Q2T1Q3 (BIOD_BURTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	BTH_I0338

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302494
Regions
	Nucleotide binding	118 – 121	4	ATP By similarity
	Nucleotide binding	178 – 179	2	ATP By similarity
Sites
	Metal binding	15	1	Magnesium 1 By similarity
	Metal binding	19	1	Magnesium 2 By similarity
	Metal binding	57	1	Magnesium 2 By similarity
	Metal binding	118	1	Magnesium 2 By similarity
	Binding site	57	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T1Q3 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 71BA9D43748EA278	FASTA	240	24,955
	10 20 30 40 50 60 MSAPLSLFVT GTDTEIGKTF VSTALLHGFA RAGLRAAAMK PIAAGAYQRD GVWRNEDADQ 70 80 90 100 110 120 LDAAANVALP ATIRTPFLLK AAAAPHIAAA REGVALDIDT IVGAHRRACE RADVVVVEGV 130 140 150 160 170 180 GGFRVPLAGT RDTADLAVAL GLPVVLVVGV RLGCISHALL TADAIAARGL AIAGWVANRV 190 200 210 220 230 240 DAAMRFADDN VDTLRAWLER EHGAPLLGDI AHMSPPSPEA ASRSLDVNRL LGALRETAPR

References

[1]

"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies." Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D. BMC Genomics 6:174-174(2005) [PubMed: 16336651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000086 Genomic DNA. Translation: ABC39482.1.
RefSeq	YP_440896.1. NC_007651.1.
3D structure databases
ProteinModelPortal	Q2T1Q3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T1Q3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3848467.
GenomeReviews	Gene locus BTH_I0338 in contig CP000086_GR.
KEGG	bte:BTH_I0338.
PATRIC	19303364. VBIBurTha36512_3001.
TIGR	BTH_I0338.
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	HRRACER.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I0338-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURTA

Accession

Primary (citable) accession number: Q2T1Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program