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Protein

Glutamate 5-kinase

Gene

proB

Organism
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATPUniRule annotation1
Binding sitei54SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei153Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 174ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:BTH_I1143
OrganismiBurkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000001930 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002529731 – 372Glutamate 5-kinaseAdd BLAST372

Proteomic databases

PRIDEiQ2SZF9

Structurei

Secondary structure

1372
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Beta strandi9 – 15Combined sources7
Helixi17 – 20Combined sources4
Helixi29 – 44Combined sources16
Beta strandi48 – 53Combined sources6
Helixi56 – 64Combined sources9
Helixi73 – 96Combined sources24
Turni97 – 99Combined sources3
Beta strandi102 – 107Combined sources6
Helixi109 – 113Combined sources5
Helixi115 – 130Combined sources16
Beta strandi134 – 139Combined sources6
Turni141 – 143Combined sources3
Turni146 – 148Combined sources3
Helixi154 – 164Combined sources11
Beta strandi167 – 175Combined sources9
Beta strandi193 – 196Combined sources4
Helixi219 – 229Combined sources11
Turni230 – 232Combined sources3
Beta strandi234 – 239Combined sources6
Helixi245 – 250Combined sources6
Beta strandi256 – 260Combined sources5
Helixi265 – 276Combined sources12
Beta strandi277 – 285Combined sources9
Helixi287 – 294Combined sources8
Helixi302 – 304Combined sources3
Beta strandi305 – 310Combined sources6
Beta strandi317 – 321Combined sources5
Beta strandi327 – 332Combined sources6
Helixi337 – 343Combined sources7
Helixi348 – 350Combined sources3
Helixi351 – 355Combined sources5
Helixi366 – 368Combined sources3
Beta strandi369 – 371Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q1TX-ray2.15A/B/C/D1-372[»]
ProteinModelPortaliQ2SZF9
SMRiQ2SZF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 358PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246368
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q2SZF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSIIADSKR LVVKVGSSLV TNDGRGLDHD AIGRWAAQIA ALRNEGKEVV
60 70 80 90 100
LVSSGAIAEG MQRLGWSRRP REIDELQAAA AVGQMGLAQV YESRFAEHGI
110 120 130 140 150
RTAQILLTHA DLADRERYLN ARSTLLTLLR LGVVPIINEN DTVVTDEIKF
160 170 180 190 200
GDNDTLGALV ANLIEGDALI ILTDQQGLFT ADPRKDPGAT LVAEASAGAP
210 220 230 240 250
ELEAMAGGAG SSIGRGGMLT KILAAKRAAH SGANTVIASG RERDVLLRLA
260 270 280 290 300
SGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV DKLTAGGKSL
310 320 330 340 350
LPIGVVAVQG VFARGEVIAC VNDAGREVAR GITNYSSAEA KLIQRKPSGE
360 370
IEAVLGYMLE PELIHRDNLV LV
Length:372
Mass (Da):39,330
Last modified:January 24, 2006 - v1
Checksum:i777EE3DFCF77C4A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA Translation: ABC38938.1
RefSeqiWP_009888926.1, NZ_CM000438.1

Genome annotation databases

EnsemblBacteriaiABC38938; ABC38938; BTH_I1143
KEGGibte:BTH_I1143

Similar proteinsi

Entry informationi

Entry nameiPROB_BURTA
AccessioniPrimary (citable) accession number: Q2SZF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: January 24, 2006
Last modified: May 23, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

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