Reviewed, UniProtKB/Swiss-Prot Q2T0L2 (MURB_BURTA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BTH_I0731

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000332450
Regions
	Domain	26 – 197	172	FAD-binding PCMH-type
Sites
	Active site	173	1	By similarity
	Active site	249	1	Proton donor By similarity
	Active site	345	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T0L2-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 1B349360A0749FDC	FASTA	349	37,811
	10 20 30 40 50 60 MPMSRPDSSV QLLSDYSLRA HNTFGFDVRA RVAARIGSPE QFASLARDPR VAGLDALVLG 70 80 90 100 110 120 GGSNVVFTRD FDGLVLLDEI RGRALAREDD DAWYVEAGGG ENWHAFVEWT LAEGMPGLEN 130 140 150 160 170 180 LALIPGTVGA APIQNIGAYG IEMKERFASL RAVELATGEI VEFDAARCAF GYRDSFFKRE 190 200 210 220 230 240 GRGRFAIVSV TFRLPKVWAP RLGYADVARE LAARGIDASR ASARDVFDAV VAIRRAKLPD 250 260 270 280 290 300 PLELGNAGSF FKNPVIDAHA YAALRAREPD VASYPQPDGR MKLAAGWLID RCGWKGRALG 310 320 330 340 AAAVHDRQAL VLVNRGGASG ADVLALARAI QRDVLERFGV ELEMEPVCL

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC39524.1. Different initiation.
RefSeq	YP_441287.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2T0L2.
Genome annotation databases
GeneID	3848372.
GenomeReviews	Gene locus BTH_I0731 in contig CP000086_GR.
KEGG	bte:BTH_I0731.
TIGR	BTH_I0731.
Phylogenomic databases
HOGENOM	Q2T0L2.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I0731-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BURTA

Accession

Primary (citable) accession number: Q2T0L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	September 1, 2009
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)