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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. N5-carboxyaminoimidazole ribonucleotide synthase (purK)
  2. N5-carboxyaminoimidazole ribonucleotide mutase (purE)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113ATPUniRule annotation1
Binding sitei156ATPUniRule annotation1
Binding sitei199ATPUniRule annotation1
Binding sitei222ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi161 – 167ATPUniRule annotation7
Nucleotide bindingi191 – 194ATPUniRule annotation4
Nucleotide bindingi277 – 278ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation, LyaseImported

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotationImported
Ordered Locus Names:BTH_I0668Imported
ORF Names:DR63_2444Imported
OrganismiBurkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)Imported
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000001930 Componenti: Chromosome I

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IZOX-ray1.85A1-398[»]
ProteinModelPortaliQ2T0S5.
SMRiQ2T0S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini117 – 307ATP-graspInterPro annotationAdd BLAST191

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotationSAAS annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiPOG091H069C.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2T0S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALPNPNSP ILPGAWLGMV GGGQLGRMFC FAAQAMGYRV AVLDPDPTSP
60 70 80 90 100
AGAVADKHLR AAYDDEAALA ELAQLCDAVS TEFENVPAAS LDFLAQSTFV
110 120 130 140 150
APAGRCVAIA QDRIAEKRFI AASGVPVAPH VVIESAAQLA ALADADLAAV
160 170 180 190 200
LPGILKTARL GYDGKGQVRV ATAQEARDAY GSLGGVPCVL EKRLPLKYEV
210 220 230 240 250
SALIARGANG ASAVFPLAQN THHGGILSLS VVPAPAASDA LVRDAQQAAA
260 270 280 290 300
RIADSLDYVG VLCVEFFVLE DGSLVANEMA PRPHNSGHYT VDACETSQFE
310 320 330 340 350
QQVRAMTRLP LGSTRQHSPA AMLNVLGDVW FASGASGEPV TPPWDQVAAM
360 370 380 390
PTARLHLYGK EEARVGRKMG HVNFTAATLD EAVAGATACA RLLRIPLD
Length:398
Mass (Da):41,463
Last modified:January 24, 2006 - v1
Checksum:i245ED46131BE00EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37891.1.
CP008785 Genomic DNA. Translation: AIP25373.1.
RefSeqiWP_009892765.1. NZ_CM000438.1.

Genome annotation databases

EnsemblBacteriaiABC37891; ABC37891; BTH_I0668.
KEGGibte:BTH_I0668.
PATRICi19304101. VBIBurTha36512_3367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37891.1.
CP008785 Genomic DNA. Translation: AIP25373.1.
RefSeqiWP_009892765.1. NZ_CM000438.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IZOX-ray1.85A1-398[»]
ProteinModelPortaliQ2T0S5.
SMRiQ2T0S5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC37891; ABC37891; BTH_I0668.
KEGGibte:BTH_I0668.
PATRICi19304101. VBIBurTha36512_3367.

Phylogenomic databases

HOGENOMiHOG000034029.
KOiK01589.
OMAiDSPCGQV.
OrthoDBiPOG091H069C.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ2T0S5_BURTA
AccessioniPrimary (citable) accession number: Q2T0S5
Entry historyi
Integrated into UniProtKB/TrEMBL: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.