Reviewed, UniProtKB/Swiss-Prot Q2SXC7 (PYRG_BURTA)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	BTH_I1892

Organism

Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) [Complete proteome] [HAMAP]

Taxonomic identifier

271848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › pseudomallei group

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	CTP synthase HAMAP MF_01227		PRO_0000266086
Regions
	Domain	295 – 547	253	Glutamine amidotransferase type-1
	Region	1 – 257	257	Aminator domain HAMAP MF_01227
Sites
	Active site	383	1	Nucleophile By similarity
	Active site	520	1	By similarity
	Active site	522	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SXC7-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 5D1CB23AAC3FF8F6	FASTA	553	61,095
	10 20 30 40 50 60 MTKYVFVTGG VVSSLGKGIA AASLAAILES RGLKVTLLKL DPYINVDPGT MSPFQHGEVF 70 80 90 100 110 120 VTEDGAETDL DLGHYERFIS TKMRKANNFT TGQIYESVIR KERRGDYLGK TVQVIPHITN 130 140 150 160 170 180 EIQAFIERGA ASATCGEPDV AIVEIGGTVG DIESLPFLEA ARQMSLRLGR NSACFVHLTL 190 200 210 220 230 240 VPFIATAGEL KTKPTQHSVQ KLREIGISPH VLLCRADRPI PDDESKKISL FSNVPEDAVI 250 260 270 280 290 300 SVWDVDSIYK IPQMLHDQGL DRLICEELRL DPQPADLRMW SELVEKLQNP KHEVTIGMVG 310 320 330 340 350 360 KYVDLTESYK SLIEALRHAS IHTSTKVNIE YIDSEELEAN GTASLAHLDA VLVPGGFGRR 370 380 390 400 410 420 GTEGKIAAVR YAREAKVPYL GICLGMQLAV IEFARDVVGL KQANSTEFDP NTPERVVALI 430 440 450 460 470 480 TEWYDREGKV EKRTEDSDLG GTMRLGSQRC PIKPGTLAEA IYGKDVNERH RHRYEVNNRF 490 500 510 520 530 540 VPQLEAGGLV ISARTPSEDL PEMMELPRAM HPWFVGVQFH PEFTSTPRDG HPLFKSFVQA 550 AIACQQARAG AKA

References

[1]

Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A., Lathigra R., White O., Ketchum K.A., Palmer N., Dodson R., Hickey E.K., Gwinn M.L., Dougherty B., Fleischmann R.D., Richardson D.L., Peterson J.D., Kerlavage A.R., Quackenbush J. , Salzberg S.L., Hanson M., van-Vugt R., Adams M.D., Gocayne J.D., Weidman J., Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C., Garland S., Fujii C., Cotton M.D., Horst K., Tomb J.-F., Roberts K., Hatch B., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000086 Genomic DNA. Translation: ABC37894.1.
RefSeq	YP_442422.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3848784.
GenomeReviews	Gene locus BTH_I1892 in contig CP000086_GR.
KEGG	bte:BTH_I1892.
TIGR	BTH_I1892.
Phylogenomic databases
HOGENOM	Q2SXC7.
OMA	Q2SXC7. EFNNAYR.
Enzyme and pathway databases
BioCyc	BTHA271848:BTH_I1892-MON.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_BURTA

Accession

Primary (citable) accession number: Q2SXC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)