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Q2T4T8 (MDH2_BURTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase 2
EC=1.1.1.37
Gene names
Name:mdh2
Ordered Locus Names:BTH_II1617
OrganismBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]
Taxonomic identifier271848 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group ›

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Malate dehydrogenase 2 HAMAP-Rule MF_01517
PRO_0000292370

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding130 – 1323NAD By similarity

Sites

Active site1881Proton acceptor By similarity
Binding site931Substrate By similarity
Binding site991Substrate By similarity
Binding site1061NAD By similarity
Binding site1131NAD By similarity
Binding site1321Substrate By similarity
Binding site1631Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2T4T8 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 7771577BC065D687

FASTA32936,101
        10         20         30         40         50         60 
MNTEAKRIAV SGAAGQIAYS LLFRIAQGDL LGEDQPVILQ LLDLPQAYGA VQGVVMELQD 

        70         80         90        100        110        120 
CAFPLLKEIQ VATDPHAAFL NADYAFLVGS KPRTKGMERR DLLAENAAIF RTQGRALNEA 

       130        140        150        160        170        180 
ASRDVKVLVV GNPANTNASI LRRFAPDLPD DAISAMIRLD HNRAVSMLAQ RCNVDVDSIA 

       190        200        210        220        230        240 
DMVVWGNHSP TMFPDYRHAR IGRRLVKDLI NDENWYRESF IPKVAQRGTA IIEARGASSA 

       250        260        270        280        290        300 
ASAANAAIDQ MRDWIFGSDG RWVSMSVPSD GSYGIAPGLM FGVPVICDGG RYERVKDIGI 

       310        320 
DAFARQRIDL SVRELQEEAD VVNRLFADR

« Hide

References

[1]"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000085 Genomic DNA. Translation: ABC35074.1.
RefSeqYP_439811.1. NC_007650.1.

3D structure databases

HSSPHSSP built from PDB template 1WZE based on UniProtKB P10584.
ProteinModelPortalQ2T4T8.
SMRQ2T4T8. Positions 5-325.
ModBaseSearch...

Protein-protein interaction databases

STRING271848.BTH_II1617.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC35074; ABC35074; BTH_II1617.
GeneID3844557.
KEGGbte:BTH_II1617.
PATRIC19300910. VBIBurTha36512_1783.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000220953.
KOK00024.
OMAEWALLIG.
ProtClustDBPRK05442.

Enzyme and pathway databases

BioCycBTHA271848:GJMY-4960-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH2_BURTA
AccessionPrimary (citable) accession number: Q2T4T8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: January 24, 2006
Last modified: May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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