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Q2T7H6

- LEU3_BURTA

UniProt

Q2T7H6 - LEU3_BURTA

Protein

3-isopropylmalate dehydrogenase

Gene

leuB

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901SubstrateUniRule annotation
    Binding sitei100 – 1001SubstrateUniRule annotation
    Binding sitei128 – 1281SubstrateUniRule annotation
    Sitei135 – 1351Important for catalysisUniRule annotation
    Sitei190 – 1901Important for catalysisUniRule annotation
    Metal bindingi222 – 2221Magnesium or manganeseUniRule annotation
    Binding sitei222 – 2221SubstrateUniRule annotation
    Metal bindingi246 – 2461Magnesium or manganeseUniRule annotation
    Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi280 – 29213NADUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. 3-isopropylmalate dehydrogenase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciBTHA271848:GJMY-4018-MONOMER.
    UniPathwayiUPA00048; UER00072.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydrogenaseUniRule annotation (EC:1.1.1.85UniRule annotation)
    Alternative name(s):
    3-IPM-DHUniRule annotation
    Beta-IPM dehydrogenaseUniRule annotation
    Short name:
    IMDHUniRule annotation
    Gene namesi
    Name:leuBUniRule annotation
    Ordered Locus Names:BTH_II0674
    OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
    Taxonomic identifieri271848 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000001930: Chromosome II

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3553553-isopropylmalate dehydrogenasePRO_0000250109Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi271848.BTH_II0674.

    Structurei

    Secondary structure

    1
    355
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Helixi11 – 2515
    Beta strandi26 – 283
    Beta strandi31 – 344
    Helixi39 – 457
    Beta strandi46 – 494
    Helixi51 – 599
    Beta strandi60 – 678
    Helixi71 – 733
    Helixi78 – 803
    Helixi84 – 929
    Beta strandi97 – 1037
    Helixi106 – 1116
    Beta strandi112 – 1143
    Helixi116 – 1194
    Beta strandi123 – 1297
    Helixi133 – 1353
    Beta strandi141 – 1444
    Turni149 – 1524
    Beta strandi153 – 16311
    Helixi164 – 17916
    Turni180 – 1823
    Beta strandi183 – 1897
    Turni191 – 1933
    Helixi195 – 20814
    Beta strandi214 – 2207
    Helixi221 – 23010
    Helixi232 – 2343
    Beta strandi236 – 2405
    Helixi242 – 25615
    Helixi259 – 2613
    Beta strandi263 – 2675
    Beta strandi273 – 2775
    Turni283 – 2875
    Helixi294 – 30613
    Helixi311 – 32616
    Helixi332 – 3343
    Helixi344 – 35411

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4IWHX-ray1.75A/B1-355[»]
    ProteinModelPortaliQ2T7H6.
    SMRiQ2T7H6. Positions 2-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021112.
    KOiK00052.
    OMAiAKSTVEM.
    OrthoDBiEOG65N1BN.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_01033. LeuB_type1.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR024084. IsoPropMal-DH-like_dom.
    IPR004429. Isopropylmalate_DH.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00169. leuB. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2T7H6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIAVLPGDG IGPEIVNEAV KVLNALDEKF ELEHAPVGGA GYEASGHPLP    50
    DATLALAKEA DAILFGAVGD WKYDSLERAL RPEQAILGLR KHLELFANFR 100
    PAICYPQLVD ASPLKPELVA GLDILIVREL NGDIYFGQPR GVRAAPDGPF 150
    AGEREGFDTM RYSEPEVRRI AHVAFQAAQK RAKKLLSVDK SNVLETSQFW 200
    RDVMIDVSKE YADVELSHMY VDNAAMQLAK APKQFDVIVT GNMFGDILSD 250
    EASMLTGSIG MLPSASLDKN NKGLYEPSHG SAPDIAGKGI ANPLATILSA 300
    AMLLRYSLNR AEQADRIERA VKTVLEQGYR TGDIATPGCR QVGTAAMGDA 350
    VVAAL 355
    Length:355
    Mass (Da):38,242
    Last modified:January 24, 2006 - v1
    Checksum:iCE8C13AF0007C5E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000085 Genomic DNA. Translation: ABC35976.1.
    RefSeqiWP_009895843.1. NZ_CM000439.1.
    YP_438873.1. NC_007650.1.

    Genome annotation databases

    EnsemblBacteriaiABC35976; ABC35976; BTH_II0674.
    GeneIDi3845853.
    KEGGibte:BTH_II0674.
    PATRICi19298864. VBIBurTha36512_0762.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000085 Genomic DNA. Translation: ABC35976.1 .
    RefSeqi WP_009895843.1. NZ_CM000439.1.
    YP_438873.1. NC_007650.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4IWH X-ray 1.75 A/B 1-355 [» ]
    ProteinModelPortali Q2T7H6.
    SMRi Q2T7H6. Positions 2-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 271848.BTH_II0674.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC35976 ; ABC35976 ; BTH_II0674 .
    GeneIDi 3845853.
    KEGGi bte:BTH_II0674.
    PATRICi 19298864. VBIBurTha36512_0762.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021112.
    KOi K00052.
    OMAi AKSTVEM.
    OrthoDBi EOG65N1BN.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00072 .
    BioCyci BTHA271848:GJMY-4018-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_01033. LeuB_type1.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR024084. IsoPropMal-DH-like_dom.
    IPR004429. Isopropylmalate_DH.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00169. leuB. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
      Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
      BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

    Entry informationi

    Entry nameiLEU3_BURTA
    AccessioniPrimary (citable) accession number: Q2T7H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3