Reviewed, UniProtKB/Swiss-Prot Q2SZF9 (PROB_BURTA)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BTH_I1143

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glutamate 5-kinase HAMAP MF_00456		PRO_0000252973
Regions
	Domain	280 – 358	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q2SZF9-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 777EE3DFCF77C4A6	FASTA	372	39,330
	10 20 30 40 50 60 MRSIIADSKR LVVKVGSSLV TNDGRGLDHD AIGRWAAQIA ALRNEGKEVV LVSSGAIAEG 70 80 90 100 110 120 MQRLGWSRRP REIDELQAAA AVGQMGLAQV YESRFAEHGI RTAQILLTHA DLADRERYLN 130 140 150 160 170 180 ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDALI ILTDQQGLFT 190 200 210 220 230 240 ADPRKDPGAT LVAEASAGAP ELEAMAGGAG SSIGRGGMLT KILAAKRAAH SGANTVIASG 250 260 270 280 290 300 RERDVLLRLA SGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV DKLTAGGKSL 310 320 330 340 350 360 LPIGVVAVQG VFARGEVIAC VNDAGREVAR GITNYSSAEA KLIQRKPSGE IEAVLGYMLE 370 PELIHRDNLV LV

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC38938.1.
RefSeq	YP_441690.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SZF9.
Genome annotation databases
GeneID	3848545.
GenomeReviews	Gene locus BTH_I1143 in contig CP000086_GR.
KEGG	bte:BTH_I1143.
TIGR	BTH_I1143.
Phylogenomic databases
HOGENOM	Q2SZF9.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1143-MON.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BURTA

Accession

Primary (citable) accession number: Q2SZF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	January 24, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)