Reviewed, UniProtKB/Swiss-Prot Q2T0Z3 (GLPK_BURTA)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BTH_I0599

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000020713
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T0Z3-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: E1E14456E8E4F2F6	FASTA	500	54,710
	10 20 30 40 50 60 MQDQYILALD QGTTSSRAML FDRQGNIVSI AQKEFEQIYP RPGWVEHDPQ EIWSTQAGVA 70 80 90 100 110 120 AEAVTRVGLN GTSIAAIGIT NQRETTIVWD RETGHPIYNA IVWQDRRTAD FCDQLKAQGL 130 140 150 160 170 180 SEKVRAKTGL PIDSYFSATK IRWILDNVEG AREKARQGKL AFGTVDSWLV WNFTKHELHV 190 200 210 220 230 240 TDVTNASRTM LFNIHTLDWD DELLDALEIP RSMLPQVRAS SEIYGPTKTT VFASKIPLAG 250 260 270 280 290 300 IAGDQQAALF GQMCTSSGMV KNTYGTGCFL MMNTGETPIE SQNNLVTTIA WQVDGKVNYA 310 320 330 340 350 360 LEGSIFIAGA VVQWLRDGLG IIKSASEIEA LAAGVPHTDG VYLVPAFAGL GAPHWNARAR 370 380 390 400 410 420 GSLFGVTRGT TSAHLARAAL DSIAYQSLDV LKAMEADSGI RIGELRVDGG ASANNLLMQF 430 440 450 460 470 480 QADLLGVDAV RPRVTETTAL GAAYLAGLAI GYWKNVDELH SQWQLERRFA PSMQREQVSS 490 500 CLCGWQRAVR AAKAWADDTQ

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC37809.1.
RefSeq	YP_441156.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3849032.
GenomeReviews	Gene locus BTH_I0599 in contig CP000086_GR.
KEGG	bte:BTH_I0599.
TIGR	BTH_I0599.
Phylogenomic databases
HOGENOM	Q2T0Z3.
OMA	Q2T0Z3. IAQREFT.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I0599-MON.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BURTA

Accession

Primary (citable) accession number: Q2T0Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)