Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei581Aminoacyl-adenylateUniRule annotation1
Binding sitei625ATPUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi928ZincUniRule annotation1
Metal bindingi931ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BTH_I0770
OrganismiBurkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000001930 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220491 – 945Isoleucine--tRNA ligaseAdd BLAST945

Proteomic databases

PRIDEiQ2T0H3

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2T0H3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi66 – 76"HIGH" regionAdd BLAST11
Motifi622 – 626"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q2T0H3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKDW EARGVYEKIR
60 70 80 90 100
ATSQGRPKFI LHDGPPYANG DIHLGHAVNK ILKDMVVKSR NMAGFDAPYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK SLPAAEVMAK ARAYATEQIE KQKAGFKRLG
160 170 180 190 200
VLGEWGNPYK TMNFQNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDR TDPTIDVLFA FAEPEKTAHA FGLAALPRAE GGIVIWTTTP
260 270 280 290 300
WTIPANQALN LHPEIVYALV DTERGLLVMA EERVEACMKD FGLTGRVVAT
310 320 330 340 350
TRGDKLANLR FHHPLAAAHP GYKRTSPVYL GDYVTTDTGT GIVHSSPAYG
360 370 380 390 400
VEDFTSCKAH GMTDSDIINP VMGDGRYIES LPLFGGLTIW DANPKIVDAL
410 420 430 440 450
KAAGSLLRNE HYSHSYMHCW RHKTPIIYRA TSQWFAGMDT QPADGGKTLR
460 470 480 490 500
ETALDAVDAT AFYPSWGKQR LHAMIANRPD WTLSRQRQWG VPMAFFVHKE
510 520 530 540 550
TGELHPRTLE LLEEVAKRVE QQGIEAWQTL DARELIGDDA NLYEKNRDTL
560 570 580 590 600
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASML
610 620 630 640 650
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA
660 670 680 690 700
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD YAKDALPAEQ
710 720 730 740 750
WLEIDRYAVA FSAQLQAELL AHYEKYEFHP VVAKLQTFCS EDLGGFYLDV
760 770 780 790 800
LKDRLYTSAP ASKARRSAQT ALYHVTQGLL RVLAPFLSFT AEEAWRVFLP
810 820 830 840 850
QSETIFTETY YAYPEIANAD ALVAKWTLLR DVRGNVTKAL EEARAANRIG
860 870 880 890 900
SSLQAQVEVR ASGARYDALA SLGDDLKFVL ITSAATVVKV DAQSDESVEV
910 920 930 940
AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE TRSAA
Length:945
Mass (Da):105,830
Last modified:January 24, 2006 - v1
Checksum:i8FFF33FDD07F4558
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA Translation: ABC38192.1
RefSeqiWP_009908562.1, NC_007651.1

Genome annotation databases

EnsemblBacteriaiABC38192; ABC38192; BTH_I0770
KEGGibte:BTH_I0770

Similar proteinsi

Entry informationi

Entry nameiSYI_BURTA
AccessioniPrimary (citable) accession number: Q2T0H3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health