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Q2SZF9

- PROB_BURTA

UniProt

Q2SZF9 - PROB_BURTA

Protein

Glutamate 5-kinase

Gene

proB

Organism
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATPUniRule annotation
    Binding sitei54 – 541SubstrateUniRule annotation
    Binding sitei141 – 1411SubstrateUniRule annotation
    Binding sitei153 – 1531Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi173 – 1742ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBTHA271848:GJMY-1143-MONOMER.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    Ordered Locus Names:BTH_I1143
    OrganismiBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
    Taxonomic identifieri271848 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000001930: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Glutamate 5-kinasePRO_0000252973Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi271848.BTH_I1143.

    Structurei

    Secondary structure

    1
    372
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 74
    Beta strandi9 – 157
    Helixi17 – 204
    Helixi29 – 4416
    Beta strandi48 – 536
    Helixi56 – 649
    Helixi73 – 9624
    Turni97 – 993
    Beta strandi102 – 1076
    Helixi109 – 1135
    Helixi115 – 13016
    Beta strandi134 – 1396
    Turni141 – 1433
    Turni146 – 1483
    Helixi154 – 16411
    Beta strandi167 – 1759
    Beta strandi193 – 1964
    Helixi219 – 22911
    Turni230 – 2323
    Beta strandi234 – 2396
    Helixi245 – 2506
    Beta strandi256 – 2605
    Helixi265 – 27612
    Beta strandi277 – 2859
    Helixi287 – 2948
    Helixi302 – 3043
    Beta strandi305 – 3106
    Beta strandi317 – 3215
    Beta strandi327 – 3326
    Helixi337 – 3437
    Helixi348 – 3503
    Helixi351 – 3555
    Helixi366 – 3683
    Beta strandi369 – 3713

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4Q1TX-ray2.15A/B/C/D1-372[»]
    ProteinModelPortaliQ2SZF9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini280 – 35879PUAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation
    Contains 1 PUA domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0263.
    HOGENOMiHOG000246368.
    KOiK00931.
    OMAiMRMIAGH.
    OrthoDBiEOG6PGK7G.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2SZF9-1 [UniParc]FASTAAdd to Basket

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    MRSIIADSKR LVVKVGSSLV TNDGRGLDHD AIGRWAAQIA ALRNEGKEVV    50
    LVSSGAIAEG MQRLGWSRRP REIDELQAAA AVGQMGLAQV YESRFAEHGI 100
    RTAQILLTHA DLADRERYLN ARSTLLTLLR LGVVPIINEN DTVVTDEIKF 150
    GDNDTLGALV ANLIEGDALI ILTDQQGLFT ADPRKDPGAT LVAEASAGAP 200
    ELEAMAGGAG SSIGRGGMLT KILAAKRAAH SGANTVIASG RERDVLLRLA 250
    SGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV DKLTAGGKSL 300
    LPIGVVAVQG VFARGEVIAC VNDAGREVAR GITNYSSAEA KLIQRKPSGE 350
    IEAVLGYMLE PELIHRDNLV LV 372
    Length:372
    Mass (Da):39,330
    Last modified:January 24, 2006 - v1
    Checksum:i777EE3DFCF77C4A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000086 Genomic DNA. Translation: ABC38938.1.
    RefSeqiYP_441690.1. NC_007651.1.

    Genome annotation databases

    EnsemblBacteriaiABC38938; ABC38938; BTH_I1143.
    GeneIDi3848545.
    KEGGibte:BTH_I1143.
    PATRICi19305178. VBIBurTha36512_3898.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000086 Genomic DNA. Translation: ABC38938.1 .
    RefSeqi YP_441690.1. NC_007651.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4Q1T X-ray 2.15 A/B/C/D 1-372 [» ]
    ProteinModelPortali Q2SZF9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 271848.BTH_I1143.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC38938 ; ABC38938 ; BTH_I1143 .
    GeneIDi 3848545.
    KEGGi bte:BTH_I1143.
    PATRICi 19305178. VBIBurTha36512_3898.

    Phylogenomic databases

    eggNOGi COG0263.
    HOGENOMi HOG000246368.
    KOi K00931.
    OMAi MRMIAGH.
    OrthoDBi EOG6PGK7G.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci BTHA271848:GJMY-1143-MONOMER.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
      Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
      BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

    Entry informationi

    Entry nameiPROB_BURTA
    AccessioniPrimary (citable) accession number: Q2SZF9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3