Reviewed, UniProtKB/Swiss-Prot Q2SYZ9 (HEMH_BURTA)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BTH_I1303

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Ferrochelatase HAMAP MF_00323		PRO_1000059476
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SYZ9-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: B04F91B3988547DF	FASTA	355	39,370
	10 20 30 40 50 60 MRFDLEPPSH ASAAHRVAVL LVNLGTPDEP TPRAVRRYLA QFLSDPRVVE IPQLVWQVIL 70 80 90 100 110 120 RTLILPLRGR ASAKKYAAVW LPEGSPLRVY TERQVESLKP LFAANGYRVI VDYAMRYGTP 130 140 150 160 170 180 SIADVLAQLK RAGAERVLLL PMYPQYSAST TATAFDAAFA ALGRMRNQPE VRTVRHYADH 190 200 210 220 230 240 PAYIHALAEQ VRQYWAAHGR PAFDSGDKLV LSFHGVPKRT LDLGDPYHDQ CQQTAALLMS 250 260 270 280 290 300 ALGLTTFECR VTFQSRFGKA EWLQPYTAPT LKELGAAGVR RADVFCPGFT ADCLETIEEI 310 320 330 340 350 GMEVRDEFLH GGGKEFHRIP CLNASHAWIA ALGEIAAENL QGWPVRVATA PEAVT

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC36533.1.
RefSeq	YP_441850.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3847181.
GenomeReviews	Gene locus BTH_I1303 in contig CP000086_GR.
KEGG	bte:BTH_I1303.
TIGR	BTH_I1303.
Phylogenomic databases
HOGENOM	Q2SYZ9.
OMA	Q2SYZ9. TIEEIGM.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1303-MON.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURTA

Accession

Primary (citable) accession number: Q2SYZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)