Reviewed, UniProtKB/Swiss-Prot Q2SYA5 (GLK_BURTA)

Names and origin

Protein names

Recommended name:
    Bifunctional protein glk
Including the following 2 domains:
    1- Recommended name:
            Glucokinase
              EC=2.7.1.2
        Alternative name(s):
            Glucose kinase
    2- Recommended name:
            Putative HTH-type transcriptional regulator

Gene names

Name:	glk
Ordered Locus Names:	BTH_I1550

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate. HAMAP MF_00524
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the bacterial glucokinase family. Contains 1 HTH rpiR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Glycolysis Transcription Transcription regulation
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glucokinase activity Inferred from electronic annotation. Source: HAMAP sugar binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Bifunctional protein glk HAMAP MF_00524		PRO_0000268801
Regions
	Transmembrane	576 – 596	21	Potential
	Domain	341 – 417	77	HTH rpiR-type
	Nucleotide binding	23 – 28	6	ATP Potential
	DNA binding	377 – 396	20	H-T-H motif By similarity
	Region	1 – 340	340	Glucokinase HAMAP MF_00524
	Region	341 – 641	301	Putative HTH-type transcriptional regulator HAMAP MF_00524

Sequences

	Sequence		Length	Mass (Da)
	Q2SYA5-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 06726A47707F8B7F	FASTA	641	68,475
	10 20 30 40 50 60 MSTGAQTKAA EASQHADGPR LLADVGGTNA RFALETGPGE ITQIRVYPGA EYPTLTDAIR 70 80 90 100 110 120 RYLKDVKIGR VNHAAIAIAN PVDGDQVRMT NHNWSFSIEA TRRALGFDTL LVVNDFTALA 130 140 150 160 170 180 MALPGLTDAQ RVQIGAGARR QNSVIGLMGP GTGLGVSGLI PADDRWIALG SEGGHATFAP 190 200 210 220 230 240 MDEREDLVLQ YARRKYPHVS FERVCAGPGM EIIYRALAAR DKKRIAANVV TADIVERAHA 250 260 270 280 290 300 GDALALEAVE CFCGILGTFA GNLAVTLGAL GGIYIGGGVV PKLGELFMRS PFRARFEAKG 310 320 330 340 350 360 RFEAYLANIP TYLITAEYPA FLGVSAILAE QLSNRTGGAS SAVFERIRQM RDALTPAERR 370 380 390 400 410 420 VADLALNHPR SIINDPIVDI ARKADVSQPT VIRFCRSLGC QGLSDFKLKL ATGLTGTIPM 430 440 450 460 470 480 SHSQVHLGDT ATDFGAKVLD NTVSAILQLR EHLNFEHVEQ AIDILNNARR IEFYGLGNSN 490 500 510 520 530 540 IVAQDAHYKF FRFGIPTIAY GDLYMQAASA ALLGKGDVIV AVSKSGRAPE LLRVLDVAMQ 550 560 570 580 590 600 AGAKVIAITS SNTPLAKRAT VALETDHIEM RESQLSMISR ILHLVMIDIL AVGVAIRRAS 610 620 630 640 PNAELAEAMA RAKARAGASA GDEAADVLDW LSHGAAPAAK E

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC37839.1.
RefSeq	YP_442094.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3850093.
GenomeReviews	Gene locus BTH_I1550 in contig CP000086_GR.
KEGG	bte:BTH_I1550.
TIGR	BTH_I1550.
Phylogenomic databases
HOGENOM	Q2SYA5.
OMA	Q2SYA5. HYLQNIP.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1550-MON.
Family and domain databases
HAMAP	MF_00524. Fused. [Tree]
InterPro	IPR003836. Glucokinase. IPR000843. HTH_LacI. IPR000281. HTH_RpiR. IPR001347. SIS. [Graphical view]
Pfam	PF02685. Glucokinase. 1 hit. PF01418. HTH_6. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00749. glk. 1 hit.
PROSITE	PS00356. HTH_LACI_1. 1 hit. PS51071. HTH_RPIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLK_BURTA

Accession

Primary (citable) accession number: Q2SYA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)