Reviewed, UniProtKB/Swiss-Prot Q2T6D0 (BETA_BURTA)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	BTH_II1072

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Choline dehydrogenase HAMAP MF_00750		PRO_0000258922
Regions
	Nucleotide binding	7 – 36	30	FAD Probable
Sites
	Active site	474	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T6D0-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 4BFCAD32183064FD	FASTA	565	62,691
	10 20 30 40 50 60 MTTREFDYII CGAGSAGNVL ATRLTEDPDV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPHMNNRRM ECGRGKGLGG SSLINGMCYI RGNALDYDNW STHKGLEDWT 130 140 150 160 170 180 YLDCLPYFRK AETRDVGPND YHGGDGPVSV TTSKPGVNPL FEAMVEAGVQ AGYPRTDDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTPRGRR ASTARGYLDQ ARARPNLEIV THALADRILF SGKRATGVTF 250 260 270 280 290 300 LHGSARVTAH ARREVLVCSG AIASPQLLQR SGVGPGEWLR ELDIPVVLDL PGVGRNLQDH 310 320 330 340 350 360 LEMYIQFECK EPVSLYPALK WWNQPKIGLD WMINGTGLGA SNHFEAGGFI RTRDDDLWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGSNAIEM HGFQAHVGSM RSPSRGRVKL KSRDPNAHPS ILFNYMAEAL 430 440 450 460 470 480 DWREFRDAIR ATREIMHQPA LDRFRGRELN PGADLKSDNE LDAFVRARAE TAFHPSCSCK 490 500 510 520 530 540 MGYDDMAVVD NEGRVHGIDG LRVVDASIMP IITTGNLNAP TIMIAEKIAD KIRKRKPLER 550 560 SNARYYVANG APARGGKPAR APAAV

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000085 Genomic DNA. Translation: ABC35233.1.
RefSeq	YP_439269.1.
3D structure databases
SMR	Q2T6D0. Positions 1-532.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T6D0.
Genome annotation databases
GeneID	3846715.
GenomeReviews	Gene locus BTH_II1072 in contig CP000085_GR.
KEGG	bte:BTH_II1072.
TIGR	BTH_II1072.
Phylogenomic databases
eggNOG	COG2303.
HOGENOM	HBG734713.
OMA	RTVTPHG.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_II1072-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BURTA

Accession

Primary (citable) accession number: Q2T6D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 24, 2006
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)