Q2SY55 (METE_BURTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BTH_I1606

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000071608
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SY55 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 80C579164EF2CE52	FASTA	764	84,429
	10 20 30 40 50 60 MTTAHILGFP RIGAQRELKF ALERYWRDGA SADAERALVD TGRALRAAHW QTQRDAGLDC 70 80 90 100 110 120 VTVGDFAWYD HVLTTLAHVG GLPRRFGFDA RALTLADYFA AARGNAAQPA MEMTKWFDTN 130 140 150 160 170 180 YHYLVPEYSP ATTFGPGVEW LFDEVREARA LGHRPKAALV GPLTLLWLGK ARDGLADRLE 190 200 210 220 230 240 LLPRLVPAYR ALLARLREAG VDWVQIDEPI FALDLPAAWR DAARPAYEAL VPGAPKLLVA 250 260 270 280 290 300 TYFDDVSEHA ALLKALPVAG LHVDLVRGDA QLDAFVADYP ADKVLSCGIV DGRNVWRNDL 310 320 330 340 350 360 DRSLARLAPV HETLGERLWI ATSCSLLHAP VDLAHEPKLD DELKAWLAFA VQKTREVAAL 370 380 390 400 410 420 RDALAKGRAA VAAEFDAAAV AAAARRTSAR IHNPLVKRRV AALTDADARR ASAYPARAAA 430 440 450 460 470 480 QRARFDLPPL PTTTIGSFPQ TPEIRRARAA FRQGVLDHLG YLEAMREQVR IAIDKQLSYG 490 500 510 520 530 540 LDVLVHGEAE RNDMVEYFGE LMWGFAITSN GWVQSYGSRC VKPPLVYGDV YLPEPMTVGW 550 560 570 580 590 600 ASYAQSLTTK PVKGMLTGPV TMLQWSFVRD DQPRATTALQ IALALRQETL DLEKAGIGMI 610 620 630 640 650 660 QVDEPALREG LPLKARDRAE YLDWAVRAFR IAASGVADDT QIHTHMCYSE FGDILPSIAA 670 680 690 700 710 720 LDADVISIET TRSNMELLDA FETFEYPNEI GPGVYDIHSP RVPDADEIER LILLALERIP 730 740 750 760 AQRLWVNPDC GLKTREWRQV DAALAAMVDA AKRVRQTVEQ AVPA

References

[1]

"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies." Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D. BMC Genomics 6:174-174(2005) [PubMed: 16336651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000086 Genomic DNA. Translation: ABC38605.1.
RefSeq	YP_442144.1. NC_007651.1.
3D structure databases
HSSP	HSSP built from PDB template 1U1J based on UniProtKB O50008.
ProteinModelPortal	Q2SY55.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SY55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3847877.
GenomeReviews	Gene locus BTH_I1606 in contig CP000086_GR.
KEGG	bte:BTH_I1606.
PATRIC	19306178. VBIBurTha36512_4391.
TIGR	BTH_I1606.
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	NIWANDF.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1606-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURTA

Accession

Primary (citable) accession number: Q2SY55

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program