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Protein

Glutamate--cysteine ligase regulatory subunit

Gene

GCLM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase regulatory subunit (GCLM)
  2. Glutathione synthetase (GSS), Glutathione synthetase (GSS)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Glutathione biosynthesis

Enzyme and pathway databases

ReactomeiR-BTA-1614635. Sulfur amino acid metabolism.
R-BTA-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase regulatory subunit
Alternative name(s):
GCS light chain
Gamma-ECS regulatory subunit
Gamma-glutamylcysteine synthetase regulatory subunit
Glutamate--cysteine ligase modifier subunit
Gene namesi
Name:GCLM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002537311 – 274Glutamate--cysteine ligase regulatory subunitAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphoserineBy similarity1
Modified residuei263N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2T9Y6.
PRIDEiQ2T9Y6.

Expressioni

Gene expression databases

BgeeiENSBTAG00000007842.

Interactioni

Subunit structurei

Heterodimer of a catalytic heavy chain and a regulatory light chain.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026855.

Structurei

3D structure databases

ProteinModelPortaliQ2T9Y6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3023. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00510000047658.
HOGENOMiHOG000007111.
HOVERGENiHBG005923.
InParanoidiQ2T9Y6.
KOiK11205.
OMAiEYLQPYW.
OrthoDBiEOG091G0DYU.
TreeFamiTF105986.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR032963. Gclm.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR13295. PTHR13295. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
SUPFAMiSSF51430. SSF51430. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2T9Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT
60 70 80 90 100
LNEWSSQISP DLIREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVGS
110 120 130 140 150
NSSSSTRNAV DMACSVLGVA QLDSVIIASP PVEDGVNLSL EHLQPYWEEL
160 170 180 190 200
QNLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
210 220 230 240 250
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIRAH EWVPLWLLRY
260 270
SVIVKSRGII KSKGYILQAK RKGS
Length:274
Mass (Da):30,533
Last modified:January 24, 2006 - v1
Checksum:iD1E5B90BE45D6008
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111206 mRNA. Translation: AAI11207.1.
RefSeqiNP_001033232.1. NM_001038143.1.
UniGeneiBt.20267.

Genome annotation databases

EnsembliENSBTAT00000026855; ENSBTAP00000026855; ENSBTAG00000007842.
GeneIDi525659.
KEGGibta:525659.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111206 mRNA. Translation: AAI11207.1.
RefSeqiNP_001033232.1. NM_001038143.1.
UniGeneiBt.20267.

3D structure databases

ProteinModelPortaliQ2T9Y6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026855.

Proteomic databases

PaxDbiQ2T9Y6.
PRIDEiQ2T9Y6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026855; ENSBTAP00000026855; ENSBTAG00000007842.
GeneIDi525659.
KEGGibta:525659.

Organism-specific databases

CTDi2730.

Phylogenomic databases

eggNOGiKOG3023. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00510000047658.
HOGENOMiHOG000007111.
HOVERGENiHBG005923.
InParanoidiQ2T9Y6.
KOiK11205.
OMAiEYLQPYW.
OrthoDBiEOG091G0DYU.
TreeFamiTF105986.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
ReactomeiR-BTA-1614635. Sulfur amino acid metabolism.
R-BTA-174403. Glutathione synthesis and recycling.

Gene expression databases

BgeeiENSBTAG00000007842.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR032963. Gclm.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR13295. PTHR13295. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
SUPFAMiSSF51430. SSF51430. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSH0_BOVIN
AccessioniPrimary (citable) accession number: Q2T9Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: January 24, 2006
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.