ID   ASSY_BURTA              Reviewed;         446 AA.
AC   Q2T1W2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Argininosuccinate synthase;
DE            EC=6.3.4.5;
DE   AltName: Full=Citrulline--aspartate ligase;
GN   Name=argG; OrderedLocusNames=BTH_I0279;
OS   Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 /
OS   CIP 106301).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R.,
RA   Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D.,
RA   Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J.,
RA   Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D.,
RA   Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P.,
RA   Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F.,
RA   Roberts K., Hatch B., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-citrulline + L-aspartate = AMP +
CC       diphosphate + N(omega)-(L-arginino)succinate.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-
CC       arginine from L-ornithine and carbamoyl phosphate: step 2/3.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the argininosuccinate synthase family. Type
CC       2 subfamily.
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DR   EMBL; CP000086; ABC37394.1; -; Genomic_DNA.
DR   RefSeq; YP_440837.1; -.
DR   SMR; Q2T1W2; 2-444.
DR   GeneID; 3848636; -.
DR   GenomeReviews; CP000086_GR; BTH_I0279.
DR   KEGG; bte:BTH_I0279; -.
DR   TIGR; BTH_I0279; -.
DR   HOGENOM; Q2T1W2; -.
DR   OMA; Q2T1W2; GGRKEMS.
DR   BioCyc; BTHA271848:BTH_I0279-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004055; F:argininosuccinate synthase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00581; -; 1.
DR   InterPro; IPR001518; Arginosuc_synth.
DR   InterPro; IPR018223; Arginosuc_synth_CS.
DR   PANTHER; PTHR11587; Arginosuc_synth; 1.
DR   Pfam; PF00764; Arginosuc_synth; 2.
DR   TIGRFAMs; TIGR00032; argG; 1.
DR   PROSITE; PS00564; ARGININOSUCCIN_SYN_1; 1.
DR   PROSITE; PS00565; ARGININOSUCCIN_SYN_2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding;
KW   Complete proteome; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN         1    446       Argininosuccinate synthase.
FT                                /FTId=PRO_1000025418.
FT   NP_BIND      17     25       ATP (By similarity).
FT   BINDING      43     43       ATP (By similarity).
FT   BINDING      99     99       Citrulline (By similarity).
FT   BINDING     129    129       ATP; via amide nitrogen (By similarity).
FT   BINDING     131    131       Aspartate (By similarity).
FT   BINDING     131    131       ATP (By similarity).
FT   BINDING     135    135       Aspartate (By similarity).
FT   BINDING     135    135       Citrulline (By similarity).
FT   BINDING     136    136       Aspartate (By similarity).
FT   BINDING     136    136       ATP (By similarity).
FT   BINDING     139    139       Citrulline (By similarity).
FT   BINDING     192    192       Citrulline (By similarity).
FT   BINDING     194    194       ATP (By similarity).
FT   BINDING     201    201       Citrulline (By similarity).
FT   BINDING     203    203       Citrulline (By similarity).
FT   BINDING     280    280       Citrulline (By similarity).
SQ   SEQUENCE   446 AA;  49724 MW;  DB3CB1F6F54C217D CRC64;
     MTTILENLPA GQKVGIAFSG GLDTSAALHW MRIKGAVPYA YTANLGQPDE DDYDAIPKRA
     IQYGAEGARL IDCRAQLVAE GIAALQCGAF HISTAGVTYF NTTPIGRAVT GTMLVAAMKE
     DGVNIWGDGS TYKGNDIERF YRYGLLVNPD LKIYKPWLDQ QFIDELGGRA EMSEFMRQAG
     FEYKMSAEKA YSTDSNLLGA THEAKDLESL ESGIKIVNPI MGVAFWRDDV KIDKEAVTVR
     FEEGRPVALN GVEYKDAVEL LLEANRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGLALL
     YIAYERLVTG IHNEDTIEQY RENGRRLGRL LYQGRWFDPQ AIMLRETAQR WVARAVTGEV
     TVELRRGNDY SILATRSPNL TYQPERLSME KVQSTFSPRD RIGQLTMRNL DITDTRDKLR
     IYSEVGLLTP GESSALPKLK EDESGN
//