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Q2T1K5

- LIPA_BURTA

UniProt

Q2T1K5 - LIPA_BURTA

Protein

Lipoyl synthase

Gene

lipA

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi106 – 1061Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi109 – 1091Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBTHA271848:GJMY-386-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:BTH_I0386
    OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
    Taxonomic identifieri271848 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000001930: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Lipoyl synthasePRO_1000012204Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi271848.BTH_I0386.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2T1K5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2T1K5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDLTATPAP AEPAASAYDP TAKQKAQAKT ARIPIKIVPI EKLKKPEWIR    50
    VKAATGSSRF NEIKTILREH NLHTVCEEAS CPNIGECFGK GTATFMIMGD 100
    KCTRRCPFCD VGHGRPDPLD ADEPKNLART IAALKLKYVV ITSVDRDDLR 150
    DGGAGHFVEC IREVREQSPA TRIEILTPDF RGRLDRALEI LNAAPPDVMN 200
    HNLETVPRLY KEARPGSDYA HSLKLLKDFK ALHPDVATKS GLMVGLGETT 250
    DEILQVMRDL RAHDVDMLTI GQYLQPSEHH LPVREYVHPD TFKMYEEEAY 300
    KMGFTHAAVG AMVRSSYHAD LQAHGAGVV 329
    Length:329
    Mass (Da):36,487
    Last modified:January 24, 2006 - v1
    Checksum:i2098694EAFC3E21A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000086 Genomic DNA. Translation: ABC38383.1.
    RefSeqiYP_440944.1. NC_007651.1.

    Genome annotation databases

    EnsemblBacteriaiABC38383; ABC38383; BTH_I0386.
    GeneIDi3849603.
    KEGGibte:BTH_I0386.
    PATRICi19303478. VBIBurTha36512_3058.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000086 Genomic DNA. Translation: ABC38383.1 .
    RefSeqi YP_440944.1. NC_007651.1.

    3D structure databases

    ProteinModelPortali Q2T1K5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 271848.BTH_I0386.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC38383 ; ABC38383 ; BTH_I0386 .
    GeneIDi 3849603.
    KEGGi bte:BTH_I0386.
    PATRICi 19303478. VBIBurTha36512_3058.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci BTHA271848:GJMY-386-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
      Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
      BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

    Entry informationi

    Entry nameiLIPA_BURTA
    AccessioniPrimary (citable) accession number: Q2T1K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3