Reviewed, UniProtKB/Swiss-Prot Q2SYE0 (TRMB_BURTA)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	BTH_I1513

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000288130
Regions
	Region	241 – 244	4	Substrate binding Potential
Sites
	Binding site	95	1	S-adenosyl-L-methionine By similarity
	Binding site	120	1	S-adenosyl-L-methionine By similarity
	Binding site	147	1	S-adenosyl-L-methionine By similarity
	Binding site	170	1	S-adenosyl-L-methionine By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	206	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2SYE0-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 346228F881714D97	FASTA	265	29,365
	10 20 30 40 50 60 MNHDDPNASG VPHDDANDAA PASASDAARA TGHADDESSP LHLRRIRSFV TRAGRVSTGQ 70 80 90 100 110 120 RRAIDELGPR FVVPYDNAQP DWDTVFGRRA PRVLEIGFGM GASTAEIAAH RPGDDFIGVE 130 140 150 160 170 180 VHEPGVGALL KLIGEQQLSN IRIIQHDAVE VLEHMIAPDS LDGAHIFFPD PWHKARHHKR 190 200 210 220 230 240 RLIQPPFVAQ LAARLKPGAY LHCATDWQNY AEQMLEVLGA DPSLENTAQD YAPRPDYRPV 250 260 TKFERRGLRL GHGVWDLVFR KKRAG

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC36287.1.
RefSeq	YP_442059.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3846934.
GenomeReviews	Gene locus BTH_I1513 in contig CP000086_GR.
KEGG	bte:BTH_I1513.
TIGR	BTH_I1513.
Phylogenomic databases
HOGENOM	Q2SYE0.
OMA	Q2SYE0. TKFENRG.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1513-MON.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_BURTA

Accession

Primary (citable) accession number: Q2SYE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)