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Q2SXD6 (SYK_BURTA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase
EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:lysS
Ordered Locus Names:BTH_I1883
OrganismBurkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]
Taxonomic identifier271848 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group ›

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252

Cofactor

Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_00252

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00252

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Lysine--tRNA ligase HAMAP-Rule MF_00252
PRO_1000012859

Sites

Metal binding4181Magnesium 1 By similarity
Metal binding4251Magnesium 1 By similarity
Metal binding4251Magnesium 2 By similarity

Secondary structure

1 ............................................................................... 508
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q2SXD6 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 6AC3B72F4B0FA765

FASTA50857,233
        10         20         30         40         50         60 
MTEPIQPQAA VAADENQIVA ERRDKLRALR DQGIAYPNDF QPTHHAADLQ TAYADADKEA 

        70         80         90        100        110        120 
LEAKSLEVAI AGRMMLKRVM GKASFATVQD GSGQIQFFVT PADVGAETYD AFKKWDLGDI 

       130        140        150        160        170        180 
VAARGVLFRT NKGELSVKCT QLRLLAKALR PLPDKFHGLA DQETRYRQRY VDLIVTPETR 

       190        200        210        220        230        240 
TTFRARTKAI ASIRKFMGDA DFMEVETPML HPIPGGAAAK PFVTHHNALD MEMFLRIAPE 

       250        260        270        280        290        300 
LYLKRLIVGG FERVFEINRN FRNEGVSPRH NPEFTMMEFY AAYTDYRWLM DFTERLIRQA 

       310        320        330        340        350        360 
AVDALGTATI QYQGRELDLA QPFHRLTITQ AIQKYAPSYT DGQLSDDAFL RSELKRLGVD 

       370        380        390        400        410        420 
VTQPAFLNAG IGALQLALFE ETAEAQLWEP TFIIDYPIEV SPLARESDTV AGITERFELF 

       430        440        450        460        470        480 
ITGREIANGF SELNDPEDQA ARFKKQVEQK DAGDEEAMFF DADYIRALEY GMPPTGGCGI 

       490        500 
GIDRLVMLLT DSPTIRDVLL FPHLRRED

« Hide

References

[1]"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies."
Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C., DeShazer D.
BMC Genomics 6:174-174(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: E264 / ATCC 700388 / DSM 13276 / CIP 106301.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000086 Genomic DNA. Translation: ABC37378.1.
RefSeqYP_442413.1. NC_007651.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EX5X-ray2.40A/B1-508[»]
ProteinModelPortalQ2SXD6.
SMRQ2SXD6. Positions 13-505.
ModBaseSearch...

Protein-protein interaction databases

STRING271848.BTH_I1883.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC37378; ABC37378; BTH_I1883.
GeneID3849089.
KEGGbte:BTH_I1883.
PATRIC19306790. VBIBurTha36512_4690.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1190.
HOGENOMHOG000236578.
KOK04567.
OMANTAANIK.
ProtClustDBPRK00484.

Enzyme and pathway databases

BioCycBTHA271848:GJMY-1883-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00252. Lys_tRNA_synth_class2.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF4. PTHR22594:SF4. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK_BURTA
AccessionPrimary (citable) accession number: Q2SXD6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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