Q2T144 (QUEF_BURTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	BTH_I0548

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000247706

Sequences

	Sequence		Length	Mass (Da)
	Q2T144 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 4D0F932EAF8319E4	FASTA	274	30,451
	10 20 30 40 50 60 MNPEHSPLGK TTVYANQYDA SLLFPIPRAG AREQIGIGAP LPFFGTDIWN AYELSWLNAR 70 80 90 100 110 120 GKPQVAIATF YVPAESPNIV ESKSFKLYLG SFAQTAFESI DAVRDALKRD VSAACGASVT 130 140 150 160 170 180 VRLATPAEFR KLQMDELDGL SLDRLDLDAD VYETDPSFLT ASHDEAPVEE TLVTDLLKSN 190 200 210 220 230 240 CPVTGQPDWG SVQIHYVGAP IDHAGLLRYI ISFRNHTGFH EQCVERIFID ILRACKPVKL 250 260 270 AVYARYTRRG GLDINPFRTN YNQPMPDNAR TARQ

References

[1]

"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies." Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D. BMC Genomics 6:174-174(2005) [PubMed: 16336651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000086 Genomic DNA. Translation: ABC38829.1.
RefSeq	YP_441105.1. NC_007651.1.
3D structure databases
ProteinModelPortal	Q2T144.
SMR	Q2T144. Positions 14-274.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T144.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3849505.
GenomeReviews	Gene locus BTH_I0548 in contig CP000086_GR.
KEGG	bte:BTH_I0548.
PATRIC	19303834. VBIBurTha36512_3235.
TIGR	BTH_I0548.
Phylogenomic databases
eggNOG	COG2904.
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I0548-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BURTA

Accession

Primary (citable) accession number: Q2T144

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program