Q2SSQ6 (K6PF_MYCCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	MCAP_0220

Organism

Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) [Complete proteome] [HAMAP]

Taxonomic identifier

340047 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma mycoides group › Mycoplasma capricolum › Mycoplasma capricolum subsp. capricolum

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_1000059778
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2SSQ6 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 558F7448CBFCC949	FASTA	326	35,179
	10 20 30 40 50 60 MIKKIGILTS GGDAPGMNNA IAGVVKTASS KGIEVYGIND GYKGLVNGWF EKLNVEKTLD 70 80 90 100 110 120 ILSRGGTYLG SARLPEFKEL EVRQKAVANL KKAGIEALVV IGGDGSYMGA QRLTEMGINC 130 140 150 160 170 180 IGLPGTIDND IASTDYTIGF HTALNTIVEA VDRIRDTMQS HNRADVVEIM GNGCGDLVTY 190 200 210 220 230 240 TAVATGAEIF SPAEDLLTIE QMGQKAKQFR LLGKKSLIIL VSEKLYGISA EEIAEKIQKI 250 260 270 280 290 300 SGYETKATVL GHIQRGGRPT AMDRYIAFTA GMFAVEKLAE GKGGLFIGLE NNKLVARDID 310 320 STLNMKKEDK KPFIKNLREI NGHFSK

References

[1]

Glass J.I., Lartigue C., Pfannkoch C., Baden-Tillson H., Smith H.O., Venter J.C., Roske K., Wise K.S., Calcutt M.J., Nelson W.C., Nierman W.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: California kid / ATCC 27343 / NCTC 10154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000123 Genomic DNA. Translation: ABC01496.1.
RefSeq	YP_424207.1. NC_007633.1.
3D structure databases
HSSP	HSSP built from PDB template 2PFK based on UniProtKB P0A796.
ProteinModelPortal	Q2SSQ6.
SMR	Q2SSQ6. Positions 1-314.
ModBase	Search...
Protein-protein interaction databases
STRING	340047.MCAP_0220.
Proteomic databases
PRIDE	Q2SSQ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC01496; ABC01496; MCAP_0220.
GeneID	3829069.
KEGG	mcp:MCAP_0220.
PATRIC	20004905. VBIMycCap130493_0223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	QKENINA.
ProtClustDB	PRK03202.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_MYCCT

Accession

Primary (citable) accession number: Q2SSQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program