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Q2SSZ1 (SYE_MYCCT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MCAP_0130
OrganismMycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]
Taxonomic identifier340047 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000237371

Regions

Motif9 – 1911"HIGH" region HAMAP-Rule MF_00022
Motif253 – 2575"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2561ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2SSZ1 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 443CE73C7AF147F6

FASTA48356,433
        10         20         30         40         50         60 
MSFRLRYAPS PTGFLHIGNT RTALMNYLFA KHYNGSFIVR IEDTDLARNV EGAIESQFEN 

        70         80         90        100        110        120 
LNWLGIYPDE SIFNPNDQKY GKYMQSQKFD RYKQLAEQLV DQNKAYRCFC TSEELEKDYE 

       130        140        150        160        170        180 
DQTSKGIIAT KYSQKCLFLT QDQIQKNLEN KKEYSIRFKV PKNKTWTIND IVRGDVSFDS 

       190        200        210        220        230        240 
KDLGDFVILK SNGVATYNFA VVVDDYDMQI THVLRGEEHI SNTPRQMMIY DAFNWNYPMF 

       250        260        270        280        290        300 
GHLTLIVDNT GKKLSKRSGN ALFFIEQYKK QGYLSQAIFN YIALLGWSPP GEQEILSQNE 

       310        320        330        340        350        360 
LIKIFDEKRF SKSPSTFDMV KMKWINSVYM KKLDDDKYLE FVKSFINTNK FDITSKSETW 

       370        380        390        400        410        420 
LNHLLLLYKK ELEYAEQIND HLDLFFNKNT LDNNTIDVLN NLTNYKNVVE IFKNQINVLK 

       430        440        450        460        470        480 
DWTIENIKQI IKDTSTLANV KGKDLFMPIR IFATKSEHGP SLADVIYLLG KTTVLNNINS 


LER 

« Hide

References

[1]Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: California kid / ATCC 27343 / NCTC 10154.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000123 Genomic DNA. Translation: ABC01842.1.
RefSeqYP_424122.1. NC_007633.1.

3D structure databases

ProteinModelPortalQ2SSZ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING340047.MCAP_0130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC01842; ABC01842; MCAP_0130.
GeneID3828322.
KEGGmcp:MCAP_0130.
PATRIC20004717. VBIMycCap130493_0129.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAAFRCFCT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycMCAP340047:GC0H-129-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_MYCCT
AccessionPrimary (citable) accession number: Q2SSZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: January 24, 2006
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries