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Q2T0H3

- SYI_BURTA

UniProt

Q2T0H3 - SYI_BURTA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei581 – 5811Aminoacyl-adenylateUniRule annotation
    Binding sitei625 – 6251ATPUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation
    Metal bindingi931 – 9311ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBTHA271848:GJMY-770-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BTH_I0770
    OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
    Taxonomic identifieri271848 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000001930: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 945945Isoleucine--tRNA ligasePRO_1000022049Add
    BLAST

    Proteomic databases

    PRIDEiQ2T0H3.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi271848.BTH_I0770.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2T0H3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7611"HIGH" regionAdd
    BLAST
    Motifi622 – 6265"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2T0H3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKDW EARGVYEKIR    50
    ATSQGRPKFI LHDGPPYANG DIHLGHAVNK ILKDMVVKSR NMAGFDAPYV 100
    PGWDCHGMPI EIQIEKQFGK SLPAAEVMAK ARAYATEQIE KQKAGFKRLG 150
    VLGEWGNPYK TMNFQNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL 200
    AEAEVEYKDR TDPTIDVLFA FAEPEKTAHA FGLAALPRAE GGIVIWTTTP 250
    WTIPANQALN LHPEIVYALV DTERGLLVMA EERVEACMKD FGLTGRVVAT 300
    TRGDKLANLR FHHPLAAAHP GYKRTSPVYL GDYVTTDTGT GIVHSSPAYG 350
    VEDFTSCKAH GMTDSDIINP VMGDGRYIES LPLFGGLTIW DANPKIVDAL 400
    KAAGSLLRNE HYSHSYMHCW RHKTPIIYRA TSQWFAGMDT QPADGGKTLR 450
    ETALDAVDAT AFYPSWGKQR LHAMIANRPD WTLSRQRQWG VPMAFFVHKE 500
    TGELHPRTLE LLEEVAKRVE QQGIEAWQTL DARELIGDDA NLYEKNRDTL 550
    DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASML 600
    DGRAPYKGLL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA 650
    STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD YAKDALPAEQ 700
    WLEIDRYAVA FSAQLQAELL AHYEKYEFHP VVAKLQTFCS EDLGGFYLDV 750
    LKDRLYTSAP ASKARRSAQT ALYHVTQGLL RVLAPFLSFT AEEAWRVFLP 800
    QSETIFTETY YAYPEIANAD ALVAKWTLLR DVRGNVTKAL EEARAANRIG 850
    SSLQAQVEVR ASGARYDALA SLGDDLKFVL ITSAATVVKV DAQSDESVEV 900
    AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE TRSAA 945
    Length:945
    Mass (Da):105,830
    Last modified:January 24, 2006 - v1
    Checksum:i8FFF33FDD07F4558
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000086 Genomic DNA. Translation: ABC38192.1.
    RefSeqiYP_441326.1. NC_007651.1.

    Genome annotation databases

    EnsemblBacteriaiABC38192; ABC38192; BTH_I0770.
    GeneIDi3849667.
    KEGGibte:BTH_I0770.
    PATRICi19304342. VBIBurTha36512_3487.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000086 Genomic DNA. Translation: ABC38192.1 .
    RefSeqi YP_441326.1. NC_007651.1.

    3D structure databases

    ProteinModelPortali Q2T0H3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 271848.BTH_I0770.

    Proteomic databases

    PRIDEi Q2T0H3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC38192 ; ABC38192 ; BTH_I0770 .
    GeneIDi 3849667.
    KEGGi bte:BTH_I0770.
    PATRICi 19304342. VBIBurTha36512_3487.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BTHA271848:GJMY-770-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
      Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
      BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

    Entry informationi

    Entry nameiSYI_BURTA
    AccessioniPrimary (citable) accession number: Q2T0H3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3