ID   MIAA_BURTA              Reviewed;         324 AA.
AC   Q2SYY3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=tRNA Delta(2)-isopentenylpyrophosphate transferase;
DE            Short=IPP transferase;
DE            EC=2.5.1.8;
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
DE            Short=IPTase;
DE            Short=IPPT;
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
DE            Short=DMAPP:tRNA dimethylallyltransferase;
DE            Short=DMATase;
GN   Name=miaA; OrderedLocusNames=BTH_I1319;
OS   Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 /
OS   CIP 106301).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R.,
RA   Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D.,
RA   Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J.,
RA   Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D.,
RA   Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P.,
RA   Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F.,
RA   Roberts K., Hatch B., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate +
CC       tRNA containing 6-isopentenyladenosine.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
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DR   EMBL; CP000086; ABC38550.1; -; Genomic_DNA.
DR   RefSeq; YP_441866.1; -.
DR   GeneID; 3848084; -.
DR   GenomeReviews; CP000086_GR; BTH_I1319.
DR   KEGG; bte:BTH_I1319; -.
DR   TIGR; BTH_I1319; -.
DR   HOGENOM; Q2SYY3; -.
DR   OMA; Q2SYY3; VNADSMQ.
DR   BioCyc; BTHA271848:BTH_I1319-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004811; F:tRNA isopentenyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:HAMAP.
DR   HAMAP; MF_00185; -; 1.
DR   InterPro; IPR002627; IPPT.
DR   InterPro; IPR018022; tRNA_delta_PyrP_Trfase.
DR   PANTHER; PTHR11088; IPPT; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   ProDom; PD004674; IPPT; 1.
DR   ProDom; PD005388; IPPtrans_like; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Magnesium; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; tRNA processing.
FT   CHAIN         1    324       tRNA Delta(2)-isopentenylpyrophosphate
FT                                transferase.
FT                                /FTId=PRO_1000020579.
FT   NP_BIND      17     24       ATP (Potential).
FT   REGION       19     24       Substrate binding (By similarity).
FT   REGION       42     45       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      166    170       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      251    256       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        108    108       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        130    130       Interaction with substrate tRNA (By
FT                                similarity).
SQ   SEQUENCE   324 AA;  34998 MW;  BF8FDD8A9BD29A64 CRC64;
     MSESNAASVR TVACLLGPTA SGKTAAALAL AARRPIEIVS VDSALVYRGM DIGTAKPTRE
     ERAAVPHHLI DIVDPADAYS AAGFRADALR VVAEIAARGR TPLLAGGTML YYKALTQGLN
     DLPAADPDVR ATLDAEAARD GWPALHARLA SVDPATAARL APNDAQRIQR ALEVYLLTGR
     PMSALLAAPP RNDDAAAGLR FVPVALEPSD RAVLHARIAA RFDAMLEAGF IDEVERLRRR
     DDLHLGLPSM RCVGYRQAWE YLEGVTDYRM MRDKGIFATR QLCKRQLTWL RAMPARIVVD
     CCAQDATARA VDALERVLDG RTPA
//