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Protein

Methionine--tRNA ligase, cytoplasmic

Gene

MARS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei596ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase, cytoplasmic (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:MARS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002467861 – 898Methionine--tRNA ligase, cytoplasmicAdd BLAST898

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei825PhosphoserineBy similarity1
Modified residuei833PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2T9L8.
PeptideAtlasiQ2T9L8.
PRIDEiQ2T9L8.

Interactioni

Subunit structurei

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024487.

Structurei

3D structure databases

ProteinModelPortaliQ2T9L8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 198GST C-terminalAdd BLAST125
Domaini839 – 895WHEP-TRSAdd BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi273 – 283"HIGH" regionAdd BLAST11
Motifi593 – 597"KMSKS" region5

Sequence similaritiesi

Contains 1 GST C-terminal domain.Curated
Contains 1 WHEP-TRS domain.Curated

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1247. Eukaryota.
COG0143. LUCA.
HOGENOMiHOG000200402.
HOVERGENiHBG036191.
InParanoidiQ2T9L8.
KOiK01874.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.287.10. 1 hit.
1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2T9L8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFVSEGAP GSLPVLAAAG RAQGRAELLI STVGPEECVV PFLTRPKVPV
60 70 80 90 100
LQLDSGNYLF STSAICRYFF LLSGWEQDDL TNQWLEWEAT ELQPALSAAL
110 120 130 140 150
YYLVVQGKKG EDVLGPVRRA LTHIDHSLSR RSCPFLAGET ESLADIVLWG
160 170 180 190 200
ALYPLLQDPS YLPEELGALH SWFQTLSSQE PCQRAAETVL KQQGVLALRP
210 220 230 240 250
YLQKQPQPSS LEGRLVSNEP EEEELATLSE EEIAVAVAAW EKGLESLPPL
260 270 280 290 300
RPQQNPVLPV AGERNVLITS ALPYVNNVPH LGNIIGCVLS ADVFARYSRL
310 320 330 340 350
RQWNTLYLCG TDEYGTATET KAMEEGLTPQ EICDKYHVIH ADIYRWFNIS
360 370 380 390 400
FDFFGRTTTP QQTKITQDIF QRLLARGFVL QDTVEQLRCE HCARFLADRF
410 420 430 440 450
VEGVCPFCGY EEARGDQCDK CGKLINAIEL KKPQCKVCRS CPVVKSSQHL
460 470 480 490 500
FLDLPKLAAR VEEWLEKTLP GSDWTANARF IIRSWLRDGL KPRCITRDLK
510 520 530 540 550
WGTPVPLEGF EDKVFYVWFD ATIGYLSITA NYTDQWEKWW KNPEQVNLYQ
560 570 580 590 600
FMAKDNVPFH GIVFPSSALG AEDNYTLVSH LIATEYLNYE DGKFSKSRGV
610 620 630 640 650
GVFGDMAQDT GIPADIWRFY LLYNRPEGQD SAFSWTDMLF KNNSELLNNL
660 670 680 690 700
GNFINRAGMF VSKFFGGFVP EMVLTSDDQR LLTHITLELQ HYHQLLEKVR
710 720 730 740 750
IRDALRSILT ISRHGNQYIQ VNEPWKRIKG GEADRQRAGT VTGLAVNIAA
760 770 780 790 800
LLSVMLQPYM PTVSATIQAQ LQLPPPACSI LPTNFLCTLP AGHQIGTVSP
810 820 830 840 850
LFQKLENDQI ESLKQRFSGG QAKASPKTAA GLTTAGPQQI QALTEEVTKQ
860 870 880 890
GNIVRELKAQ KADKNQIAAE VAKLLDLKKQ LALAEGKPLE TSKGKKKK
Length:898
Mass (Da):100,634
Last modified:January 24, 2006 - v1
Checksum:i174EFB5712AE15CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111362 mRNA. Translation: AAI11363.1.
RefSeqiNP_001033180.1. NM_001038091.2.
UniGeneiBt.9306.

Genome annotation databases

GeneIDi512531.
KEGGibta:512531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111362 mRNA. Translation: AAI11363.1.
RefSeqiNP_001033180.1. NM_001038091.2.
UniGeneiBt.9306.

3D structure databases

ProteinModelPortaliQ2T9L8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024487.

Proteomic databases

PaxDbiQ2T9L8.
PeptideAtlasiQ2T9L8.
PRIDEiQ2T9L8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi512531.
KEGGibta:512531.

Organism-specific databases

CTDi4141.

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
KOG1247. Eukaryota.
COG0143. LUCA.
HOGENOMiHOG000200402.
HOVERGENiHBG036191.
InParanoidiQ2T9L8.
KOiK01874.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.287.10. 1 hit.
1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
2.20.28.20. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR029038. MetRS_Zn.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009068. S15_NS1_RNA-bd.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYMC_BOVIN
AccessioniPrimary (citable) accession number: Q2T9L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.