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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi418Magnesium 1UniRule annotation1
Metal bindingi425Magnesium 1UniRule annotation1
Metal bindingi425Magnesium 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligaseUniRule annotation (EC:6.1.1.6UniRule annotation)
Alternative name(s):
Lysyl-tRNA synthetaseUniRule annotation
Short name:
LysRSUniRule annotation
Gene namesi
Name:lysSUniRule annotation
Ordered Locus Names:BTH_I1883
OrganismiBurkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000001930 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000128591 – 508Lysine--tRNA ligaseAdd BLAST508

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 31Combined sources16
Helixi46 – 52Combined sources7
Turni53 – 55Combined sources3
Helixi58 – 64Combined sources7
Beta strandi67 – 80Combined sources14
Beta strandi83 – 89Combined sources7
Beta strandi94 – 99Combined sources6
Helixi101 – 104Combined sources4
Helixi106 – 113Combined sources8
Beta strandi120 – 129Combined sources10
Beta strandi135 – 146Combined sources12
Helixi164 – 167Combined sources4
Helixi169 – 175Combined sources7
Helixi177 – 199Combined sources23
Beta strandi209 – 213Combined sources5
Beta strandi216 – 219Combined sources4
Beta strandi223 – 226Combined sources4
Turni227 – 230Combined sources4
Beta strandi231 – 235Combined sources5
Helixi240 – 248Combined sources9
Beta strandi252 – 261Combined sources10
Beta strandi272 – 282Combined sources11
Helixi286 – 305Combined sources20
Beta strandi308 – 312Combined sources5
Beta strandi315 – 318Combined sources4
Beta strandi324 – 327Combined sources4
Helixi328 – 335Combined sources8
Helixi341 – 344Combined sources4
Helixi347 – 356Combined sources10
Helixi364 – 366Combined sources3
Helixi371 – 382Combined sources12
Helixi384 – 386Combined sources3
Beta strandi391 – 397Combined sources7
Helixi398 – 400Combined sources3
Beta strandi408 – 410Combined sources3
Beta strandi413 – 421Combined sources9
Beta strandi424 – 432Combined sources9
Helixi436 – 451Combined sources16
Helixi462 – 469Combined sources8
Beta strandi475 – 481Combined sources7
Helixi482 – 490Combined sources9
Helixi495 – 498Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EX5X-ray2.40A/B1-508[»]
ProteinModelPortaliQ2SXD6.
SMRiQ2SXD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiPOG091H0098.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2SXD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPIQPQAA VAADENQIVA ERRDKLRALR DQGIAYPNDF QPTHHAADLQ
60 70 80 90 100
TAYADADKEA LEAKSLEVAI AGRMMLKRVM GKASFATVQD GSGQIQFFVT
110 120 130 140 150
PADVGAETYD AFKKWDLGDI VAARGVLFRT NKGELSVKCT QLRLLAKALR
160 170 180 190 200
PLPDKFHGLA DQETRYRQRY VDLIVTPETR TTFRARTKAI ASIRKFMGDA
210 220 230 240 250
DFMEVETPML HPIPGGAAAK PFVTHHNALD MEMFLRIAPE LYLKRLIVGG
260 270 280 290 300
FERVFEINRN FRNEGVSPRH NPEFTMMEFY AAYTDYRWLM DFTERLIRQA
310 320 330 340 350
AVDALGTATI QYQGRELDLA QPFHRLTITQ AIQKYAPSYT DGQLSDDAFL
360 370 380 390 400
RSELKRLGVD VTQPAFLNAG IGALQLALFE ETAEAQLWEP TFIIDYPIEV
410 420 430 440 450
SPLARESDTV AGITERFELF ITGREIANGF SELNDPEDQA ARFKKQVEQK
460 470 480 490 500
DAGDEEAMFF DADYIRALEY GMPPTGGCGI GIDRLVMLLT DSPTIRDVLL

FPHLRRED
Length:508
Mass (Da):57,233
Last modified:January 24, 2006 - v1
Checksum:i6AC3B72F4B0FA765
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37378.1.
RefSeqiWP_009890173.1. NZ_CM000438.1.

Genome annotation databases

EnsemblBacteriaiABC37378; ABC37378; BTH_I1883.
KEGGibte:BTH_I1883.
PATRICi19306790. VBIBurTha36512_4690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37378.1.
RefSeqiWP_009890173.1. NZ_CM000438.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EX5X-ray2.40A/B1-508[»]
ProteinModelPortaliQ2SXD6.
SMRiQ2SXD6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC37378; ABC37378; BTH_I1883.
KEGGibte:BTH_I1883.
PATRICi19306790. VBIBurTha36512_4690.

Phylogenomic databases

HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiPOG091H0098.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYK_BURTA
AccessioniPrimary (citable) accession number: Q2SXD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.