Q2T9X7 (UB2E3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 E3
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein E3
Ubiquitin-protein ligase E3

Gene names

Name:

UBE2E3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus. Interacts with NEDD4L By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Growth regulation Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Ubiquitin-conjugating enzyme E2 E3		PRO_0000245036
Sites
	Active site	145	1	Glycyl thioester intermediate
Amino acid modifications
	Modified residue	3	1	Phosphoserine By similarity
	Modified residue	12	1	Phosphoserine By similarity
	Modified residue	91	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T9X7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 821CB1382478DC9F	FASTA	207	22,913
	10 20 30 40 50 60 MSSDRQRSDD ESPSTSSGSS DADQRDPAAP EPEEQEERKP SATQQKKNTK LSSKTTAKLS 70 80 90 100 110 120 TSAKRIQKEL AEITLDPPPN CSAGPKGDNI YEWRSTILGP PGSVYEGGVF FLDITFSSDY 130 140 150 160 170 180 PFKPPKVTFR TRIYHCNINS QGVICLDILK DNWSPALTIS KVLLSICSLL TDCNPADPLV 190 200 GSIATQYLTN RAEHDRIARQ WTKRYAT

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC111218 mRNA. Translation: AAI11219.1.
IPI	IPI00693726.
RefSeq	NP_001073251.1. NM_001079783.1.
UniGene	Bt.55273.
3D structure databases
ProteinModelPortal	Q2T9X7.
SMR	Q2T9X7. Positions 60-207.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T9X7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	534349.
KEGG	bta:534349.
Organism-specific databases
CTD	10477.
Phylogenomic databases
eggNOG	maNOG17146.
GeneTree	ENSGT00590000082778.
HOVERGEN	HBG063308.
OrthoDB	EOG48SGV9.
PhylomeDB	Q2T9X7.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K06689.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UB2E3_BOVIN

Accession

Primary (citable) accession number: Q2T9X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	January 24, 2006
Last modified:	November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program