Reviewed, UniProtKB/Swiss-Prot Q2T087 (SYM_BURTA)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BTH_I0856

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331797
Regions
	Domain	612 – 718	107	tRNA-binding
	Motif	27 – 37	11	"HIGH" region HAMAP MF_00098
	Motif	348 – 352	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	158	1	Zinc By similarity
	Metal binding	161	1	Zinc By similarity
	Metal binding	171	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
	Binding site	351	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2T087-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 4DC90AD0ECA7ED19	FASTA	718	79,413
	10 20 30 40 50 60 MSASDLHPVQ AGAPQGRRQI LVTSALPYAN GQIHIGHLVE YIQTDIWART MRMHGHEIYY 70 80 90 100 110 120 IGADDTHGTP VMLRAEQEGV SPKQLIERVW REHKRDFDSF GVSFDNFYTT DSDENRVLSE 130 140 150 160 170 180 KIYLALKEAG FIAEREIEQA YDPVKQMFLP DRFIKGECPK CHAKDQYGDS CEVCGTTYQP 190 200 210 220 230 240 TDLVNPYSVV SGATPVRKTS THHFFRLSDP RCEAFLREWV SGLAQPEATN KMREWLGDAG 250 260 270 280 290 300 EAKLADWDIS RDAPYFGFEI PGAPGKYFYV WLDAPVGYYA SFKNLCERRG LDFDAWISKD 310 320 330 340 350 360 STTEQYHFIG KDILYFHTLF WPAMLEFSGH RTPTNVFAHG FLTVDGAKMS KSRGTFITAQ 370 380 390 400 410 420 SYIDAGLNPE WLRYYFAAKL NATMEDIDLN LEDFQARVNS DLVGKYVNIA SRAAGFLIKR 430 440 450 460 470 480 FDGRVQASAT NHPLLVTLRD AIPQIAAHYE AREYGRALRQ TMELADAVNG YVDTAKPWEL 490 500 510 520 530 540 AKDPANAVAL HETCTVSLEA FRLLSLALKP VLPRVAEGVE AFLGIAPLTW ADANKPLSSE 550 560 570 580 590 600 QPIRAYQHLM TRVDPKQIDA LLAANRSSLQ GAAAADAAGA TNGNGAKAAK SAKAANAASA 610 620 630 640 650 660 DDEASPFISI DDFAKIDLRI AKIVACQAVE GSDKLLQLTL DVGEEKTRNV FSGIKSAYQP 670 680 690 700 710 EQLVGKLTVM VANLAPRKMK FGLSEGMVLA ASAADEKAEP GLYILEPHSG AKPGMRVK

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC38961.1. Different initiation.
RefSeq	YP_441412.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3848452.
GenomeReviews	Gene locus BTH_I0856 in contig CP000086_GR.
KEGG	bte:BTH_I0856.
TIGR	BTH_I0856.
Phylogenomic databases
HOGENOM	Q2T087.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I0856-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BURTA

Accession

Primary (citable) accession number: Q2T087

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	June 16, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)