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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi418 – 4181Magnesium 1UniRule annotation
Metal bindingi425 – 4251Magnesium 1UniRule annotation
Metal bindingi425 – 4251Magnesium 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHA271848:GJMY-1883-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligaseUniRule annotation (EC:6.1.1.6UniRule annotation)
Alternative name(s):
Lysyl-tRNA synthetaseUniRule annotation
Short name:
LysRSUniRule annotation
Gene namesi
Name:lysSUniRule annotation
Ordered Locus Names:BTH_I1883
OrganismiBurkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Taxonomic identifieri271848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000001930 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508Lysine--tRNA ligasePRO_1000012859Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1
508
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi16 – 3116Combined sources
Helixi46 – 527Combined sources
Turni53 – 553Combined sources
Helixi58 – 647Combined sources
Beta strandi67 – 8014Combined sources
Beta strandi83 – 897Combined sources
Beta strandi94 – 996Combined sources
Helixi101 – 1044Combined sources
Helixi106 – 1138Combined sources
Beta strandi120 – 12910Combined sources
Beta strandi135 – 14612Combined sources
Helixi164 – 1674Combined sources
Helixi169 – 1757Combined sources
Helixi177 – 19923Combined sources
Beta strandi209 – 2135Combined sources
Beta strandi216 – 2194Combined sources
Beta strandi223 – 2264Combined sources
Turni227 – 2304Combined sources
Beta strandi231 – 2355Combined sources
Helixi240 – 2489Combined sources
Beta strandi252 – 26110Combined sources
Beta strandi272 – 28211Combined sources
Helixi286 – 30520Combined sources
Beta strandi308 – 3125Combined sources
Beta strandi315 – 3184Combined sources
Beta strandi324 – 3274Combined sources
Helixi328 – 3358Combined sources
Helixi341 – 3444Combined sources
Helixi347 – 35610Combined sources
Helixi364 – 3663Combined sources
Helixi371 – 38212Combined sources
Helixi384 – 3863Combined sources
Beta strandi391 – 3977Combined sources
Helixi398 – 4003Combined sources
Beta strandi408 – 4103Combined sources
Beta strandi413 – 4219Combined sources
Beta strandi424 – 4329Combined sources
Helixi436 – 45116Combined sources
Helixi462 – 4698Combined sources
Beta strandi475 – 4817Combined sources
Helixi482 – 4909Combined sources
Helixi495 – 4984Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EX5X-ray2.40A/B1-508[»]
ProteinModelPortaliQ2SXD6.
SMRiQ2SXD6. Positions 13-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiPOG091H0098.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2SXD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPIQPQAA VAADENQIVA ERRDKLRALR DQGIAYPNDF QPTHHAADLQ
60 70 80 90 100
TAYADADKEA LEAKSLEVAI AGRMMLKRVM GKASFATVQD GSGQIQFFVT
110 120 130 140 150
PADVGAETYD AFKKWDLGDI VAARGVLFRT NKGELSVKCT QLRLLAKALR
160 170 180 190 200
PLPDKFHGLA DQETRYRQRY VDLIVTPETR TTFRARTKAI ASIRKFMGDA
210 220 230 240 250
DFMEVETPML HPIPGGAAAK PFVTHHNALD MEMFLRIAPE LYLKRLIVGG
260 270 280 290 300
FERVFEINRN FRNEGVSPRH NPEFTMMEFY AAYTDYRWLM DFTERLIRQA
310 320 330 340 350
AVDALGTATI QYQGRELDLA QPFHRLTITQ AIQKYAPSYT DGQLSDDAFL
360 370 380 390 400
RSELKRLGVD VTQPAFLNAG IGALQLALFE ETAEAQLWEP TFIIDYPIEV
410 420 430 440 450
SPLARESDTV AGITERFELF ITGREIANGF SELNDPEDQA ARFKKQVEQK
460 470 480 490 500
DAGDEEAMFF DADYIRALEY GMPPTGGCGI GIDRLVMLLT DSPTIRDVLL

FPHLRRED
Length:508
Mass (Da):57,233
Last modified:January 24, 2006 - v1
Checksum:i6AC3B72F4B0FA765
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37378.1.
RefSeqiWP_009890173.1. NZ_CM000438.1.

Genome annotation databases

EnsemblBacteriaiABC37378; ABC37378; BTH_I1883.
KEGGibte:BTH_I1883.
PATRICi19306790. VBIBurTha36512_4690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000086 Genomic DNA. Translation: ABC37378.1.
RefSeqiWP_009890173.1. NZ_CM000438.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EX5X-ray2.40A/B1-508[»]
ProteinModelPortaliQ2SXD6.
SMRiQ2SXD6. Positions 13-505.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC37378; ABC37378; BTH_I1883.
KEGGibte:BTH_I1883.
PATRICi19306790. VBIBurTha36512_4690.

Phylogenomic databases

HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiPOG091H0098.

Enzyme and pathway databases

BioCyciBTHA271848:GJMY-1883-MONOMER.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYK_BURTA
AccessioniPrimary (citable) accession number: Q2SXD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 24, 2006
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.