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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 5-(carboxyamino)imidazole ribonucleotide synthase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: InterPro
  4. phosphoribosylaminoimidazole carboxylase activity Source: InterPro

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation, LyaseImported

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHA271848:GJMY-668-MONOMER.
UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotationImported
Ordered Locus Names:BTH_I0668Imported
ORF Names:DR63_2444Imported
OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)Imported
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000001930: Chromosome I

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi271848.BTH_I0668.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IZOX-ray1.85A1-398[»]
ProteinModelPortaliQ2T0S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains ATP-grasp domain.SAAS annotation

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034029.
KOiK01589.
OrthoDBiEOG6QZMX7.

Sequencei

Sequence statusi: Complete.

Q2T0S5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTALPNPNSP ILPGAWLGMV GGGQLGRMFC FAAQAMGYRV AVLDPDPTSP
60 70 80 90 100
AGAVADKHLR AAYDDEAALA ELAQLCDAVS TEFENVPAAS LDFLAQSTFV
110 120 130 140 150
APAGRCVAIA QDRIAEKRFI AASGVPVAPH VVIESAAQLA ALADADLAAV
160 170 180 190 200
LPGILKTARL GYDGKGQVRV ATAQEARDAY GSLGGVPCVL EKRLPLKYEV
210 220 230 240 250
SALIARGANG ASAVFPLAQN THHGGILSLS VVPAPAASDA LVRDAQQAAA
260 270 280 290 300
RIADSLDYVG VLCVEFFVLE DGSLVANEMA PRPHNSGHYT VDACETSQFE
310 320 330 340 350
QQVRAMTRLP LGSTRQHSPA AMLNVLGDVW FASGASGEPV TPPWDQVAAM
360 370 380 390
PTARLHLYGK EEARVGRKMG HVNFTAATLD EAVAGATACA RLLRIPLD
Length:398
Mass (Da):41,463
Last modified:January 24, 2006 - v1
Checksum:i245ED46131BE00EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37891.1.
CP008785 Genomic DNA. Translation: AIP25373.1.
RefSeqiWP_009892765.1. NZ_CM000438.1.
YP_441224.1. NC_007651.1.

Genome annotation databases

EnsemblBacteriaiABC37891; ABC37891; BTH_I0668.
GeneIDi3848781.
KEGGibte:BTH_I0668.
PATRICi19304101. VBIBurTha36512_3367.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37891.1.
CP008785 Genomic DNA. Translation: AIP25373.1.
RefSeqiWP_009892765.1. NZ_CM000438.1.
YP_441224.1. NC_007651.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IZOX-ray1.85A1-398[»]
ProteinModelPortaliQ2T0S5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi271848.BTH_I0668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC37891; ABC37891; BTH_I0668.
GeneIDi3848781.
KEGGibte:BTH_I0668.
PATRICi19304101. VBIBurTha36512_3367.

Phylogenomic databases

eggNOGiCOG0026.
HOGENOMiHOG000034029.
KOiK01589.
OrthoDBiEOG6QZMX7.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.
BioCyciBTHA271848:GJMY-668-MONOMER.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
    Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
    BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E264Imported and E264 / ATCC 700388 / DSM 13276 / CIP 106301Imported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: E264.
  3. "Crystal structure of kinase Phosphoribosylaminoimidazole carboxylase, ATPase subunit from Burkholderia thailandensis."
    SSGCID, Abendroth J., Lorimer D., Edwards T.E.
    Submitted (JAN-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: E264Imported.

Entry informationi

Entry nameiQ2T0S5_BURTA
AccessioniPrimary (citable) accession number: Q2T0S5
Entry historyi
Integrated into UniProtKB/TrEMBL: January 24, 2006
Last sequence update: January 24, 2006
Last modified: February 4, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.