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Q2T7H6

- LEU3_BURTA

UniProt

Q2T7H6 - LEU3_BURTA

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Protein

3-isopropylmalate dehydrogenase

Gene

leuB

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901SubstrateUniRule annotation
Binding sitei100 – 1001SubstrateUniRule annotation
Binding sitei128 – 1281SubstrateUniRule annotation
Sitei135 – 1351Important for catalysisUniRule annotation
Sitei190 – 1901Important for catalysisUniRule annotation
Metal bindingi222 – 2221Magnesium or manganeseUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Metal bindingi246 – 2461Magnesium or manganeseUniRule annotation
Metal bindingi250 – 2501Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi280 – 29213NADUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. 3-isopropylmalate dehydrogenase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBTHA271848:GJMY-4018-MONOMER.
UniPathwayiUPA00048; UER00072.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydrogenaseUniRule annotation (EC:1.1.1.85UniRule annotation)
Alternative name(s):
3-IPM-DHUniRule annotation
Beta-IPM dehydrogenaseUniRule annotation
Short name:
IMDHUniRule annotation
Gene namesi
Name:leuBUniRule annotation
Ordered Locus Names:BTH_II0674
OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000001930: Chromosome II

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3553553-isopropylmalate dehydrogenasePRO_0000250109Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi271848.BTH_II0674.

Structurei

Secondary structure

1
355
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98Combined sources
Helixi11 – 2515Combined sources
Beta strandi26 – 283Combined sources
Beta strandi31 – 344Combined sources
Helixi39 – 457Combined sources
Beta strandi46 – 494Combined sources
Helixi51 – 599Combined sources
Beta strandi60 – 678Combined sources
Helixi71 – 733Combined sources
Helixi78 – 803Combined sources
Helixi84 – 929Combined sources
Beta strandi97 – 1037Combined sources
Helixi106 – 1116Combined sources
Beta strandi112 – 1143Combined sources
Helixi116 – 1194Combined sources
Beta strandi123 – 1297Combined sources
Helixi133 – 1353Combined sources
Beta strandi141 – 1444Combined sources
Turni149 – 1524Combined sources
Beta strandi153 – 16311Combined sources
Helixi164 – 17916Combined sources
Turni180 – 1823Combined sources
Beta strandi183 – 1897Combined sources
Turni191 – 1933Combined sources
Helixi195 – 20814Combined sources
Beta strandi214 – 2207Combined sources
Helixi221 – 23010Combined sources
Helixi232 – 2343Combined sources
Beta strandi236 – 2405Combined sources
Helixi242 – 25615Combined sources
Helixi259 – 2613Combined sources
Beta strandi263 – 2675Combined sources
Beta strandi273 – 2775Combined sources
Turni283 – 2875Combined sources
Helixi294 – 30613Combined sources
Helixi311 – 32616Combined sources
Helixi332 – 3343Combined sources
Helixi344 – 35411Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IWHX-ray1.75A/B1-355[»]
ProteinModelPortaliQ2T7H6.
SMRiQ2T7H6. Positions 2-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021112.
KOiK00052.
OMAiAKSTVEM.
OrthoDBiEOG65N1BN.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_01033. LeuB_type1.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00169. leuB. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2T7H6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIAVLPGDG IGPEIVNEAV KVLNALDEKF ELEHAPVGGA GYEASGHPLP
60 70 80 90 100
DATLALAKEA DAILFGAVGD WKYDSLERAL RPEQAILGLR KHLELFANFR
110 120 130 140 150
PAICYPQLVD ASPLKPELVA GLDILIVREL NGDIYFGQPR GVRAAPDGPF
160 170 180 190 200
AGEREGFDTM RYSEPEVRRI AHVAFQAAQK RAKKLLSVDK SNVLETSQFW
210 220 230 240 250
RDVMIDVSKE YADVELSHMY VDNAAMQLAK APKQFDVIVT GNMFGDILSD
260 270 280 290 300
EASMLTGSIG MLPSASLDKN NKGLYEPSHG SAPDIAGKGI ANPLATILSA
310 320 330 340 350
AMLLRYSLNR AEQADRIERA VKTVLEQGYR TGDIATPGCR QVGTAAMGDA

VVAAL
Length:355
Mass (Da):38,242
Last modified:January 24, 2006 - v1
Checksum:iCE8C13AF0007C5E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000085 Genomic DNA. Translation: ABC35976.1.
RefSeqiWP_009895843.1. NZ_CM000439.1.
YP_438873.1. NC_007650.1.

Genome annotation databases

EnsemblBacteriaiABC35976; ABC35976; BTH_II0674.
GeneIDi3845853.
KEGGibte:BTH_II0674.
PATRICi19298864. VBIBurTha36512_0762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000085 Genomic DNA. Translation: ABC35976.1 .
RefSeqi WP_009895843.1. NZ_CM000439.1.
YP_438873.1. NC_007650.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IWH X-ray 1.75 A/B 1-355 [» ]
ProteinModelPortali Q2T7H6.
SMRi Q2T7H6. Positions 2-355.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 271848.BTH_II0674.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC35976 ; ABC35976 ; BTH_II0674 .
GeneIDi 3845853.
KEGGi bte:BTH_II0674.
PATRICi 19298864. VBIBurTha36512_0762.

Phylogenomic databases

eggNOGi COG0473.
HOGENOMi HOG000021112.
KOi K00052.
OMAi AKSTVEM.
OrthoDBi EOG65N1BN.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00072 .
BioCyci BTHA271848:GJMY-4018-MONOMER.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
HAMAPi MF_01033. LeuB_type1.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00169. leuB. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
    Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
    BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Entry informationi

Entry nameiLEU3_BURTA
AccessioniPrimary (citable) accession number: Q2T7H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: January 24, 2006
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3