Q2T8Y6 (CHEB2_BURTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 2
EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	BTH_II0161

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.
Sequence caution	The sequence ABC35113.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000264268
Regions
	Domain	3 – 120	118	Response regulatory
	Domain	164 – 354	191	CheB-type methylesterase
Sites
	Active site	176	1	By similarity
	Active site	202	1	By similarity
	Active site	298	1	By similarity
Amino acid modifications
	Modified residue	54	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T8Y6 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: C113B279BEF61B90	FASTA	354	37,092
	10 20 30 40 50 60 MIRVVVVDDS MSMRTLLERI INGCDGMTCV GAAEDASAAR EMIRALDPDV VTLDVEMPGM 70 80 90 100 110 120 DGLEFLRRMM LLKPTPTIMV SGRTTSGSDA ALRALELGAV DVIAKPLLTR PADLADYARD 130 140 150 160 170 180 IAELIRGAAA ARVKGGALAA ASSAGRERAC AHRPRGAKKA GIAATRLSRV IAIGASTGGT 190 200 210 220 230 240 EALRTVLQDM SGTPPPILIC QHMPEGFTAS FAARLDAICG IRVKEAEQGE PLHYGCAYVA 250 260 270 280 290 300 PGHSHLSLAA TGRLYVCRLE ASPPVNRHRP SVDVLFDSVA RLAGKRALGA ILTGMGKDGA 310 320 330 340 350 AGLLRMRASG ARTFAQDEPS CVVFGMPKEA IAMGAVDEIL PLARMGARLS EALQ

References

[1]

"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies." Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D. BMC Genomics 6:174-174(2005) [PubMed: 16336651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000085 Genomic DNA. Translation: ABC35113.1. Different initiation.
RefSeq	YP_438363.1. NC_007650.1.
3D structure databases
ProteinModelPortal	Q2T8Y6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T8Y6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3846303.
GenomeReviews	Gene locus BTH_II0161 in contig CP000085_GR.
KEGG	bte:BTH_II0161.
PATRIC	19297690. VBIBurTha36512_0176.
TIGR	BTH_II0161.
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
ProtClustDB	CLSK719951.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_II0161-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_BURTA

Accession

Primary (citable) accession number: Q2T8Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program