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Q2T7H6

- LEU3_BURTA

UniProt

Q2T7H6 - LEU3_BURTA

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Protein

3-isopropylmalate dehydrogenase

Gene
leuB, BTH_II0674
Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901Substrate By similarity
Binding sitei100 – 1001Substrate By similarity
Binding sitei128 – 1281Substrate By similarity
Sitei135 – 1351Important for catalysis By similarity
Sitei190 – 1901Important for catalysis By similarity
Metal bindingi222 – 2221Magnesium or manganese By similarity
Binding sitei222 – 2221Substrate By similarity
Metal bindingi246 – 2461Magnesium or manganese By similarity
Metal bindingi250 – 2501Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi280 – 29213NAD By similarityAdd
BLAST

GO - Molecular functioni

  1. 3-isopropylmalate dehydrogenase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBTHA271848:GJMY-4018-MONOMER.
UniPathwayiUPA00048; UER00072.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydrogenase (EC:1.1.1.85)
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name:
IMDH
Gene namesi
Name:leuB
Ordered Locus Names:BTH_II0674
OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000001930: Chromosome II

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3553553-isopropylmalate dehydrogenaseUniRule annotationPRO_0000250109Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi271848.BTH_II0674.

Structurei

Secondary structure

1
355
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98
Helixi11 – 2515
Beta strandi26 – 283
Beta strandi31 – 344
Helixi39 – 457
Beta strandi46 – 494
Helixi51 – 599
Beta strandi60 – 678
Helixi71 – 733
Helixi78 – 803
Helixi84 – 929
Beta strandi97 – 1037
Helixi106 – 1116
Beta strandi112 – 1143
Helixi116 – 1194
Beta strandi123 – 1297
Helixi133 – 1353
Beta strandi141 – 1444
Turni149 – 1524
Beta strandi153 – 16311
Helixi164 – 17916
Turni180 – 1823
Beta strandi183 – 1897
Turni191 – 1933
Helixi195 – 20814
Beta strandi214 – 2207
Helixi221 – 23010
Helixi232 – 2343
Beta strandi236 – 2405
Helixi242 – 25615
Helixi259 – 2613
Beta strandi263 – 2675
Beta strandi273 – 2775
Turni283 – 2875
Helixi294 – 30613
Helixi311 – 32616
Helixi332 – 3343
Helixi344 – 35411

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IWHX-ray1.75A/B1-355[»]
ProteinModelPortaliQ2T7H6.
SMRiQ2T7H6. Positions 2-355.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021112.
KOiK00052.
OMAiAKSTVEM.
OrthoDBiEOG65N1BN.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_01033. LeuB_type1.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00169. leuB. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2T7H6-1 [UniParc]FASTAAdd to Basket

« Hide

MKIAVLPGDG IGPEIVNEAV KVLNALDEKF ELEHAPVGGA GYEASGHPLP    50
DATLALAKEA DAILFGAVGD WKYDSLERAL RPEQAILGLR KHLELFANFR 100
PAICYPQLVD ASPLKPELVA GLDILIVREL NGDIYFGQPR GVRAAPDGPF 150
AGEREGFDTM RYSEPEVRRI AHVAFQAAQK RAKKLLSVDK SNVLETSQFW 200
RDVMIDVSKE YADVELSHMY VDNAAMQLAK APKQFDVIVT GNMFGDILSD 250
EASMLTGSIG MLPSASLDKN NKGLYEPSHG SAPDIAGKGI ANPLATILSA 300
AMLLRYSLNR AEQADRIERA VKTVLEQGYR TGDIATPGCR QVGTAAMGDA 350
VVAAL 355
Length:355
Mass (Da):38,242
Last modified:January 24, 2006 - v1
Checksum:iCE8C13AF0007C5E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000085 Genomic DNA. Translation: ABC35976.1.
RefSeqiWP_009895843.1. NZ_CM000439.1.
YP_438873.1. NC_007650.1.

Genome annotation databases

EnsemblBacteriaiABC35976; ABC35976; BTH_II0674.
GeneIDi3845853.
KEGGibte:BTH_II0674.
PATRICi19298864. VBIBurTha36512_0762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000085 Genomic DNA. Translation: ABC35976.1 .
RefSeqi WP_009895843.1. NZ_CM000439.1.
YP_438873.1. NC_007650.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IWH X-ray 1.75 A/B 1-355 [» ]
ProteinModelPortali Q2T7H6.
SMRi Q2T7H6. Positions 2-355.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 271848.BTH_II0674.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC35976 ; ABC35976 ; BTH_II0674 .
GeneIDi 3845853.
KEGGi bte:BTH_II0674.
PATRICi 19298864. VBIBurTha36512_0762.

Phylogenomic databases

eggNOGi COG0473.
HOGENOMi HOG000021112.
KOi K00052.
OMAi AKSTVEM.
OrthoDBi EOG65N1BN.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00072 .
BioCyci BTHA271848:GJMY-4018-MONOMER.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
HAMAPi MF_01033. LeuB_type1.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00169. leuB. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
    Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
    BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Entry informationi

Entry nameiLEU3_BURTA
AccessioniPrimary (citable) accession number: Q2T7H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: January 24, 2006
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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