Reviewed, UniProtKB/Swiss-Prot Q2T6R3 (GLGB_BURTA)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	BTH_II0939

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000260640
Regions
	Region	1 – 82	82	Unknown HAMAP MF_00685
	Region	83 – 834	752	1,4-alpha-glucan-branching enzyme HAMAP MF_00685
Sites
	Active site	406	1	By similarity
	Active site	441	1	By similarity
	Active site	446	1	By similarity
	Active site	509	1	By similarity
	Active site	511	1	By similarity
	Active site	565	1	By similarity
	Active site	632	1	By similarity
	Active site	633	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T6R3-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 59BCF5695BFA9C18	FASTA	834	92,904
	10 20 30 40 50 60 MTGIGARTAW RNAVGRRPRN ARRVSRIAYR VSRVACRVSR IAYRVSRIAY RVSRVAWRNV 70 80 90 100 110 120 APAARRAHRP FPAVIHIASG IPLPTEPLPA PPVVVDRPAS PDDAALIAPD DLARLLRGEH 130 140 150 160 170 180 DDPFAVLGIH AESSARDVVV RCLLPGAARV ELIDAASART LATLSPVGSG ELHAIRLPAP 190 200 210 220 230 240 GPLRYRLRAH YADTVRDLDD PYACTPWLGS LDCHLLARGE HRDAYRRLGA HPCVHDGLEG 250 260 270 280 290 300 TAFALWAPNA SCVSVVGSFN GWDARVHAMR KRIECGVWEL FVPGVGCGAL YKFALRTRDG 310 320 330 340 350 360 DRLLKADPYA RRTEAPPRTA SRICAPSAFG WRDDAWMRER AAAQSAHAPI AIYEVHLDSW 370 380 390 400 410 420 RRHPDGRAYS YDELADALIP YVAALGFTHV ELLPIAEYPF AGSWGYQPVS LFAPSARWGE 430 440 450 460 470 480 PDALRRFVER CHLAGLGVLL DWVPAHFPQD AHGLARFDGT HLYEHEDRRV GLHRGWNTLV 490 500 510 520 530 540 YNLGRHEVAN FLIANALYWL REFHFDGLRV DAVASMLYLD YDRDDGQWLP NVHGGRENLE 550 560 570 580 590 600 AVAFLRRLNE TVHADAPQGA ITIAEESTAW PMVSAPVAAG GLGFDFKWNM GWMNDTLSFM 610 620 630 640 650 660 RVDPIHRRFH LDRLTFGLLY AWSEQFVLAL SHDEVVHAKG SLLAKMPGDA WQRHANLRLY 670 680 690 700 710 720 LAFQYAHPGK KLLFMGSEFG QEREWNHDRE LDWARLADPA SAGVRRLVGD LNRLYRRRGC 730 740 750 760 770 780 LHRRDADSRG FRWIDCADSH QTVIAWRRIG DAPDDFVVVV CNFTPQPRTG YRIGVPAAGF 790 800 810 820 830 YRELLNSDAA DYGGSGLGNL GGVSSEPVPM HGEPHSLSLL LPPLAALVFA APGH

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000085 Genomic DNA. Translation: ABC35639.1.
RefSeq	YP_439136.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T6R3.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3845029.
GenomeReviews	Gene locus BTH_II0939 in contig CP000085_GR.
KEGG	bte:BTH_II0939.
TIGR	BTH_II0939.
Phylogenomic databases
HOGENOM	Q2T6R3.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_II0939-MON.
Family and domain databases
HAMAP	MF_00685. Fused. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURTA

Accession

Primary (citable) accession number: Q2T6R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 24, 2006
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)