active site	94	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2TA03-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: AFF8D77BD15BF8A4	FASTA	259	28,848
	10 20 30 40 50 60 MSSNSVKRAP TTATQRLKQD YLRIKKDPVP YICAEPLPSN ILEWHYVVRG PEMTPYEGGY 70 80 90 100 110 120 YHGKLIFPRE FPFKPPSIYM ITPNGRFKCN TRLCLSITDF HPDTWNPAWS VSTILTGLLS 130 140 150 160 170 180 FMVEKGPTLG SIETSDFTKR QLAAQSLVFN LKDKVFCELF PEVVEEIKQK QKAQDELSSR 190 200 210 220 230 240 PQALPLPDVV PDGETHHGQH GLPLLNGHAP GAGPHLAGLQ QANRHHGLLG GALANLFVIV 250 GFAAFAYTVK YVLRSIAQE

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC111185 mRNA. Translation: AAI11186.1.
IPI	IPI00701501.
RefSeq	NP_001033132.1.
UniGene	Bt.32465
3D structure databases
SMR	Q2TA03. Positions 11-171.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000021109; ENSBTAP00000021109; ENSBTAG00000015882; Bos taurus. [Genome view]
GeneID	506103.
KEGG	bta:506103.
Organism-specific databases
CTD	506103.
Phylogenomic databases
eggNOG	maNOG07586.
HOVERGEN	Q2TA03.
InParanoid	Q2TA03.
OMA	ETSDYTK.
OrthoDB	EOG9XPT20.
PhylomeDB	Q2TA03.
Enzyme and pathway databases
BRENDA	6.3.2.19. 251.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB2J2_BOVIN

Accession

Primary (citable) accession number: Q2TA03

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	January 24, 2006
Last modified:	January 19, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)