Q2SXJ3 (HUTI_BURTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BTH_I1824

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Imidazolonepropionase HAMAP MF_00372		PRO_0000306454
Sites
	Metal binding	68	1	Zinc or iron By similarity
	Metal binding	70	1	Zinc or iron By similarity
	Metal binding	238	1	Zinc or iron By similarity
	Metal binding	313	1	Zinc or iron By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SXJ3 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: D3D5C541E99057CC	FASTA	407	43,715
	10 20 30 40 50 60 MKSTLWHNLK LCAHGDPNDT IADAAIAVNG DGTIAWTGRA SDVPAGYVHW PREDLRGAWV 70 80 90 100 110 120 TPGLVDCHTH LVYGGQRADE FAQRLAGVSY EEIARRGGGI VSTVRATRDA SEATLFEQAA 130 140 150 160 170 180 ARLRPLLAEG VTAIEIKSGY GLELASERRM LRVARQLGER FPVSVYTTFL GAHALPPEYA 190 200 210 220 230 240 GRADEYVDEV CERMLPALAD EGLVDAVDVF CERIGFTLAQ SERVFEAAAR RGLPVKMHAE 250 260 270 280 290 300 QLSGGGGAAL AARYRALSAD HLEYLDEAGV AAMRASGTTA VLLPGAYYFI RETKQPPIDL 310 320 330 340 350 360 LRRHGVPIAL ATDHNPGTSP LTSLLLTMNM GCTLFKLTVQ EALLGVTRHA AAALGAGDRH 370 380 390 400 GSLAPGRQAD FAVWSAATLA ELAYWFGRPL CERVVKGGVT VFARDAR

References

[1]

"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies." Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D. BMC Genomics 6:174-174(2005) [PubMed: 16336651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000086 Genomic DNA. Translation: ABC38786.1.
RefSeq	YP_442356.1. NC_007651.1.
3D structure databases
HSSP	HSSP built from PDB template 2GOK based on UniProtKB Q8U8Z6.
ProteinModelPortal	Q2SXJ3.
SMR	Q2SXJ3. Positions 3-401.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2SXJ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3849678.
GenomeReviews	Gene locus BTH_I1824 in contig CP000086_GR.
KEGG	bte:BTH_I1824.
PATRIC	19306654. VBIBurTha36512_4624.
TIGR	BTH_I1824.
Phylogenomic databases
eggNOG	COG1228.
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1824-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BURTA

Accession

Primary (citable) accession number: Q2SXJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program