Q2SY74 (RNPH_BURTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BTH_I1584

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Ribonuclease PH HAMAP MF_00564		PRO_1000024791

Sequences

	Sequence		Length	Mass (Da)
	Q2SY74 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 0D77F5A3603C260A	FASTA	243	26,034
	10 20 30 40 50 60 MTNSSPRPSG RRADQLRDVR ITRHYTKHAE GSVLVEFGET KVICTASVVE RVPEFLRERG 70 80 90 100 110 120 QGWLTAEYGM LPRATHTRSD REAARGKQTG RTQEIQRLIG RALRAVFDLN ALGPRTLHLD 130 140 150 160 170 180 CDVIQADGGT RTASITGAFV AAHDAVTKLV AAGKIARSPI TDYVAAISVG VFGGAPVLDL 190 200 210 220 230 240 DYDEDSACDT DMNVVMTGAG GFVEVQGTAE GVPFSRAEMN ALLDLAQAGI GELVRLQRAA LEA

References

[1]

"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies." Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D. BMC Genomics 6:174-174(2005) [PubMed: 16336651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000086 Genomic DNA. Translation: ABC37325.1.
RefSeq	YP_442125.1. NC_007651.1.
3D structure databases
ProteinModelPortal	Q2SY74.
SMR	Q2SY74. Positions 7-241.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SY74.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3848958.
GenomeReviews	Gene locus BTH_I1584 in contig CP000086_GR.
KEGG	bte:BTH_I1584.
PATRIC	19306130. VBIBurTha36512_4370.
TIGR	BTH_I1584.
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1584-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BURTA

Accession

Primary (citable) accession number: Q2SY74

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program