Reviewed, UniProtKB/Swiss-Prot Q2SYY3 (MIAA_BURTA)

Names and origin

Protein names

Recommended name:
    tRNA Delta(2)-isopentenylpyrophosphate transferase
      Short name=IPP transferase
    EC=2.5.1.8
Alternative name(s):
    Isopentenyl-diphosphate:tRNA isopentenyltransferase
      Short name=IPTase
      Short name=IPPT
    Dimethylallyl diphosphate:tRNA dimethylallyltransferase
      Short name=DMAPP:tRNA dimethylallyltransferase
      Short name=DMATase

Gene names

Name:	miaA
Ordered Locus Names:	BTH_I1319

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity.
Catalytic activity	Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW tRNA isopentenyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	tRNA Delta(2)-isopentenylpyrophosphate transferase		PRO_1000020579
Regions
	Nucleotide binding	17 – 24	8	ATP Potential
	Region	19 – 24	6	Substrate binding By similarity
	Region	42 – 45	4	Interaction with substrate tRNA By similarity
	Region	166 – 170	5	Interaction with substrate tRNA By similarity
	Region	251 – 256	6	Interaction with substrate tRNA By similarity
Sites
	Site	108	1	Interaction with substrate tRNA By similarity
	Site	130	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SYY3-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: BF8FDD8A9BD29A64	FASTA	324	34,998
	10 20 30 40 50 60 MSESNAASVR TVACLLGPTA SGKTAAALAL AARRPIEIVS VDSALVYRGM DIGTAKPTRE 70 80 90 100 110 120 ERAAVPHHLI DIVDPADAYS AAGFRADALR VVAEIAARGR TPLLAGGTML YYKALTQGLN 130 140 150 160 170 180 DLPAADPDVR ATLDAEAARD GWPALHARLA SVDPATAARL APNDAQRIQR ALEVYLLTGR 190 200 210 220 230 240 PMSALLAAPP RNDDAAAGLR FVPVALEPSD RAVLHARIAA RFDAMLEAGF IDEVERLRRR 250 260 270 280 290 300 DDLHLGLPSM RCVGYRQAWE YLEGVTDYRM MRDKGIFATR QLCKRQLTWL RAMPARIVVD 310 320 CCAQDATARA VDALERVLDG RTPA

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC38550.1.
RefSeq	YP_441866.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3848084.
GenomeReviews	Gene locus BTH_I1319 in contig CP000086_GR.
KEGG	bte:BTH_I1319.
TIGR	BTH_I1319.
Phylogenomic databases
HOGENOM	Q2SYY3.
OMA	Q2SYY3. VNADSMQ.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1319-MON.
Family and domain databases
HAMAP	MF_00185. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
ProDom	PD004674. IPPT. 1 hit. PD005388. IPPtrans_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00174. miaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_BURTA

Accession

Primary (citable) accession number: Q2SYY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)