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Q2T9L8 (SYMC_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase, cytoplasmic
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:MARS
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length898 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Subunit structure

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 GST C-terminal domain.

Contains 1 WHEP-TRS domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 898898Methionine--tRNA ligase, cytoplasmic
PRO_0000246786

Regions

Domain74 – 198125GST C-terminal
Domain839 – 89557WHEP-TRS
Motif273 – 28311"HIGH" region
Motif593 – 5975"KMSKS" region

Sites

Binding site5961ATP By similarity

Amino acid modifications

Modified residue7261N6-acetyllysine By similarity
Modified residue8251Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2T9L8 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 174EFB5712AE15CD

FASTA898100,634
        10         20         30         40         50         60 
MRLFVSEGAP GSLPVLAAAG RAQGRAELLI STVGPEECVV PFLTRPKVPV LQLDSGNYLF 

        70         80         90        100        110        120 
STSAICRYFF LLSGWEQDDL TNQWLEWEAT ELQPALSAAL YYLVVQGKKG EDVLGPVRRA 

       130        140        150        160        170        180 
LTHIDHSLSR RSCPFLAGET ESLADIVLWG ALYPLLQDPS YLPEELGALH SWFQTLSSQE 

       190        200        210        220        230        240 
PCQRAAETVL KQQGVLALRP YLQKQPQPSS LEGRLVSNEP EEEELATLSE EEIAVAVAAW 

       250        260        270        280        290        300 
EKGLESLPPL RPQQNPVLPV AGERNVLITS ALPYVNNVPH LGNIIGCVLS ADVFARYSRL 

       310        320        330        340        350        360 
RQWNTLYLCG TDEYGTATET KAMEEGLTPQ EICDKYHVIH ADIYRWFNIS FDFFGRTTTP 

       370        380        390        400        410        420 
QQTKITQDIF QRLLARGFVL QDTVEQLRCE HCARFLADRF VEGVCPFCGY EEARGDQCDK 

       430        440        450        460        470        480 
CGKLINAIEL KKPQCKVCRS CPVVKSSQHL FLDLPKLAAR VEEWLEKTLP GSDWTANARF 

       490        500        510        520        530        540 
IIRSWLRDGL KPRCITRDLK WGTPVPLEGF EDKVFYVWFD ATIGYLSITA NYTDQWEKWW 

       550        560        570        580        590        600 
KNPEQVNLYQ FMAKDNVPFH GIVFPSSALG AEDNYTLVSH LIATEYLNYE DGKFSKSRGV 

       610        620        630        640        650        660 
GVFGDMAQDT GIPADIWRFY LLYNRPEGQD SAFSWTDMLF KNNSELLNNL GNFINRAGMF 

       670        680        690        700        710        720 
VSKFFGGFVP EMVLTSDDQR LLTHITLELQ HYHQLLEKVR IRDALRSILT ISRHGNQYIQ 

       730        740        750        760        770        780 
VNEPWKRIKG GEADRQRAGT VTGLAVNIAA LLSVMLQPYM PTVSATIQAQ LQLPPPACSI 

       790        800        810        820        830        840 
LPTNFLCTLP AGHQIGTVSP LFQKLENDQI ESLKQRFSGG QAKASPKTAA GLTTAGPQQI 

       850        860        870        880        890 
QALTEEVTKQ GNIVRELKAQ KADKNQIAAE VAKLLDLKKQ LALAEGKPLE TSKGKKKK

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC111362 mRNA. Translation: AAI11363.1.
IPIIPI00718025.
RefSeqNP_001033180.1. NM_001038091.1.
UniGeneBt.9306.

3D structure databases

ProteinModelPortalQ2T9L8.
SMRQ2T9L8. Positions 833-890.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2T9L8.

Proteomic databases

PRIDEQ2T9L8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID512531.
KEGGbta:512531.

Organism-specific databases

CTD4141.

Phylogenomic databases

eggNOGmaNOG11063.
GeneTreeENSGT00550000075017.
HOVERGENHBG036191.
InParanoidQ2T9L8.
OrthoDBEOG4Z0B50.
PhylomeDBQ2T9L8.

Family and domain databases

InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR000738. WHEP-TRS.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SMARTSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMSSF47616. GST_C_like. 1 hit.
SSF47060. S15/NS1_bind. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYMC_BOVIN
AccessionPrimary (citable) accession number: Q2T9L8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 24, 2006
Last modified: January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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