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Q2T0H6

- DUT_BURTA

UniProt

Q2T0H6 - DUT_BURTA

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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateUniRule annotation
Binding sitei94 – 941Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBTHA271848:GJMY-767-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:BTH_I0767
OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000001930: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_1000015457Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi271848.BTH_I0767.

Structurei

Secondary structure

1
148
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 96
Helixi10 – 145
Beta strandi27 – 304
Beta strandi37 – 393
Beta strandi44 – 5411
Beta strandi60 – 667
Helixi68 – 747
Beta strandi76 – 783
Beta strandi81 – 855
Beta strandi91 – 999
Beta strandi101 – 1033
Beta strandi105 – 1073
Beta strandi112 – 1209

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LHRX-ray1.50A1-148[»]
ProteinModelPortaliQ2T0H6.
SMRiQ2T0H6. Positions 2-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 693Substrate bindingUniRule annotation
Regioni84 – 863Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028968.
KOiK01520.
OMAiSIYIGDP.
OrthoDBiEOG689HXK.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2T0H6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLDLKILDA RMRDYLPKYA TTGSAGLDLR ACLDAPVTLK PGDTALVPTG
60 70 80 90 100
LAIHLADPGY AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GELMISTWNR
110 120 130 140
GQTEFVLNPF ERLAQLVIVP VVQATFNIVG DFAQSDRGAG GFGSTGRH
Length:148
Mass (Da):15,783
Last modified:January 24, 2006 - v1
Checksum:iDEF793839D83E4A4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000086 Genomic DNA. Translation: ABC36324.1.
RefSeqiYP_441323.1. NC_007651.1.

Genome annotation databases

EnsemblBacteriaiABC36324; ABC36324; BTH_I0767.
GeneIDi3846971.
KEGGibte:BTH_I0767.
PATRICi19304336. VBIBurTha36512_3484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000086 Genomic DNA. Translation: ABC36324.1 .
RefSeqi YP_441323.1. NC_007651.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4LHR X-ray 1.50 A 1-148 [» ]
ProteinModelPortali Q2T0H6.
SMRi Q2T0H6. Positions 2-132.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 271848.BTH_I0767.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC36324 ; ABC36324 ; BTH_I0767 .
GeneIDi 3846971.
KEGGi bte:BTH_I0767.
PATRICi 19304336. VBIBurTha36512_3484.

Phylogenomic databases

eggNOGi COG0756.
HOGENOMi HOG000028968.
KOi K01520.
OMAi SIYIGDP.
OrthoDBi EOG689HXK.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
BioCyci BTHA271848:GJMY-767-MONOMER.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
HAMAPi MF_00116. dUTPase_bact.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
    Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
    BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Entry informationi

Entry nameiDUT_BURTA
AccessioniPrimary (citable) accession number: Q2T0H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3