Reviewed, UniProtKB/Swiss-Prot Q2T1P5 (ACEK_BURTA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	BTH_I0346

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000259148
Regions
	Nucleotide binding	327 – 333	7	ATP By similarity
Sites
	Active site	383	1	By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T1P5-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: E6C0B30A99CEDF29	FASTA	603	70,047
	10 20 30 40 50 60 MNHFPKLLSS QIGFDVAQTI LENFDRHYRI FREAAVEAKD LFERSDWHGL QRLARERITS 70 80 90 100 110 120 YDDRVRECVE LLEDEYDAEN IDHDVWPQIK LHYIGLLTSH RQPECAETFF NSVCCKILHR 130 140 150 160 170 180 AYFNNDFIFV RPAISTEYIE NDEPAAKPTY RAYYPGSEGL AATLERIVTN FQLNPPFEDL 190 200 210 220 230 240 ERDIACIMQA IHDEFGAFDE AVNFQIHVLS SLFYRNKTAY IVGRIINGDR VLPFAVPIRH 250 260 270 280 290 300 ARAGVLALDT VLLRRDQLKI IFSFSHSYFL VDMNVPSAYV QFLRSIMPGK PKAEIYTSVG 310 320 330 340 350 360 LQKQGKNLFY RDLLHHLSHS SDRFIIAPGI KGLVMLVFTL PSFPYVFKMI KDHFPPPKET 370 380 390 400 410 420 TREQIMDKYL LVKRHDRLGR MADTLEYSSV ALPLARLDDA LVRELEKEVP SLIEYEGDSL 430 440 450 460 470 480 VIKHLYIERR MVPLNLYLQN GSDAEIEHGV REYGNAVKEL IQANIFPGDM LYKNFGVTRH 490 500 510 520 530 540 GRVVFYDYDE IEYLTDCNVR RVPPPRNDED EMSGEPWYTV GPHDIFPETY APFLLGDPRV 550 560 570 580 590 600 REHFLAHHAD FFDPQLWQDS KDRLLRGELP DFFAYEPALR FCLRYPERFA PGDAAEDGKR AAA

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000086 Genomic DNA. Translation: ABC36516.1.
RefSeq	YP_440904.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T1P5.
Genome annotation databases
GeneID	3847164.
GenomeReviews	Gene locus BTH_I0346 in contig CP000086_GR.
KEGG	bte:BTH_I0346.
TIGR	BTH_I0346.
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I0346-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_BURTA

Accession

Primary (citable) accession number: Q2T1P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 24, 2006
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)